Experiment: PYE with CHIR-090 10 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_01580 and CCNA_01581 are separated by 108 nucleotides CCNA_01581 and CCNA_01582 are separated by 114 nucleotides CCNA_01582 and CCNA_01583 are separated by 69 nucleotides
CCNA_01580: CCNA_01580 - M15 superfamily membrane peptidase, at 1,696,883 to 1,697,533
_01580
CCNA_01581: CCNA_01581 - hypothetical protein, at 1,697,642 to 1,697,974
_01581
CCNA_01582: CCNA_01582 - Glutathione-dependent formaldehyde-activating enzyme, at 1,698,089 to 1,698,451
_01582
CCNA_01583: CCNA_01583 - phosphate-binding protein, at 1,698,521 to 1,699,561
_01583
CCNA_03954: CCNA_03954 - hypothetical protein, at 1,699,323 to 1,699,610
_03954
Position (kb)
1698
1699 Strain fitness (log2 ratio)
-2
-1
0
1 at 1697.133 kb on - strand, within CCNA_01580 at 1697.137 kb on + strand, within CCNA_01580 at 1697.177 kb on + strand, within CCNA_01580 at 1697.182 kb on + strand, within CCNA_01580 at 1697.190 kb on - strand, within CCNA_01580 at 1697.190 kb on - strand, within CCNA_01580 at 1697.238 kb on + strand, within CCNA_01580 at 1697.238 kb on + strand, within CCNA_01580 at 1697.238 kb on + strand, within CCNA_01580 at 1697.238 kb on + strand, within CCNA_01580 at 1697.238 kb on + strand, within CCNA_01580 at 1697.238 kb on + strand, within CCNA_01580 at 1697.246 kb on - strand, within CCNA_01580 at 1697.246 kb on - strand, within CCNA_01580 at 1697.270 kb on + strand, within CCNA_01580 at 1697.270 kb on + strand, within CCNA_01580 at 1697.278 kb on - strand, within CCNA_01580 at 1697.374 kb on - strand, within CCNA_01580 at 1697.416 kb on + strand, within CCNA_01580 at 1697.527 kb on + strand at 1697.535 kb on - strand at 1697.599 kb on - strand at 1697.604 kb on + strand at 1697.610 kb on + strand at 1697.644 kb on - strand at 1697.672 kb on - strand at 1697.717 kb on - strand, within CCNA_01581 at 1697.778 kb on - strand, within CCNA_01581 at 1697.862 kb on + strand, within CCNA_01581 at 1697.904 kb on - strand, within CCNA_01581 at 1697.973 kb on + strand at 1697.992 kb on + strand at 1698.000 kb on - strand at 1698.072 kb on + strand at 1698.125 kb on - strand at 1698.144 kb on - strand, within CCNA_01582 at 1698.188 kb on - strand, within CCNA_01582 at 1698.199 kb on + strand, within CCNA_01582 at 1698.204 kb on + strand, within CCNA_01582 at 1698.267 kb on + strand, within CCNA_01582 at 1698.337 kb on + strand, within CCNA_01582 at 1698.377 kb on - strand, within CCNA_01582 at 1698.416 kb on + strand at 1698.509 kb on - strand at 1698.519 kb on + strand at 1698.521 kb on - strand at 1698.522 kb on - strand at 1698.585 kb on + strand at 1698.800 kb on - strand, within CCNA_01583 at 1699.062 kb on + strand, within CCNA_01583
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with CHIR-090 10 ug/mL remove 1,697,133 - CCNA_01580 0.38 -0.2 1,697,137 + CCNA_01580 0.39 +0.5 1,697,177 + CCNA_01580 0.45 -0.0 1,697,182 + CCNA_01580 0.46 +0.4 1,697,190 - CCNA_01580 0.47 -0.8 1,697,190 - CCNA_01580 0.47 -0.5 1,697,238 + CCNA_01580 0.55 +0.7 1,697,238 + CCNA_01580 0.55 +0.1 1,697,238 + CCNA_01580 0.55 +0.9 1,697,238 + CCNA_01580 0.55 -1.7 1,697,238 + CCNA_01580 0.55 +0.3 1,697,238 + CCNA_01580 0.55 -0.2 1,697,246 - CCNA_01580 0.56 -1.2 1,697,246 - CCNA_01580 0.56 +0.1 1,697,270 + CCNA_01580 0.59 -1.2 1,697,270 + CCNA_01580 0.59 +0.7 1,697,278 - CCNA_01580 0.61 -1.3 1,697,374 - CCNA_01580 0.75 -1.2 1,697,416 + CCNA_01580 0.82 +0.3 1,697,527 + +0.9 1,697,535 - -0.3 1,697,599 - -0.7 1,697,604 + +0.8 1,697,610 + +0.1 1,697,644 - -1.1 1,697,672 - -0.7 1,697,717 - CCNA_01581 0.23 +0.1 1,697,778 - CCNA_01581 0.41 +0.5 1,697,862 + CCNA_01581 0.66 +0.7 1,697,904 - CCNA_01581 0.79 +0.3 1,697,973 + +0.4 1,697,992 + -0.6 1,698,000 - +0.1 1,698,072 + +0.1 1,698,125 - -1.8 1,698,144 - CCNA_01582 0.15 +1.5 1,698,188 - CCNA_01582 0.27 -0.9 1,698,199 + CCNA_01582 0.30 +0.2 1,698,204 + CCNA_01582 0.32 +0.4 1,698,267 + CCNA_01582 0.49 -0.3 1,698,337 + CCNA_01582 0.68 +0.4 1,698,377 - CCNA_01582 0.79 +0.2 1,698,416 + +0.9 1,698,509 - +1.3 1,698,519 + -0.8 1,698,521 - +0.4 1,698,522 - -1.2 1,698,585 + +0.3 1,698,800 - CCNA_01583 0.27 -1.1 1,699,062 + CCNA_01583 0.52 +1.6
Or see this region's nucleotide sequence