Experiment: PYE with CHIR-090 10 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_00492 and CCNA_00493 are separated by 2 nucleotides CCNA_00493 and CCNA_00494 are separated by 6 nucleotides CCNA_00494 and CCNA_00495 are separated by 52 nucleotides CCNA_00495 and CCNA_00496 overlap by 4 nucleotides
CCNA_00492: CCNA_00492 - cysteinyl-tRNA synthetase, at 509,704 to 511,095
_00492
CCNA_00493: CCNA_00493 - putative polyketide cyclase-dehydrase/lipid transport protein, at 511,098 to 511,559
_00493
CCNA_00494: CCNA_00494 - transport protein, at 511,566 to 512,027
_00494
CCNA_00495: CCNA_00495 - putative membrane protein insertion efficiency factor YidD, at 512,080 to 512,334
_00495
CCNA_00496: CCNA_00496 - threonyl-tRNA synthetase, at 512,331 to 514,298
_00496
Position (kb)
512
513 Strain fitness (log2 ratio)
-2
-1
0
1 at 511.126 kb on - strand at 511.185 kb on + strand, within CCNA_00493 at 511.219 kb on + strand, within CCNA_00493 at 511.231 kb on + strand, within CCNA_00493 at 511.231 kb on + strand, within CCNA_00493 at 511.231 kb on + strand, within CCNA_00493 at 511.231 kb on + strand, within CCNA_00493 at 511.239 kb on - strand, within CCNA_00493 at 511.284 kb on - strand, within CCNA_00493 at 511.293 kb on + strand, within CCNA_00493 at 511.293 kb on + strand, within CCNA_00493 at 511.298 kb on + strand, within CCNA_00493 at 511.298 kb on + strand, within CCNA_00493 at 511.301 kb on - strand, within CCNA_00493 at 511.322 kb on - strand, within CCNA_00493 at 511.383 kb on + strand, within CCNA_00493 at 511.424 kb on + strand, within CCNA_00493 at 511.424 kb on + strand, within CCNA_00493 at 511.424 kb on + strand, within CCNA_00493 at 511.424 kb on + strand, within CCNA_00493 at 511.424 kb on + strand, within CCNA_00493 at 511.424 kb on + strand, within CCNA_00493 at 511.432 kb on - strand, within CCNA_00493 at 511.445 kb on - strand, within CCNA_00493 at 511.447 kb on - strand, within CCNA_00493 at 511.490 kb on - strand, within CCNA_00493 at 511.496 kb on + strand, within CCNA_00493 at 511.522 kb on + strand at 511.577 kb on + strand at 511.577 kb on + strand at 511.577 kb on + strand at 511.577 kb on + strand at 511.577 kb on + strand at 511.585 kb on - strand at 511.992 kb on + strand at 512.003 kb on + strand at 512.011 kb on - strand at 512.037 kb on + strand at 512.055 kb on - strand at 512.085 kb on - strand at 512.087 kb on + strand at 512.099 kb on + strand at 512.107 kb on - strand, within CCNA_00495 at 512.109 kb on + strand, within CCNA_00495 at 512.117 kb on - strand, within CCNA_00495 at 512.145 kb on + strand, within CCNA_00495 at 512.162 kb on + strand, within CCNA_00495 at 512.167 kb on + strand, within CCNA_00495 at 512.170 kb on - strand, within CCNA_00495 at 512.170 kb on - strand, within CCNA_00495 at 512.177 kb on + strand, within CCNA_00495 at 512.205 kb on + strand, within CCNA_00495 at 512.225 kb on - strand, within CCNA_00495 at 512.231 kb on + strand, within CCNA_00495 at 512.239 kb on - strand, within CCNA_00495 at 512.278 kb on + strand, within CCNA_00495 at 512.279 kb on + strand, within CCNA_00495
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with CHIR-090 10 ug/mL remove 511,126 - +0.8 511,185 + CCNA_00493 0.19 +0.2 511,219 + CCNA_00493 0.26 -0.0 511,231 + CCNA_00493 0.29 -0.6 511,231 + CCNA_00493 0.29 -0.1 511,231 + CCNA_00493 0.29 -0.4 511,231 + CCNA_00493 0.29 -0.5 511,239 - CCNA_00493 0.31 +0.6 511,284 - CCNA_00493 0.40 -1.3 511,293 + CCNA_00493 0.42 +0.2 511,293 + CCNA_00493 0.42 -0.6 511,298 + CCNA_00493 0.43 +0.3 511,298 + CCNA_00493 0.43 +0.8 511,301 - CCNA_00493 0.44 +1.3 511,322 - CCNA_00493 0.48 -0.9 511,383 + CCNA_00493 0.62 -0.2 511,424 + CCNA_00493 0.71 +0.4 511,424 + CCNA_00493 0.71 -0.6 511,424 + CCNA_00493 0.71 +0.3 511,424 + CCNA_00493 0.71 +0.4 511,424 + CCNA_00493 0.71 +0.9 511,424 + CCNA_00493 0.71 -0.3 511,432 - CCNA_00493 0.72 -1.1 511,445 - CCNA_00493 0.75 -1.3 511,447 - CCNA_00493 0.76 +0.5 511,490 - CCNA_00493 0.85 -0.3 511,496 + CCNA_00493 0.86 +1.4 511,522 + -2.2 511,577 + -0.2 511,577 + -1.3 511,577 + +0.5 511,577 + -0.2 511,577 + -0.4 511,585 - -1.4 511,992 + -0.2 512,003 + -0.6 512,011 - +0.5 512,037 + +1.0 512,055 - -1.1 512,085 - -0.6 512,087 + +0.5 512,099 + +0.8 512,107 - CCNA_00495 0.11 -1.7 512,109 + CCNA_00495 0.11 +0.5 512,117 - CCNA_00495 0.15 -1.3 512,145 + CCNA_00495 0.25 +0.8 512,162 + CCNA_00495 0.32 -0.2 512,167 + CCNA_00495 0.34 +0.1 512,170 - CCNA_00495 0.35 +0.5 512,170 - CCNA_00495 0.35 +0.6 512,177 + CCNA_00495 0.38 -0.1 512,205 + CCNA_00495 0.49 +0.0 512,225 - CCNA_00495 0.57 -0.6 512,231 + CCNA_00495 0.59 +0.1 512,239 - CCNA_00495 0.62 +0.8 512,278 + CCNA_00495 0.78 -0.3 512,279 + CCNA_00495 0.78 -0.2
Or see this region's nucleotide sequence