Experiment: PYE with CHIR-090 10 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_00747 and CCNA_00748 are separated by 210 nucleotides CCNA_00748 and CCNA_00749 are separated by 4 nucleotides CCNA_00749 and CCNA_00750 are separated by 208 nucleotides
CCNA_00747: CCNA_00747 - cyclic-di-GMP phosphodiesterase, flagellum assembly factor TipF, at 803,595 to 804,953
_00747
CCNA_00748: CCNA_00748 - ferrous iron transport protein A, at 805,164 to 805,484
_00748
CCNA_00749: CCNA_00749 - ferrous iron transport protein B, at 805,489 to 807,384
_00749
CCNA_00750: CCNA_00750 - FeaR-family transcriptional regulator, at 807,593 to 808,621
_00750
Position (kb) 805
806
807
808 Strain fitness (log2 ratio) -2
-1
0
1
2 at 804.544 kb on + strand, within CCNA_00747 at 804.544 kb on + strand, within CCNA_00747 at 804.557 kb on - strand, within CCNA_00747 at 804.565 kb on - strand, within CCNA_00747 at 804.691 kb on - strand, within CCNA_00747 at 804.729 kb on + strand, within CCNA_00747 at 804.742 kb on - strand, within CCNA_00747 at 804.747 kb on + strand, within CCNA_00747 at 804.840 kb on - strand at 804.840 kb on - strand at 804.890 kb on + strand at 804.900 kb on + strand at 804.909 kb on - strand at 804.909 kb on - strand at 804.932 kb on + strand at 804.932 kb on - strand at 804.932 kb on - strand at 804.971 kb on + strand at 804.976 kb on + strand at 804.976 kb on + strand at 804.976 kb on + strand at 804.976 kb on + strand at 804.979 kb on - strand at 804.979 kb on - strand at 804.981 kb on + strand at 804.981 kb on + strand at 804.989 kb on - strand at 804.989 kb on - strand at 805.043 kb on - strand at 805.049 kb on + strand at 805.098 kb on + strand at 805.164 kb on - strand at 805.166 kb on - strand at 805.479 kb on + strand at 805.479 kb on + strand at 805.487 kb on - strand at 806.016 kb on + strand, within CCNA_00749 at 806.078 kb on - strand, within CCNA_00749 at 807.374 kb on + strand at 807.383 kb on + strand at 807.404 kb on - strand at 807.427 kb on - strand at 807.483 kb on - strand at 807.494 kb on + strand at 807.505 kb on + strand at 807.505 kb on + strand at 807.515 kb on + strand at 807.540 kb on - strand at 807.571 kb on - strand at 807.604 kb on - strand at 807.632 kb on - strand at 807.635 kb on + strand at 807.635 kb on + strand at 807.643 kb on - strand at 807.643 kb on - strand at 807.643 kb on - strand at 807.643 kb on - strand at 807.643 kb on - strand at 807.733 kb on - strand, within CCNA_00750 at 807.744 kb on + strand, within CCNA_00750 at 807.777 kb on + strand, within CCNA_00750 at 807.824 kb on - strand, within CCNA_00750 at 807.905 kb on - strand, within CCNA_00750 at 807.978 kb on + strand, within CCNA_00750 at 807.997 kb on + strand, within CCNA_00750 at 808.049 kb on - strand, within CCNA_00750 at 808.049 kb on - strand, within CCNA_00750 at 808.086 kb on + strand, within CCNA_00750 at 808.107 kb on - strand, within CCNA_00750 at 808.107 kb on - strand, within CCNA_00750 at 808.109 kb on + strand, within CCNA_00750 at 808.117 kb on - strand, within CCNA_00750 at 808.117 kb on - strand, within CCNA_00750 at 808.167 kb on - strand, within CCNA_00750 at 808.230 kb on - strand, within CCNA_00750 at 808.292 kb on + strand, within CCNA_00750 at 808.347 kb on + strand, within CCNA_00750 at 808.356 kb on + strand, within CCNA_00750
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with CHIR-090 10 ug/mL remove 804,544 + CCNA_00747 0.70 +1.0 804,544 + CCNA_00747 0.70 -0.1 804,557 - CCNA_00747 0.71 -1.3 804,565 - CCNA_00747 0.71 +0.6 804,691 - CCNA_00747 0.81 -0.6 804,729 + CCNA_00747 0.83 -0.2 804,742 - CCNA_00747 0.84 +0.4 804,747 + CCNA_00747 0.85 -0.4 804,840 - -0.4 804,840 - +0.8 804,890 + -1.4 804,900 + -0.4 804,909 - +0.4 804,909 - +0.7 804,932 + +0.9 804,932 - +0.1 804,932 - +0.6 804,971 + +0.7 804,976 + +0.0 804,976 + +1.2 804,976 + +0.2 804,976 + -0.3 804,979 - +0.8 804,979 - +0.1 804,981 + -0.9 804,981 + +1.8 804,989 - +0.9 804,989 - +1.3 805,043 - +0.9 805,049 + +0.4 805,098 + +0.7 805,164 - +0.1 805,166 - +0.4 805,479 + +0.9 805,479 + +1.0 805,487 - +0.1 806,016 + CCNA_00749 0.28 +0.8 806,078 - CCNA_00749 0.31 -0.2 807,374 + +0.6 807,383 + +0.8 807,404 - -1.2 807,427 - +0.2 807,483 - +0.3 807,494 + +0.6 807,505 + +0.4 807,505 + +0.4 807,515 + +0.1 807,540 - -0.1 807,571 - -0.2 807,604 - +1.1 807,632 - +0.0 807,635 + +0.8 807,635 + +0.2 807,643 - +0.3 807,643 - +0.5 807,643 - +0.3 807,643 - -0.2 807,643 - +0.2 807,733 - CCNA_00750 0.14 +0.3 807,744 + CCNA_00750 0.15 -1.9 807,777 + CCNA_00750 0.18 -0.8 807,824 - CCNA_00750 0.22 +0.4 807,905 - CCNA_00750 0.30 +0.2 807,978 + CCNA_00750 0.37 +0.3 807,997 + CCNA_00750 0.39 +0.1 808,049 - CCNA_00750 0.44 -0.3 808,049 - CCNA_00750 0.44 +0.4 808,086 + CCNA_00750 0.48 +0.2 808,107 - CCNA_00750 0.50 +1.0 808,107 - CCNA_00750 0.50 +0.4 808,109 + CCNA_00750 0.50 +0.7 808,117 - CCNA_00750 0.51 +0.4 808,117 - CCNA_00750 0.51 +0.2 808,167 - CCNA_00750 0.56 -0.6 808,230 - CCNA_00750 0.62 +0.1 808,292 + CCNA_00750 0.68 +0.1 808,347 + CCNA_00750 0.73 +0.1 808,356 + CCNA_00750 0.74 +0.3
Or see this region's nucleotide sequence