Experiment: PYE with CHIR-090 10 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_01651 and CCNA_01652 are separated by 26 nucleotides CCNA_01652 and CCNA_01653 are separated by 157 nucleotides CCNA_01653 and CCNA_01654 overlap by 4 nucleotides CCNA_01654 and CCNA_01655 are separated by 91 nucleotides
CCNA_01651: CCNA_01651 - DNA gyrase subunit A, at 1,771,304 to 1,774,063
_01651
CCNA_01652: CCNA_01652 - phosphopantetheine adenylyltransferase, at 1,774,090 to 1,774,581
_01652
CCNA_01653: CCNA_01653 - cyclophilin-type peptidylprolyl cis-trans isomerase, at 1,774,739 to 1,775,572
_01653
CCNA_01654: CCNA_01654 - cyclophilin-type peptidylprolyl cis-trans isomerase, at 1,775,569 to 1,776,417
_01654
CCNA_01655: CCNA_01655 - cyclophilin-type peptidylprolyl cis-trans isomerase, at 1,776,509 to 1,776,976
_01655
Position (kb)
1774
1775
1776 Strain fitness (log2 ratio)
-2
-1
0
1 at 1773.991 kb on + strand at 1774.083 kb on + strand at 1774.084 kb on - strand at 1774.575 kb on + strand at 1774.576 kb on + strand at 1774.576 kb on + strand at 1774.576 kb on + strand at 1774.604 kb on + strand at 1774.715 kb on + strand at 1774.728 kb on - strand at 1774.799 kb on - strand at 1774.803 kb on + strand at 1774.813 kb on + strand at 1774.828 kb on + strand, within CCNA_01653 at 1774.840 kb on + strand, within CCNA_01653 at 1774.841 kb on - strand, within CCNA_01653 at 1774.891 kb on + strand, within CCNA_01653 at 1774.920 kb on - strand, within CCNA_01653 at 1775.027 kb on - strand, within CCNA_01653 at 1775.027 kb on - strand, within CCNA_01653 at 1775.051 kb on - strand, within CCNA_01653 at 1775.134 kb on + strand, within CCNA_01653 at 1775.163 kb on + strand, within CCNA_01653 at 1775.163 kb on + strand, within CCNA_01653 at 1775.234 kb on - strand, within CCNA_01653 at 1775.275 kb on + strand, within CCNA_01653 at 1775.275 kb on + strand, within CCNA_01653 at 1775.308 kb on + strand, within CCNA_01653 at 1775.322 kb on - strand, within CCNA_01653 at 1775.332 kb on - strand, within CCNA_01653 at 1775.339 kb on + strand, within CCNA_01653 at 1775.356 kb on + strand, within CCNA_01653 at 1775.356 kb on + strand, within CCNA_01653 at 1775.356 kb on + strand, within CCNA_01653 at 1775.364 kb on - strand, within CCNA_01653 at 1775.364 kb on - strand, within CCNA_01653 at 1775.364 kb on - strand, within CCNA_01653 at 1775.398 kb on + strand, within CCNA_01653 at 1775.406 kb on - strand, within CCNA_01653 at 1775.461 kb on + strand, within CCNA_01653 at 1775.493 kb on - strand at 1775.761 kb on - strand, within CCNA_01654 at 1775.805 kb on - strand, within CCNA_01654 at 1775.842 kb on + strand, within CCNA_01654 at 1775.849 kb on + strand, within CCNA_01654 at 1775.936 kb on + strand, within CCNA_01654 at 1775.936 kb on + strand, within CCNA_01654 at 1775.963 kb on + strand, within CCNA_01654 at 1775.963 kb on + strand, within CCNA_01654 at 1775.971 kb on - strand, within CCNA_01654 at 1775.978 kb on + strand, within CCNA_01654 at 1776.001 kb on + strand, within CCNA_01654 at 1776.043 kb on + strand, within CCNA_01654 at 1776.060 kb on + strand, within CCNA_01654 at 1776.067 kb on - strand, within CCNA_01654 at 1776.097 kb on + strand, within CCNA_01654 at 1776.146 kb on - strand, within CCNA_01654 at 1776.159 kb on + strand, within CCNA_01654 at 1776.204 kb on - strand, within CCNA_01654 at 1776.287 kb on - strand, within CCNA_01654 at 1776.350 kb on - strand at 1776.454 kb on - strand at 1776.476 kb on - strand at 1776.555 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with CHIR-090 10 ug/mL remove 1,773,991 + +0.6 1,774,083 + +1.6 1,774,084 - +0.0 1,774,575 + +0.2 1,774,576 + +0.1 1,774,576 + -1.1 1,774,576 + +0.9 1,774,604 + -0.8 1,774,715 + -0.1 1,774,728 - -1.1 1,774,799 - -0.4 1,774,803 + +0.0 1,774,813 + +0.9 1,774,828 + CCNA_01653 0.11 +0.3 1,774,840 + CCNA_01653 0.12 +0.6 1,774,841 - CCNA_01653 0.12 +0.3 1,774,891 + CCNA_01653 0.18 +0.3 1,774,920 - CCNA_01653 0.22 -1.9 1,775,027 - CCNA_01653 0.35 +1.1 1,775,027 - CCNA_01653 0.35 +1.0 1,775,051 - CCNA_01653 0.37 -0.4 1,775,134 + CCNA_01653 0.47 +0.1 1,775,163 + CCNA_01653 0.51 -0.1 1,775,163 + CCNA_01653 0.51 +1.1 1,775,234 - CCNA_01653 0.59 -0.9 1,775,275 + CCNA_01653 0.64 +0.3 1,775,275 + CCNA_01653 0.64 +1.2 1,775,308 + CCNA_01653 0.68 +0.4 1,775,322 - CCNA_01653 0.70 -0.2 1,775,332 - CCNA_01653 0.71 -0.9 1,775,339 + CCNA_01653 0.72 -0.4 1,775,356 + CCNA_01653 0.74 +0.8 1,775,356 + CCNA_01653 0.74 +1.1 1,775,356 + CCNA_01653 0.74 +0.5 1,775,364 - CCNA_01653 0.75 +0.7 1,775,364 - CCNA_01653 0.75 +0.6 1,775,364 - CCNA_01653 0.75 -1.3 1,775,398 + CCNA_01653 0.79 +0.3 1,775,406 - CCNA_01653 0.80 +0.7 1,775,461 + CCNA_01653 0.87 +0.6 1,775,493 - +1.5 1,775,761 - CCNA_01654 0.23 -1.5 1,775,805 - CCNA_01654 0.28 -0.2 1,775,842 + CCNA_01654 0.32 +0.4 1,775,849 + CCNA_01654 0.33 +0.9 1,775,936 + CCNA_01654 0.43 +1.0 1,775,936 + CCNA_01654 0.43 +0.6 1,775,963 + CCNA_01654 0.46 +0.4 1,775,963 + CCNA_01654 0.46 -0.5 1,775,971 - CCNA_01654 0.47 +0.9 1,775,978 + CCNA_01654 0.48 -0.1 1,776,001 + CCNA_01654 0.51 +0.2 1,776,043 + CCNA_01654 0.56 +0.2 1,776,060 + CCNA_01654 0.58 +0.3 1,776,067 - CCNA_01654 0.59 -0.4 1,776,097 + CCNA_01654 0.62 +0.6 1,776,146 - CCNA_01654 0.68 +0.7 1,776,159 + CCNA_01654 0.69 +0.2 1,776,204 - CCNA_01654 0.75 +0.6 1,776,287 - CCNA_01654 0.85 -0.2 1,776,350 - -2.3 1,776,454 - +0.8 1,776,476 - -0.6 1,776,555 + -0.1
Or see this region's nucleotide sequence