Experiment: PYE with CHIR-090 10 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_01419 and CCNA_01420 are separated by 32 nucleotides CCNA_01420 and CCNA_01421 are separated by 65 nucleotides CCNA_01421 and CCNA_01422 overlap by 4 nucleotides CCNA_01422 and CCNA_01423 overlap by 41 nucleotides
CCNA_01419: CCNA_01419 - serine hydroxymethyltransferase, at 1,536,723 to 1,538,009
_01419
CCNA_01420: CCNA_01420 - NrdR family transcriptional regulator, at 1,538,042 to 1,538,515
_01420
CCNA_01421: CCNA_01421 - 6,7-dimethyl-8-ribityllumazine synthase, at 1,538,581 to 1,539,045
_01421
CCNA_01422: CCNA_01422 - N utilization substance protein B NusB, at 1,539,042 to 1,539,491
_01422
CCNA_01423: CCNA_01423 - thiamine-monophosphate kinase, at 1,539,451 to 1,540,509
_01423
Position (kb)
1538
1539 Strain fitness (log2 ratio)
-2
-1
0
1 at 1537.055 kb on + strand, within CCNA_01419 at 1537.063 kb on - strand, within CCNA_01419 at 1537.164 kb on - strand, within CCNA_01419 at 1537.191 kb on + strand, within CCNA_01419 at 1537.247 kb on + strand, within CCNA_01419 at 1537.354 kb on + strand, within CCNA_01419 at 1537.354 kb on + strand, within CCNA_01419 at 1537.400 kb on + strand, within CCNA_01419 at 1537.458 kb on + strand, within CCNA_01419 at 1537.479 kb on + strand, within CCNA_01419 at 1537.580 kb on - strand, within CCNA_01419 at 1537.629 kb on - strand, within CCNA_01419 at 1537.631 kb on + strand, within CCNA_01419 at 1537.734 kb on + strand, within CCNA_01419 at 1537.739 kb on + strand, within CCNA_01419 at 1537.742 kb on - strand, within CCNA_01419 at 1537.747 kb on - strand, within CCNA_01419 at 1537.787 kb on + strand, within CCNA_01419 at 1537.795 kb on - strand, within CCNA_01419 at 1537.795 kb on - strand, within CCNA_01419 at 1537.855 kb on + strand, within CCNA_01419 at 1537.863 kb on - strand, within CCNA_01419 at 1537.902 kb on + strand at 1537.910 kb on - strand at 1537.984 kb on - strand at 1538.132 kb on + strand, within CCNA_01420 at 1538.133 kb on + strand, within CCNA_01420 at 1538.161 kb on + strand, within CCNA_01420 at 1538.242 kb on + strand, within CCNA_01420 at 1538.261 kb on + strand, within CCNA_01420 at 1538.424 kb on + strand, within CCNA_01420 at 1538.467 kb on + strand, within CCNA_01420 at 1538.491 kb on - strand at 1538.517 kb on - strand at 1538.542 kb on - strand at 1538.577 kb on - strand at 1539.513 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with CHIR-090 10 ug/mL remove 1,537,055 + CCNA_01419 0.26 +0.9 1,537,063 - CCNA_01419 0.26 -0.2 1,537,164 - CCNA_01419 0.34 +0.9 1,537,191 + CCNA_01419 0.36 +0.6 1,537,247 + CCNA_01419 0.41 +0.5 1,537,354 + CCNA_01419 0.49 +0.7 1,537,354 + CCNA_01419 0.49 +0.7 1,537,400 + CCNA_01419 0.53 +0.3 1,537,458 + CCNA_01419 0.57 +0.2 1,537,479 + CCNA_01419 0.59 -0.0 1,537,580 - CCNA_01419 0.67 -0.1 1,537,629 - CCNA_01419 0.70 -1.5 1,537,631 + CCNA_01419 0.71 +0.3 1,537,734 + CCNA_01419 0.79 +0.1 1,537,739 + CCNA_01419 0.79 +0.5 1,537,742 - CCNA_01419 0.79 +0.2 1,537,747 - CCNA_01419 0.80 -0.5 1,537,787 + CCNA_01419 0.83 +0.4 1,537,795 - CCNA_01419 0.83 -0.4 1,537,795 - CCNA_01419 0.83 -0.4 1,537,855 + CCNA_01419 0.88 +0.5 1,537,863 - CCNA_01419 0.89 -2.0 1,537,902 + +0.9 1,537,910 - -0.5 1,537,984 - -0.0 1,538,132 + CCNA_01420 0.19 -0.3 1,538,133 + CCNA_01420 0.19 -0.3 1,538,161 + CCNA_01420 0.25 +0.9 1,538,242 + CCNA_01420 0.42 +0.4 1,538,261 + CCNA_01420 0.46 +0.5 1,538,424 + CCNA_01420 0.81 +0.4 1,538,467 + CCNA_01420 0.90 +1.7 1,538,491 - -0.7 1,538,517 - -0.4 1,538,542 - +0.5 1,538,577 - -0.8 1,539,513 + -0.7
Or see this region's nucleotide sequence