Experiment: PYE with CHIR-090 10 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_01653 and CCNA_01654 overlap by 4 nucleotides CCNA_01654 and CCNA_01655 are separated by 91 nucleotides CCNA_01655 and CCNA_01656 are separated by 166 nucleotides
CCNA_01653: CCNA_01653 - cyclophilin-type peptidylprolyl cis-trans isomerase, at 1,774,739 to 1,775,572
_01653
CCNA_01654: CCNA_01654 - cyclophilin-type peptidylprolyl cis-trans isomerase, at 1,775,569 to 1,776,417
_01654
CCNA_01655: CCNA_01655 - cyclophilin-type peptidylprolyl cis-trans isomerase, at 1,776,509 to 1,776,976
_01655
CCNA_01656: CCNA_01656 - endonuclease/exonuclease/phosphatase family protein, at 1,777,143 to 1,778,120
_01656
Position (kb)
1776
1777 Strain fitness (log2 ratio)
-2
-1
0
1 at 1775.761 kb on - strand, within CCNA_01654 at 1775.805 kb on - strand, within CCNA_01654 at 1775.842 kb on + strand, within CCNA_01654 at 1775.849 kb on + strand, within CCNA_01654 at 1775.936 kb on + strand, within CCNA_01654 at 1775.936 kb on + strand, within CCNA_01654 at 1775.963 kb on + strand, within CCNA_01654 at 1775.963 kb on + strand, within CCNA_01654 at 1775.971 kb on - strand, within CCNA_01654 at 1775.978 kb on + strand, within CCNA_01654 at 1776.001 kb on + strand, within CCNA_01654 at 1776.043 kb on + strand, within CCNA_01654 at 1776.060 kb on + strand, within CCNA_01654 at 1776.067 kb on - strand, within CCNA_01654 at 1776.097 kb on + strand, within CCNA_01654 at 1776.146 kb on - strand, within CCNA_01654 at 1776.159 kb on + strand, within CCNA_01654 at 1776.204 kb on - strand, within CCNA_01654 at 1776.287 kb on - strand, within CCNA_01654 at 1776.350 kb on - strand at 1776.454 kb on - strand at 1776.476 kb on - strand at 1776.555 kb on + strand at 1776.745 kb on - strand, within CCNA_01655 at 1776.782 kb on - strand, within CCNA_01655 at 1776.782 kb on - strand, within CCNA_01655 at 1776.803 kb on - strand, within CCNA_01655 at 1776.812 kb on + strand, within CCNA_01655 at 1776.827 kb on + strand, within CCNA_01655 at 1776.856 kb on + strand, within CCNA_01655 at 1776.864 kb on - strand, within CCNA_01655 at 1776.866 kb on + strand, within CCNA_01655 at 1776.908 kb on + strand, within CCNA_01655 at 1776.916 kb on - strand, within CCNA_01655 at 1776.918 kb on - strand, within CCNA_01655 at 1777.008 kb on - strand at 1777.008 kb on - strand at 1777.069 kb on + strand at 1777.110 kb on - strand at 1777.116 kb on + strand at 1777.116 kb on + strand at 1777.208 kb on + strand at 1777.210 kb on - strand at 1777.210 kb on - strand at 1777.281 kb on - strand, within CCNA_01656 at 1777.294 kb on + strand, within CCNA_01656 at 1777.486 kb on + strand, within CCNA_01656 at 1777.494 kb on - strand, within CCNA_01656 at 1777.560 kb on - strand, within CCNA_01656 at 1777.562 kb on + strand, within CCNA_01656 at 1777.562 kb on + strand, within CCNA_01656 at 1777.570 kb on - strand, within CCNA_01656 at 1777.573 kb on + strand, within CCNA_01656 at 1777.607 kb on + strand, within CCNA_01656 at 1777.608 kb on + strand, within CCNA_01656 at 1777.623 kb on - strand, within CCNA_01656 at 1777.623 kb on - strand, within CCNA_01656 at 1777.658 kb on - strand, within CCNA_01656 at 1777.782 kb on - strand, within CCNA_01656 at 1777.788 kb on - strand, within CCNA_01656 at 1777.795 kb on + strand, within CCNA_01656 at 1777.796 kb on - strand, within CCNA_01656 at 1777.851 kb on - strand, within CCNA_01656 at 1777.865 kb on + strand, within CCNA_01656 at 1777.865 kb on + strand, within CCNA_01656 at 1777.870 kb on + strand, within CCNA_01656 at 1777.878 kb on - strand, within CCNA_01656 at 1777.897 kb on + strand, within CCNA_01656 at 1777.900 kb on - strand, within CCNA_01656 at 1777.906 kb on + strand, within CCNA_01656 at 1777.950 kb on - strand, within CCNA_01656
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with CHIR-090 10 ug/mL remove 1,775,761 - CCNA_01654 0.23 -1.5 1,775,805 - CCNA_01654 0.28 -0.2 1,775,842 + CCNA_01654 0.32 +0.4 1,775,849 + CCNA_01654 0.33 +0.9 1,775,936 + CCNA_01654 0.43 +1.0 1,775,936 + CCNA_01654 0.43 +0.6 1,775,963 + CCNA_01654 0.46 +0.4 1,775,963 + CCNA_01654 0.46 -0.5 1,775,971 - CCNA_01654 0.47 +0.9 1,775,978 + CCNA_01654 0.48 -0.1 1,776,001 + CCNA_01654 0.51 +0.2 1,776,043 + CCNA_01654 0.56 +0.2 1,776,060 + CCNA_01654 0.58 +0.3 1,776,067 - CCNA_01654 0.59 -0.4 1,776,097 + CCNA_01654 0.62 +0.6 1,776,146 - CCNA_01654 0.68 +0.7 1,776,159 + CCNA_01654 0.69 +0.2 1,776,204 - CCNA_01654 0.75 +0.6 1,776,287 - CCNA_01654 0.85 -0.2 1,776,350 - -2.3 1,776,454 - +0.8 1,776,476 - -0.6 1,776,555 + -0.1 1,776,745 - CCNA_01655 0.50 +1.4 1,776,782 - CCNA_01655 0.58 +0.1 1,776,782 - CCNA_01655 0.58 -0.2 1,776,803 - CCNA_01655 0.63 +0.2 1,776,812 + CCNA_01655 0.65 +0.5 1,776,827 + CCNA_01655 0.68 +0.6 1,776,856 + CCNA_01655 0.74 +0.6 1,776,864 - CCNA_01655 0.76 +0.5 1,776,866 + CCNA_01655 0.76 +0.5 1,776,908 + CCNA_01655 0.85 +1.6 1,776,916 - CCNA_01655 0.87 -1.7 1,776,918 - CCNA_01655 0.87 +0.4 1,777,008 - -0.4 1,777,008 - +0.8 1,777,069 + +0.5 1,777,110 - +0.2 1,777,116 + +0.5 1,777,116 + -0.2 1,777,208 + +0.2 1,777,210 - +1.6 1,777,210 - -0.2 1,777,281 - CCNA_01656 0.14 +0.0 1,777,294 + CCNA_01656 0.15 +1.1 1,777,486 + CCNA_01656 0.35 +1.4 1,777,494 - CCNA_01656 0.36 +1.5 1,777,560 - CCNA_01656 0.43 +0.7 1,777,562 + CCNA_01656 0.43 +0.6 1,777,562 + CCNA_01656 0.43 +0.5 1,777,570 - CCNA_01656 0.44 -0.2 1,777,573 + CCNA_01656 0.44 -0.4 1,777,607 + CCNA_01656 0.47 +0.4 1,777,608 + CCNA_01656 0.48 -1.3 1,777,623 - CCNA_01656 0.49 -0.2 1,777,623 - CCNA_01656 0.49 -0.9 1,777,658 - CCNA_01656 0.53 -0.6 1,777,782 - CCNA_01656 0.65 -0.5 1,777,788 - CCNA_01656 0.66 +0.3 1,777,795 + CCNA_01656 0.67 +0.3 1,777,796 - CCNA_01656 0.67 +0.6 1,777,851 - CCNA_01656 0.72 -0.7 1,777,865 + CCNA_01656 0.74 -0.1 1,777,865 + CCNA_01656 0.74 -0.3 1,777,870 + CCNA_01656 0.74 +0.5 1,777,878 - CCNA_01656 0.75 +0.3 1,777,897 + CCNA_01656 0.77 +1.4 1,777,900 - CCNA_01656 0.77 -0.4 1,777,906 + CCNA_01656 0.78 +0.3 1,777,950 - CCNA_01656 0.83 -1.6
Or see this region's nucleotide sequence