Experiment: PYE with CHIR-090 10 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_00998 and CCNA_00999 are separated by 197 nucleotides CCNA_00999 and CCNA_01000 are separated by 55 nucleotides CCNA_01000 and CCNA_01001 are separated by 303 nucleotides
CCNA_00998: CCNA_00998 - ROS/MUCR transcriptional regulator, at 1,081,367 to 1,081,786
_00998
CCNA_00999: CCNA_00999 - PAS-family motility and cell cycle regulatory protein MopJ, at 1,081,984 to 1,082,481
_00999
CCNA_01000: CCNA_01000 - hypothetical protein, at 1,082,537 to 1,082,725
_01000
CCNA_01001: CCNA_01001 - ribokinase, at 1,083,029 to 1,083,940
_01001
Position (kb)
1082
1083 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1081.609 kb on + strand, within CCNA_00998 at 1081.614 kb on + strand, within CCNA_00998 at 1081.622 kb on - strand, within CCNA_00998 at 1081.675 kb on + strand, within CCNA_00998 at 1081.675 kb on + strand, within CCNA_00998 at 1081.675 kb on + strand, within CCNA_00998 at 1081.675 kb on + strand, within CCNA_00998 at 1081.681 kb on + strand, within CCNA_00998 at 1081.683 kb on - strand, within CCNA_00998 at 1081.683 kb on - strand, within CCNA_00998 at 1081.689 kb on - strand, within CCNA_00998 at 1081.694 kb on - strand, within CCNA_00998 at 1081.726 kb on - strand, within CCNA_00998 at 1081.735 kb on + strand, within CCNA_00998 at 1081.843 kb on + strand at 1081.987 kb on - strand at 1082.040 kb on - strand, within CCNA_00999 at 1082.132 kb on + strand, within CCNA_00999 at 1082.151 kb on + strand, within CCNA_00999 at 1082.179 kb on - strand, within CCNA_00999 at 1082.199 kb on + strand, within CCNA_00999 at 1082.258 kb on - strand, within CCNA_00999 at 1082.337 kb on + strand, within CCNA_00999 at 1082.350 kb on + strand, within CCNA_00999 at 1082.355 kb on + strand, within CCNA_00999 at 1082.358 kb on + strand, within CCNA_00999 at 1082.368 kb on - strand, within CCNA_00999 at 1082.457 kb on - strand at 1082.559 kb on - strand, within CCNA_01000 at 1082.582 kb on - strand, within CCNA_01000 at 1082.582 kb on - strand, within CCNA_01000 at 1082.582 kb on - strand, within CCNA_01000 at 1082.582 kb on - strand, within CCNA_01000 at 1082.582 kb on - strand, within CCNA_01000 at 1082.712 kb on + strand at 1082.732 kb on - strand at 1082.770 kb on + strand at 1082.770 kb on + strand at 1082.770 kb on + strand at 1082.778 kb on - strand at 1082.778 kb on - strand at 1082.778 kb on - strand at 1082.785 kb on + strand at 1082.785 kb on + strand at 1082.800 kb on - strand at 1082.955 kb on + strand at 1082.974 kb on + strand at 1082.983 kb on - strand at 1083.000 kb on + strand at 1083.107 kb on - strand at 1083.213 kb on + strand, within CCNA_01001 at 1083.299 kb on - strand, within CCNA_01001 at 1083.304 kb on - strand, within CCNA_01001 at 1083.309 kb on - strand, within CCNA_01001 at 1083.319 kb on - strand, within CCNA_01001 at 1083.358 kb on + strand, within CCNA_01001 at 1083.369 kb on + strand, within CCNA_01001 at 1083.377 kb on - strand, within CCNA_01001 at 1083.388 kb on + strand, within CCNA_01001 at 1083.438 kb on + strand, within CCNA_01001 at 1083.438 kb on + strand, within CCNA_01001 at 1083.446 kb on - strand, within CCNA_01001 at 1083.547 kb on + strand, within CCNA_01001 at 1083.594 kb on + strand, within CCNA_01001 at 1083.629 kb on - strand, within CCNA_01001 at 1083.641 kb on + strand, within CCNA_01001 at 1083.654 kb on + strand, within CCNA_01001 at 1083.656 kb on - strand, within CCNA_01001 at 1083.667 kb on - strand, within CCNA_01001 at 1083.667 kb on - strand, within CCNA_01001 at 1083.667 kb on - strand, within CCNA_01001 at 1083.667 kb on - strand, within CCNA_01001 at 1083.709 kb on - strand, within CCNA_01001 at 1083.717 kb on - strand, within CCNA_01001
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with CHIR-090 10 ug/mL remove 1,081,609 + CCNA_00998 0.58 +0.7 1,081,614 + CCNA_00998 0.59 -0.9 1,081,622 - CCNA_00998 0.61 -0.7 1,081,675 + CCNA_00998 0.73 +0.1 1,081,675 + CCNA_00998 0.73 +0.5 1,081,675 + CCNA_00998 0.73 -0.3 1,081,675 + CCNA_00998 0.73 +0.6 1,081,681 + CCNA_00998 0.75 -0.3 1,081,683 - CCNA_00998 0.75 +0.1 1,081,683 - CCNA_00998 0.75 -1.2 1,081,689 - CCNA_00998 0.77 +0.8 1,081,694 - CCNA_00998 0.78 -1.0 1,081,726 - CCNA_00998 0.85 -0.2 1,081,735 + CCNA_00998 0.88 +1.3 1,081,843 + +0.8 1,081,987 - +0.5 1,082,040 - CCNA_00999 0.11 -1.9 1,082,132 + CCNA_00999 0.30 -0.1 1,082,151 + CCNA_00999 0.34 -0.0 1,082,179 - CCNA_00999 0.39 +0.2 1,082,199 + CCNA_00999 0.43 -1.1 1,082,258 - CCNA_00999 0.55 -0.4 1,082,337 + CCNA_00999 0.71 -0.4 1,082,350 + CCNA_00999 0.73 +1.0 1,082,355 + CCNA_00999 0.74 +0.6 1,082,358 + CCNA_00999 0.75 -0.9 1,082,368 - CCNA_00999 0.77 +0.2 1,082,457 - -0.2 1,082,559 - CCNA_01000 0.12 +0.1 1,082,582 - CCNA_01000 0.24 +1.2 1,082,582 - CCNA_01000 0.24 +0.6 1,082,582 - CCNA_01000 0.24 -0.2 1,082,582 - CCNA_01000 0.24 +1.0 1,082,582 - CCNA_01000 0.24 +0.2 1,082,712 + +0.6 1,082,732 - +1.4 1,082,770 + +1.0 1,082,770 + -0.0 1,082,770 + +0.5 1,082,778 - +0.4 1,082,778 - +0.2 1,082,778 - +0.6 1,082,785 + +0.2 1,082,785 + +1.8 1,082,800 - +0.6 1,082,955 + -0.7 1,082,974 + -0.3 1,082,983 - +0.2 1,083,000 + -0.7 1,083,107 - -0.4 1,083,213 + CCNA_01001 0.20 +1.1 1,083,299 - CCNA_01001 0.30 -0.3 1,083,304 - CCNA_01001 0.30 -0.9 1,083,309 - CCNA_01001 0.31 -1.5 1,083,319 - CCNA_01001 0.32 +0.3 1,083,358 + CCNA_01001 0.36 -0.6 1,083,369 + CCNA_01001 0.37 +0.6 1,083,377 - CCNA_01001 0.38 -0.3 1,083,388 + CCNA_01001 0.39 +0.3 1,083,438 + CCNA_01001 0.45 +0.1 1,083,438 + CCNA_01001 0.45 -0.9 1,083,446 - CCNA_01001 0.46 +1.6 1,083,547 + CCNA_01001 0.57 -0.8 1,083,594 + CCNA_01001 0.62 +0.3 1,083,629 - CCNA_01001 0.66 +1.0 1,083,641 + CCNA_01001 0.67 -1.2 1,083,654 + CCNA_01001 0.69 -0.7 1,083,656 - CCNA_01001 0.69 -0.0 1,083,667 - CCNA_01001 0.70 +0.7 1,083,667 - CCNA_01001 0.70 -1.1 1,083,667 - CCNA_01001 0.70 +0.3 1,083,667 - CCNA_01001 0.70 -1.1 1,083,709 - CCNA_01001 0.75 -0.3 1,083,717 - CCNA_01001 0.75 -0.9
Or see this region's nucleotide sequence