Experiment: PYE with CHIR-090 10 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_01432 and CCNA_01433 are separated by 214 nucleotides CCNA_01433 and CCNA_01434 are separated by 12 nucleotides CCNA_01434 and CCNA_R0035 are separated by 63 nucleotides CCNA_R0035 and CCNA_01435 are separated by 65 nucleotides CCNA_01435 and CCNA_01437 overlap by 4 nucleotides CCNA_01437 and CCNA_01438 are separated by 150 nucleotides
CCNA_01432: CCNA_01432 - 3-oxoacyl-(acyl-carrier-protein) synthase III, at 1,547,371 to 1,548,348
_01432
CCNA_01433: CCNA_01433 - integration host factor alpha-subunit, at 1,548,563 to 1,548,865
_01433
CCNA_01434: CCNA_01434 - MerR-family transcriptional regulator, at 1,548,878 to 1,549,303
_01434
CCNA_R0035: CCNA_R0035 - tRNA-Pro, at 1,549,367 to 1,549,443
_R0035
CCNA_01435: CCNA_01435 - hypothetical protein, at 1,549,509 to 1,549,760
_01435
CCNA_01437: CCNA_01437 - transglycosylase associated protein, at 1,549,757 to 1,550,008
_01437
CCNA_01438: CCNA_01438 - heme O monooxygenase, at 1,550,159 to 1,551,190
_01438
Position (kb)
1548
1549
1550 Strain fitness (log2 ratio)
-2
-1
0
1 at 1548.382 kb on - strand at 1548.399 kb on + strand at 1548.420 kb on - strand at 1548.456 kb on + strand at 1548.478 kb on - strand at 1548.575 kb on + strand at 1548.936 kb on - strand, within CCNA_01434 at 1548.936 kb on - strand, within CCNA_01434 at 1549.032 kb on + strand, within CCNA_01434 at 1549.040 kb on - strand, within CCNA_01434 at 1549.045 kb on - strand, within CCNA_01434 at 1549.056 kb on + strand, within CCNA_01434 at 1549.058 kb on + strand, within CCNA_01434 at 1549.058 kb on + strand, within CCNA_01434 at 1549.058 kb on + strand, within CCNA_01434 at 1549.066 kb on - strand, within CCNA_01434 at 1549.068 kb on + strand, within CCNA_01434 at 1549.068 kb on + strand, within CCNA_01434 at 1549.068 kb on + strand, within CCNA_01434 at 1549.076 kb on - strand, within CCNA_01434 at 1549.081 kb on + strand, within CCNA_01434 at 1549.089 kb on - strand, within CCNA_01434 at 1549.175 kb on + strand, within CCNA_01434 at 1549.175 kb on + strand, within CCNA_01434 at 1549.175 kb on + strand, within CCNA_01434 at 1549.183 kb on - strand, within CCNA_01434 at 1549.231 kb on - strand, within CCNA_01434 at 1549.299 kb on + strand at 1549.486 kb on - strand at 1549.524 kb on + strand at 1549.532 kb on - strand at 1549.560 kb on - strand, within CCNA_01435 at 1549.577 kb on + strand, within CCNA_01435 at 1549.587 kb on - strand, within CCNA_01435 at 1549.626 kb on + strand, within CCNA_01435 at 1549.626 kb on + strand, within CCNA_01435 at 1549.629 kb on - strand, within CCNA_01435 at 1549.634 kb on - strand, within CCNA_01435 at 1549.649 kb on + strand, within CCNA_01435 at 1549.657 kb on - strand, within CCNA_01435 at 1549.660 kb on + strand, within CCNA_01435 at 1549.660 kb on + strand, within CCNA_01435 at 1549.685 kb on + strand, within CCNA_01435 at 1549.685 kb on + strand, within CCNA_01435 at 1549.728 kb on + strand, within CCNA_01435 at 1549.777 kb on + strand at 1549.785 kb on - strand, within CCNA_01437 at 1549.785 kb on - strand, within CCNA_01437 at 1549.785 kb on - strand, within CCNA_01437 at 1549.788 kb on + strand, within CCNA_01437 at 1549.846 kb on + strand, within CCNA_01437 at 1549.854 kb on - strand, within CCNA_01437 at 1549.854 kb on - strand, within CCNA_01437 at 1549.868 kb on + strand, within CCNA_01437 at 1549.874 kb on - strand, within CCNA_01437 at 1549.909 kb on - strand, within CCNA_01437 at 1549.921 kb on - strand, within CCNA_01437 at 1549.942 kb on - strand, within CCNA_01437 at 1549.974 kb on - strand, within CCNA_01437 at 1550.048 kb on - strand at 1550.058 kb on + strand at 1550.122 kb on + strand at 1550.228 kb on + strand at 1550.230 kb on - strand at 1550.236 kb on - strand at 1550.271 kb on + strand, within CCNA_01438 at 1550.289 kb on - strand, within CCNA_01438 at 1550.303 kb on + strand, within CCNA_01438
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with CHIR-090 10 ug/mL remove 1,548,382 - +0.8 1,548,399 + -0.9 1,548,420 - -0.7 1,548,456 + +0.6 1,548,478 - +0.4 1,548,575 + -0.6 1,548,936 - CCNA_01434 0.14 -0.4 1,548,936 - CCNA_01434 0.14 -0.1 1,549,032 + CCNA_01434 0.36 -0.2 1,549,040 - CCNA_01434 0.38 +0.3 1,549,045 - CCNA_01434 0.39 -0.3 1,549,056 + CCNA_01434 0.42 +0.4 1,549,058 + CCNA_01434 0.42 +0.7 1,549,058 + CCNA_01434 0.42 +0.3 1,549,058 + CCNA_01434 0.42 +0.9 1,549,066 - CCNA_01434 0.44 -1.2 1,549,068 + CCNA_01434 0.45 +0.8 1,549,068 + CCNA_01434 0.45 +0.5 1,549,068 + CCNA_01434 0.45 +0.2 1,549,076 - CCNA_01434 0.46 +0.9 1,549,081 + CCNA_01434 0.48 +0.5 1,549,089 - CCNA_01434 0.50 +0.3 1,549,175 + CCNA_01434 0.70 +0.8 1,549,175 + CCNA_01434 0.70 +0.4 1,549,175 + CCNA_01434 0.70 +0.7 1,549,183 - CCNA_01434 0.72 +0.7 1,549,231 - CCNA_01434 0.83 -1.3 1,549,299 + -0.2 1,549,486 - -0.5 1,549,524 + -0.4 1,549,532 - -0.8 1,549,560 - CCNA_01435 0.20 -1.4 1,549,577 + CCNA_01435 0.27 -1.0 1,549,587 - CCNA_01435 0.31 +0.7 1,549,626 + CCNA_01435 0.46 -0.2 1,549,626 + CCNA_01435 0.46 -2.0 1,549,629 - CCNA_01435 0.48 +0.0 1,549,634 - CCNA_01435 0.50 +0.2 1,549,649 + CCNA_01435 0.56 -0.3 1,549,657 - CCNA_01435 0.59 +0.1 1,549,660 + CCNA_01435 0.60 -0.3 1,549,660 + CCNA_01435 0.60 +1.7 1,549,685 + CCNA_01435 0.70 -2.4 1,549,685 + CCNA_01435 0.70 -0.7 1,549,728 + CCNA_01435 0.87 -2.7 1,549,777 + +0.2 1,549,785 - CCNA_01437 0.11 -0.7 1,549,785 - CCNA_01437 0.11 +0.2 1,549,785 - CCNA_01437 0.11 -0.1 1,549,788 + CCNA_01437 0.12 -0.2 1,549,846 + CCNA_01437 0.35 -2.3 1,549,854 - CCNA_01437 0.38 +0.4 1,549,854 - CCNA_01437 0.38 -1.3 1,549,868 + CCNA_01437 0.44 -0.2 1,549,874 - CCNA_01437 0.46 -0.1 1,549,909 - CCNA_01437 0.60 -0.7 1,549,921 - CCNA_01437 0.65 +0.7 1,549,942 - CCNA_01437 0.73 +0.2 1,549,974 - CCNA_01437 0.86 -0.8 1,550,048 - +0.0 1,550,058 + -0.6 1,550,122 + +1.0 1,550,228 + +0.5 1,550,230 - -0.2 1,550,236 - -0.5 1,550,271 + CCNA_01438 0.11 -1.0 1,550,289 - CCNA_01438 0.13 -2.3 1,550,303 + CCNA_01438 0.14 +0.2
Or see this region's nucleotide sequence