Experiment: PYE with CHIR-090 10 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_01679 and CCNA_01680 overlap by 4 nucleotides CCNA_01680 and CCNA_01681 overlap by 4 nucleotides CCNA_01681 and CCNA_01682 overlap by 4 nucleotides
CCNA_01679: CCNA_01679 - succinylglutamic semialdehyde dehydrogenase, at 1,806,060 to 1,807,517
_01679
CCNA_01680: CCNA_01680 - succinylarginine dihydrolase, at 1,807,514 to 1,808,854
_01680
CCNA_01681: CCNA_01681 - RimI-like acetyltransferase, at 1,808,851 to 1,809,357
_01681
CCNA_01682: CCNA_01682 - LytT-family transcriptional regulator, at 1,809,354 to 1,810,292
_01682
Position (kb)
1807
1808
1809 Strain fitness (log2 ratio)
-2
-1
0
1 at 1806.571 kb on - strand, within CCNA_01679 at 1806.571 kb on - strand, within CCNA_01679 at 1806.676 kb on + strand, within CCNA_01679 at 1806.774 kb on - strand, within CCNA_01679 at 1806.870 kb on + strand, within CCNA_01679 at 1806.893 kb on - strand, within CCNA_01679 at 1806.985 kb on - strand, within CCNA_01679 at 1807.013 kb on + strand, within CCNA_01679 at 1807.087 kb on - strand, within CCNA_01679 at 1807.113 kb on + strand, within CCNA_01679 at 1807.113 kb on + strand, within CCNA_01679 at 1807.113 kb on + strand, within CCNA_01679 at 1807.113 kb on + strand, within CCNA_01679 at 1807.113 kb on + strand, within CCNA_01679 at 1807.121 kb on - strand, within CCNA_01679 at 1807.121 kb on - strand, within CCNA_01679 at 1807.144 kb on - strand, within CCNA_01679 at 1807.166 kb on - strand, within CCNA_01679 at 1807.184 kb on + strand, within CCNA_01679 at 1807.369 kb on + strand, within CCNA_01679 at 1807.369 kb on + strand, within CCNA_01679 at 1807.369 kb on + strand, within CCNA_01679 at 1807.377 kb on - strand at 1807.444 kb on - strand at 1807.444 kb on - strand at 1807.547 kb on - strand at 1807.557 kb on - strand at 1807.557 kb on - strand at 1807.564 kb on + strand at 1807.572 kb on - strand at 1807.572 kb on - strand at 1807.642 kb on + strand at 1807.685 kb on - strand, within CCNA_01680 at 1807.721 kb on + strand, within CCNA_01680 at 1807.777 kb on - strand, within CCNA_01680 at 1807.794 kb on + strand, within CCNA_01680 at 1807.824 kb on - strand, within CCNA_01680 at 1807.909 kb on + strand, within CCNA_01680 at 1807.909 kb on + strand, within CCNA_01680 at 1808.059 kb on + strand, within CCNA_01680 at 1808.059 kb on + strand, within CCNA_01680 at 1808.062 kb on - strand, within CCNA_01680 at 1808.067 kb on - strand, within CCNA_01680 at 1808.079 kb on + strand, within CCNA_01680 at 1808.084 kb on + strand, within CCNA_01680 at 1808.087 kb on - strand, within CCNA_01680 at 1808.107 kb on + strand, within CCNA_01680 at 1808.218 kb on + strand, within CCNA_01680 at 1808.218 kb on + strand, within CCNA_01680 at 1808.226 kb on - strand, within CCNA_01680 at 1808.226 kb on - strand, within CCNA_01680 at 1808.260 kb on + strand, within CCNA_01680 at 1808.273 kb on - strand, within CCNA_01680 at 1808.359 kb on + strand, within CCNA_01680 at 1808.379 kb on + strand, within CCNA_01680 at 1808.391 kb on - strand, within CCNA_01680 at 1808.421 kb on - strand, within CCNA_01680 at 1808.509 kb on + strand, within CCNA_01680 at 1808.522 kb on + strand, within CCNA_01680 at 1808.620 kb on + strand, within CCNA_01680 at 1808.640 kb on + strand, within CCNA_01680 at 1808.640 kb on + strand, within CCNA_01680 at 1808.747 kb on + strand at 1808.766 kb on - strand at 1808.771 kb on + strand at 1808.778 kb on + strand at 1808.786 kb on - strand at 1808.808 kb on - strand at 1808.827 kb on + strand at 1808.835 kb on - strand at 1808.835 kb on - strand at 1808.836 kb on + strand at 1808.910 kb on - strand, within CCNA_01681 at 1808.910 kb on - strand, within CCNA_01681 at 1808.913 kb on + strand, within CCNA_01681 at 1808.913 kb on + strand, within CCNA_01681 at 1808.998 kb on - strand, within CCNA_01681 at 1809.056 kb on - strand, within CCNA_01681 at 1809.070 kb on + strand, within CCNA_01681 at 1809.100 kb on - strand, within CCNA_01681 at 1809.113 kb on - strand, within CCNA_01681 at 1809.118 kb on - strand, within CCNA_01681 at 1809.137 kb on + strand, within CCNA_01681 at 1809.250 kb on + strand, within CCNA_01681 at 1809.433 kb on - strand at 1809.454 kb on + strand, within CCNA_01682 at 1809.513 kb on + strand, within CCNA_01682 at 1809.513 kb on + strand, within CCNA_01682 at 1809.521 kb on - strand, within CCNA_01682 at 1809.598 kb on - strand, within CCNA_01682 at 1809.600 kb on + strand, within CCNA_01682 at 1809.650 kb on + strand, within CCNA_01682
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with CHIR-090 10 ug/mL remove 1,806,571 - CCNA_01679 0.35 +0.7 1,806,571 - CCNA_01679 0.35 -1.7 1,806,676 + CCNA_01679 0.42 +0.4 1,806,774 - CCNA_01679 0.49 -0.4 1,806,870 + CCNA_01679 0.56 -0.2 1,806,893 - CCNA_01679 0.57 -0.2 1,806,985 - CCNA_01679 0.63 +1.0 1,807,013 + CCNA_01679 0.65 +0.3 1,807,087 - CCNA_01679 0.70 -0.6 1,807,113 + CCNA_01679 0.72 +0.3 1,807,113 + CCNA_01679 0.72 -0.2 1,807,113 + CCNA_01679 0.72 +0.4 1,807,113 + CCNA_01679 0.72 +0.3 1,807,113 + CCNA_01679 0.72 +0.6 1,807,121 - CCNA_01679 0.73 +0.2 1,807,121 - CCNA_01679 0.73 +0.4 1,807,144 - CCNA_01679 0.74 -0.5 1,807,166 - CCNA_01679 0.76 -0.5 1,807,184 + CCNA_01679 0.77 -0.6 1,807,369 + CCNA_01679 0.90 +0.3 1,807,369 + CCNA_01679 0.90 +0.9 1,807,369 + CCNA_01679 0.90 +0.1 1,807,377 - +1.6 1,807,444 - -1.1 1,807,444 - +1.6 1,807,547 - +0.3 1,807,557 - -0.1 1,807,557 - -1.8 1,807,564 + +0.4 1,807,572 - -1.1 1,807,572 - -0.1 1,807,642 + +0.1 1,807,685 - CCNA_01680 0.13 -0.2 1,807,721 + CCNA_01680 0.15 +0.0 1,807,777 - CCNA_01680 0.20 +1.6 1,807,794 + CCNA_01680 0.21 +0.3 1,807,824 - CCNA_01680 0.23 +0.6 1,807,909 + CCNA_01680 0.29 +0.2 1,807,909 + CCNA_01680 0.29 -0.6 1,808,059 + CCNA_01680 0.41 +1.3 1,808,059 + CCNA_01680 0.41 +0.9 1,808,062 - CCNA_01680 0.41 +1.2 1,808,067 - CCNA_01680 0.41 -0.3 1,808,079 + CCNA_01680 0.42 +0.3 1,808,084 + CCNA_01680 0.43 -0.2 1,808,087 - CCNA_01680 0.43 -0.1 1,808,107 + CCNA_01680 0.44 +0.8 1,808,218 + CCNA_01680 0.52 +0.4 1,808,218 + CCNA_01680 0.52 +0.4 1,808,226 - CCNA_01680 0.53 +0.5 1,808,226 - CCNA_01680 0.53 +1.4 1,808,260 + CCNA_01680 0.56 +0.8 1,808,273 - CCNA_01680 0.57 -2.1 1,808,359 + CCNA_01680 0.63 -0.5 1,808,379 + CCNA_01680 0.65 +0.1 1,808,391 - CCNA_01680 0.65 +1.0 1,808,421 - CCNA_01680 0.68 +1.0 1,808,509 + CCNA_01680 0.74 +1.5 1,808,522 + CCNA_01680 0.75 -0.1 1,808,620 + CCNA_01680 0.82 +1.4 1,808,640 + CCNA_01680 0.84 +0.4 1,808,640 + CCNA_01680 0.84 -0.6 1,808,747 + +0.7 1,808,766 - -0.8 1,808,771 + +0.3 1,808,778 + +0.2 1,808,786 - +1.4 1,808,808 - +0.7 1,808,827 + -0.8 1,808,835 - -0.6 1,808,835 - -0.5 1,808,836 + +0.3 1,808,910 - CCNA_01681 0.12 -1.3 1,808,910 - CCNA_01681 0.12 +0.2 1,808,913 + CCNA_01681 0.12 +0.3 1,808,913 + CCNA_01681 0.12 +0.4 1,808,998 - CCNA_01681 0.29 +0.0 1,809,056 - CCNA_01681 0.40 +0.6 1,809,070 + CCNA_01681 0.43 -0.5 1,809,100 - CCNA_01681 0.49 -0.7 1,809,113 - CCNA_01681 0.52 -2.2 1,809,118 - CCNA_01681 0.53 +0.8 1,809,137 + CCNA_01681 0.56 +0.0 1,809,250 + CCNA_01681 0.79 -0.5 1,809,433 - +0.0 1,809,454 + CCNA_01682 0.11 +0.0 1,809,513 + CCNA_01682 0.17 -0.7 1,809,513 + CCNA_01682 0.17 +0.1 1,809,521 - CCNA_01682 0.18 +0.2 1,809,598 - CCNA_01682 0.26 +1.2 1,809,600 + CCNA_01682 0.26 +0.7 1,809,650 + CCNA_01682 0.32 -1.9
Or see this region's nucleotide sequence