Experiment: PYE with CHIR-090 10 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_00470 and CCNA_00471 are separated by 26 nucleotides CCNA_00471 and CCNA_00472 overlap by 35 nucleotides CCNA_00472 and CCNA_00473 are separated by 104 nucleotides CCNA_00473 and CCNA_00474 overlap by 1 nucleotides
CCNA_00470: CCNA_00470 - O-antigen polymerase, at 484,789 to 486,132
_00470
CCNA_00471: CCNA_00471 - GDP-L-fucose synthase, at 486,159 to 487,127
_00471
CCNA_00472: CCNA_00472 - GDP-mannose 4,6 dehydratase, at 487,093 to 488,169
_00472
CCNA_00473: CCNA_00473 - hypothetical protein, at 488,274 to 488,768
_00473
CCNA_00474: CCNA_00474 - DNA relaxase/conjugal transfer nickase-helicase trwC, at 488,768 to 491,578
_00474
Position (kb) 487
488
489 Strain fitness (log2 ratio) -2
-1
0
1
2 at 486.099 kb on - strand at 486.177 kb on - strand at 486.177 kb on - strand at 486.242 kb on + strand at 486.242 kb on + strand at 486.250 kb on - strand at 486.255 kb on + strand at 486.255 kb on + strand at 486.315 kb on + strand, within CCNA_00471 at 486.323 kb on - strand, within CCNA_00471 at 486.323 kb on - strand, within CCNA_00471 at 486.334 kb on + strand, within CCNA_00471 at 486.351 kb on - strand, within CCNA_00471 at 486.353 kb on - strand, within CCNA_00471 at 486.376 kb on + strand, within CCNA_00471 at 486.386 kb on - strand, within CCNA_00471 at 486.475 kb on + strand, within CCNA_00471 at 486.492 kb on - strand, within CCNA_00471 at 486.509 kb on - strand, within CCNA_00471 at 486.528 kb on + strand, within CCNA_00471 at 486.531 kb on - strand, within CCNA_00471 at 486.536 kb on - strand, within CCNA_00471 at 486.536 kb on - strand, within CCNA_00471 at 486.541 kb on - strand, within CCNA_00471 at 486.577 kb on + strand, within CCNA_00471 at 486.599 kb on - strand, within CCNA_00471 at 486.609 kb on - strand, within CCNA_00471 at 486.626 kb on - strand, within CCNA_00471 at 486.634 kb on - strand, within CCNA_00471 at 486.636 kb on + strand, within CCNA_00471 at 486.636 kb on + strand, within CCNA_00471 at 486.637 kb on + strand, within CCNA_00471 at 486.637 kb on + strand, within CCNA_00471 at 486.637 kb on + strand, within CCNA_00471 at 486.644 kb on - strand, within CCNA_00471 at 486.644 kb on - strand, within CCNA_00471 at 486.645 kb on - strand, within CCNA_00471 at 486.645 kb on - strand, within CCNA_00471 at 486.645 kb on - strand, within CCNA_00471 at 486.645 kb on - strand, within CCNA_00471 at 486.647 kb on + strand, within CCNA_00471 at 486.655 kb on - strand, within CCNA_00471 at 486.693 kb on + strand, within CCNA_00471 at 486.700 kb on - strand, within CCNA_00471 at 486.700 kb on - strand, within CCNA_00471 at 486.711 kb on - strand, within CCNA_00471 at 486.716 kb on - strand, within CCNA_00471 at 486.751 kb on - strand, within CCNA_00471 at 486.762 kb on - strand, within CCNA_00471 at 486.771 kb on + strand, within CCNA_00471 at 486.774 kb on - strand, within CCNA_00471 at 486.779 kb on - strand, within CCNA_00471 at 486.790 kb on + strand, within CCNA_00471 at 486.790 kb on + strand, within CCNA_00471 at 486.798 kb on - strand, within CCNA_00471 at 486.826 kb on + strand, within CCNA_00471 at 486.845 kb on - strand, within CCNA_00471 at 486.888 kb on - strand, within CCNA_00471 at 486.888 kb on - strand, within CCNA_00471 at 486.895 kb on - strand, within CCNA_00471 at 486.898 kb on + strand, within CCNA_00471 at 486.969 kb on - strand, within CCNA_00471 at 486.971 kb on - strand, within CCNA_00471 at 487.028 kb on - strand, within CCNA_00471 at 487.038 kb on - strand at 487.058 kb on + strand at 487.065 kb on - strand at 487.101 kb on - strand at 487.132 kb on - strand at 487.154 kb on + strand at 487.154 kb on + strand at 487.154 kb on + strand at 487.154 kb on + strand at 487.157 kb on - strand at 487.157 kb on - strand at 487.157 kb on - strand at 487.159 kb on + strand at 487.162 kb on - strand at 487.164 kb on + strand at 487.172 kb on - strand at 487.207 kb on - strand, within CCNA_00472 at 487.207 kb on - strand, within CCNA_00472 at 487.207 kb on - strand, within CCNA_00472 at 487.207 kb on - strand, within CCNA_00472 at 487.217 kb on + strand, within CCNA_00472 at 487.245 kb on + strand, within CCNA_00472 at 487.245 kb on + strand, within CCNA_00472 at 487.253 kb on - strand, within CCNA_00472 at 487.266 kb on - strand, within CCNA_00472 at 487.273 kb on - strand, within CCNA_00472 at 487.309 kb on - strand, within CCNA_00472 at 487.323 kb on + strand, within CCNA_00472 at 487.407 kb on - strand, within CCNA_00472 at 487.407 kb on - strand, within CCNA_00472 at 487.442 kb on - strand, within CCNA_00472 at 487.495 kb on - strand, within CCNA_00472 at 487.495 kb on - strand, within CCNA_00472 at 487.503 kb on + strand, within CCNA_00472 at 487.510 kb on - strand, within CCNA_00472 at 487.513 kb on + strand, within CCNA_00472 at 487.516 kb on - strand, within CCNA_00472 at 487.523 kb on - strand, within CCNA_00472 at 487.528 kb on + strand, within CCNA_00472 at 487.561 kb on - strand, within CCNA_00472 at 487.603 kb on - strand, within CCNA_00472 at 487.635 kb on - strand, within CCNA_00472 at 487.653 kb on + strand, within CCNA_00472 at 487.663 kb on - strand, within CCNA_00472 at 487.664 kb on + strand, within CCNA_00472 at 487.664 kb on + strand, within CCNA_00472 at 487.664 kb on + strand, within CCNA_00472 at 487.664 kb on + strand, within CCNA_00472 at 487.664 kb on + strand, within CCNA_00472 at 487.664 kb on + strand, within CCNA_00472 at 487.664 kb on + strand, within CCNA_00472 at 487.672 kb on - strand, within CCNA_00472 at 487.672 kb on - strand, within CCNA_00472 at 487.672 kb on - strand, within CCNA_00472 at 487.672 kb on - strand, within CCNA_00472 at 487.682 kb on + strand, within CCNA_00472 at 487.683 kb on + strand, within CCNA_00472 at 487.715 kb on - strand, within CCNA_00472 at 487.728 kb on + strand, within CCNA_00472 at 487.729 kb on + strand, within CCNA_00472 at 487.739 kb on + strand, within CCNA_00472 at 487.739 kb on + strand, within CCNA_00472 at 487.739 kb on + strand, within CCNA_00472 at 487.747 kb on - strand, within CCNA_00472 at 487.749 kb on + strand, within CCNA_00472 at 487.749 kb on + strand, within CCNA_00472 at 487.752 kb on - strand, within CCNA_00472 at 487.765 kb on - strand, within CCNA_00472 at 487.805 kb on - strand, within CCNA_00472 at 487.808 kb on + strand, within CCNA_00472 at 487.829 kb on + strand, within CCNA_00472 at 487.837 kb on - strand, within CCNA_00472 at 487.871 kb on - strand, within CCNA_00472 at 487.875 kb on + strand, within CCNA_00472 at 487.881 kb on - strand, within CCNA_00472 at 487.893 kb on - strand, within CCNA_00472 at 487.907 kb on - strand, within CCNA_00472 at 487.975 kb on - strand, within CCNA_00472 at 487.989 kb on + strand, within CCNA_00472 at 487.997 kb on - strand, within CCNA_00472 at 488.031 kb on - strand, within CCNA_00472 at 488.033 kb on + strand, within CCNA_00472 at 488.041 kb on - strand, within CCNA_00472 at 488.041 kb on - strand, within CCNA_00472 at 488.055 kb on + strand, within CCNA_00472 at 488.055 kb on + strand, within CCNA_00472 at 488.063 kb on - strand at 488.063 kb on - strand at 488.063 kb on - strand at 488.063 kb on - strand at 488.070 kb on + strand at 488.070 kb on + strand at 488.070 kb on + strand at 488.070 kb on + strand at 488.070 kb on + strand at 488.072 kb on + strand at 488.072 kb on + strand at 488.072 kb on + strand at 488.072 kb on + strand at 488.075 kb on - strand at 488.078 kb on + strand at 488.078 kb on - strand at 488.078 kb on - strand at 488.078 kb on - strand at 488.080 kb on - strand at 488.080 kb on - strand at 488.080 kb on - strand at 488.102 kb on - strand at 488.102 kb on - strand at 488.129 kb on + strand at 488.129 kb on + strand at 488.129 kb on + strand at 488.135 kb on - strand at 488.137 kb on - strand at 488.139 kb on + strand at 488.139 kb on + strand at 488.179 kb on + strand at 488.181 kb on - strand at 488.218 kb on + strand at 488.226 kb on - strand at 488.228 kb on + strand at 488.243 kb on - strand at 488.281 kb on - strand at 488.299 kb on + strand at 488.308 kb on - strand at 488.318 kb on - strand at 488.401 kb on + strand, within CCNA_00473 at 488.401 kb on + strand, within CCNA_00473 at 488.402 kb on + strand, within CCNA_00473 at 488.409 kb on - strand, within CCNA_00473 at 488.409 kb on - strand, within CCNA_00473 at 488.409 kb on - strand, within CCNA_00473 at 488.504 kb on + strand, within CCNA_00473 at 488.512 kb on + strand, within CCNA_00473 at 488.535 kb on - strand, within CCNA_00473 at 488.609 kb on + strand, within CCNA_00473 at 488.609 kb on - strand, within CCNA_00473 at 488.609 kb on - strand, within CCNA_00473 at 488.635 kb on - strand, within CCNA_00473 at 488.682 kb on + strand, within CCNA_00473 at 488.688 kb on + strand, within CCNA_00473 at 488.722 kb on - strand at 488.768 kb on + strand at 488.768 kb on + strand at 488.768 kb on + strand at 488.768 kb on + strand at 488.776 kb on - strand at 488.776 kb on - strand at 488.776 kb on - strand at 488.793 kb on - strand at 488.825 kb on - strand at 488.826 kb on + strand at 488.834 kb on + strand at 488.851 kb on + strand at 488.851 kb on + strand at 488.859 kb on - strand at 488.859 kb on - strand at 488.867 kb on - strand at 488.869 kb on + strand at 488.869 kb on + strand at 488.879 kb on + strand at 488.887 kb on - strand at 488.931 kb on + strand at 488.950 kb on + strand at 488.950 kb on + strand at 488.958 kb on - strand at 488.962 kb on + strand at 488.970 kb on - strand at 489.007 kb on - strand at 489.007 kb on - strand at 489.007 kb on - strand at 489.026 kb on - strand at 489.046 kb on - strand at 489.058 kb on + strand, within CCNA_00474 at 489.058 kb on + strand, within CCNA_00474 at 489.062 kb on + strand, within CCNA_00474 at 489.064 kb on + strand, within CCNA_00474 at 489.064 kb on + strand, within CCNA_00474 at 489.064 kb on + strand, within CCNA_00474 at 489.070 kb on - strand, within CCNA_00474 at 489.072 kb on - strand, within CCNA_00474 at 489.072 kb on - strand, within CCNA_00474 at 489.077 kb on + strand, within CCNA_00474 at 489.098 kb on + strand, within CCNA_00474 at 489.098 kb on + strand, within CCNA_00474 at 489.098 kb on + strand, within CCNA_00474 at 489.100 kb on + strand, within CCNA_00474 at 489.106 kb on - strand, within CCNA_00474 at 489.120 kb on + strand, within CCNA_00474 at 489.169 kb on - strand, within CCNA_00474 at 489.169 kb on - strand, within CCNA_00474
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with CHIR-090 10 ug/mL remove 486,099 - +1.6 486,177 - -1.2 486,177 - +0.3 486,242 + -0.1 486,242 + +0.1 486,250 - +0.1 486,255 + +0.1 486,255 + +1.7 486,315 + CCNA_00471 0.16 -0.9 486,323 - CCNA_00471 0.17 +0.2 486,323 - CCNA_00471 0.17 +0.5 486,334 + CCNA_00471 0.18 -0.6 486,351 - CCNA_00471 0.20 +1.8 486,353 - CCNA_00471 0.20 -2.3 486,376 + CCNA_00471 0.22 -0.1 486,386 - CCNA_00471 0.23 -0.6 486,475 + CCNA_00471 0.33 +0.8 486,492 - CCNA_00471 0.34 +0.8 486,509 - CCNA_00471 0.36 +0.7 486,528 + CCNA_00471 0.38 +0.7 486,531 - CCNA_00471 0.38 -0.5 486,536 - CCNA_00471 0.39 +0.4 486,536 - CCNA_00471 0.39 +0.0 486,541 - CCNA_00471 0.39 +0.6 486,577 + CCNA_00471 0.43 -2.6 486,599 - CCNA_00471 0.45 +0.5 486,609 - CCNA_00471 0.46 +1.0 486,626 - CCNA_00471 0.48 +0.9 486,634 - CCNA_00471 0.49 +0.3 486,636 + CCNA_00471 0.49 +0.0 486,636 + CCNA_00471 0.49 -0.3 486,637 + CCNA_00471 0.49 +0.5 486,637 + CCNA_00471 0.49 +0.8 486,637 + CCNA_00471 0.49 -0.0 486,644 - CCNA_00471 0.50 -0.3 486,644 - CCNA_00471 0.50 +0.5 486,645 - CCNA_00471 0.50 -1.1 486,645 - CCNA_00471 0.50 +1.2 486,645 - CCNA_00471 0.50 +0.3 486,645 - CCNA_00471 0.50 -0.2 486,647 + CCNA_00471 0.50 +0.4 486,655 - CCNA_00471 0.51 +0.5 486,693 + CCNA_00471 0.55 +0.1 486,700 - CCNA_00471 0.56 -2.5 486,700 - CCNA_00471 0.56 +0.6 486,711 - CCNA_00471 0.57 -0.1 486,716 - CCNA_00471 0.57 +0.1 486,751 - CCNA_00471 0.61 +0.2 486,762 - CCNA_00471 0.62 -0.6 486,771 + CCNA_00471 0.63 +1.5 486,774 - CCNA_00471 0.63 +0.7 486,779 - CCNA_00471 0.64 +0.6 486,790 + CCNA_00471 0.65 -1.3 486,790 + CCNA_00471 0.65 +1.1 486,798 - CCNA_00471 0.66 +0.5 486,826 + CCNA_00471 0.69 +0.4 486,845 - CCNA_00471 0.71 +0.2 486,888 - CCNA_00471 0.75 +0.1 486,888 - CCNA_00471 0.75 +0.5 486,895 - CCNA_00471 0.76 +2.2 486,898 + CCNA_00471 0.76 +0.1 486,969 - CCNA_00471 0.84 -0.7 486,971 - CCNA_00471 0.84 +1.4 487,028 - CCNA_00471 0.90 -1.4 487,038 - -0.1 487,058 + -0.9 487,065 - +0.6 487,101 - +0.4 487,132 - +0.3 487,154 + -0.3 487,154 + -0.0 487,154 + -0.7 487,154 + +0.8 487,157 - -0.4 487,157 - +0.8 487,157 - -0.1 487,159 + +0.0 487,162 - +1.0 487,164 + +0.3 487,172 - -0.4 487,207 - CCNA_00472 0.11 -0.5 487,207 - CCNA_00472 0.11 +0.7 487,207 - CCNA_00472 0.11 +1.6 487,207 - CCNA_00472 0.11 +1.2 487,217 + CCNA_00472 0.12 +0.3 487,245 + CCNA_00472 0.14 -1.2 487,245 + CCNA_00472 0.14 +1.6 487,253 - CCNA_00472 0.15 +0.4 487,266 - CCNA_00472 0.16 +0.6 487,273 - CCNA_00472 0.17 +0.8 487,309 - CCNA_00472 0.20 +0.4 487,323 + CCNA_00472 0.21 -0.4 487,407 - CCNA_00472 0.29 -1.0 487,407 - CCNA_00472 0.29 +0.7 487,442 - CCNA_00472 0.32 +0.4 487,495 - CCNA_00472 0.37 +0.5 487,495 - CCNA_00472 0.37 +1.0 487,503 + CCNA_00472 0.38 +0.0 487,510 - CCNA_00472 0.39 -0.2 487,513 + CCNA_00472 0.39 +0.0 487,516 - CCNA_00472 0.39 -0.5 487,523 - CCNA_00472 0.40 +1.7 487,528 + CCNA_00472 0.40 -0.3 487,561 - CCNA_00472 0.43 -0.3 487,603 - CCNA_00472 0.47 +0.0 487,635 - CCNA_00472 0.50 +0.7 487,653 + CCNA_00472 0.52 +0.2 487,663 - CCNA_00472 0.53 +0.5 487,664 + CCNA_00472 0.53 +0.1 487,664 + CCNA_00472 0.53 -0.1 487,664 + CCNA_00472 0.53 -0.8 487,664 + CCNA_00472 0.53 -1.1 487,664 + CCNA_00472 0.53 +1.1 487,664 + CCNA_00472 0.53 +0.0 487,664 + CCNA_00472 0.53 -1.1 487,672 - CCNA_00472 0.54 +1.4 487,672 - CCNA_00472 0.54 +0.9 487,672 - CCNA_00472 0.54 +0.0 487,672 - CCNA_00472 0.54 -0.9 487,682 + CCNA_00472 0.55 +1.0 487,683 + CCNA_00472 0.55 +0.0 487,715 - CCNA_00472 0.58 +0.9 487,728 + CCNA_00472 0.59 +0.2 487,729 + CCNA_00472 0.59 +0.5 487,739 + CCNA_00472 0.60 -0.4 487,739 + CCNA_00472 0.60 -0.8 487,739 + CCNA_00472 0.60 +1.5 487,747 - CCNA_00472 0.61 -0.2 487,749 + CCNA_00472 0.61 -0.0 487,749 + CCNA_00472 0.61 -0.9 487,752 - CCNA_00472 0.61 +0.6 487,765 - CCNA_00472 0.62 +1.2 487,805 - CCNA_00472 0.66 +0.3 487,808 + CCNA_00472 0.66 -1.4 487,829 + CCNA_00472 0.68 +0.4 487,837 - CCNA_00472 0.69 -0.7 487,871 - CCNA_00472 0.72 +0.9 487,875 + CCNA_00472 0.73 -1.6 487,881 - CCNA_00472 0.73 +0.5 487,893 - CCNA_00472 0.74 +1.4 487,907 - CCNA_00472 0.76 +1.1 487,975 - CCNA_00472 0.82 +0.7 487,989 + CCNA_00472 0.83 -1.6 487,997 - CCNA_00472 0.84 +0.3 488,031 - CCNA_00472 0.87 +0.5 488,033 + CCNA_00472 0.87 +0.8 488,041 - CCNA_00472 0.88 +0.6 488,041 - CCNA_00472 0.88 +0.0 488,055 + CCNA_00472 0.89 -0.9 488,055 + CCNA_00472 0.89 +1.1 488,063 - +0.7 488,063 - +0.1 488,063 - +0.3 488,063 - +0.4 488,070 + -1.6 488,070 + -0.3 488,070 + -0.1 488,070 + -1.8 488,070 + -1.1 488,072 + -0.8 488,072 + +0.0 488,072 + +0.2 488,072 + -0.6 488,075 - -0.3 488,078 + -0.6 488,078 - +0.5 488,078 - -0.4 488,078 - +0.8 488,080 - -0.7 488,080 - -0.3 488,080 - +0.1 488,102 - -0.1 488,102 - -0.4 488,129 + +0.4 488,129 + +0.8 488,129 + -1.8 488,135 - +0.4 488,137 - +1.2 488,139 + -1.0 488,139 + -0.0 488,179 + +0.0 488,181 - +0.7 488,218 + -0.1 488,226 - -0.7 488,228 + +0.1 488,243 - +0.1 488,281 - -0.5 488,299 + -0.3 488,308 - +1.2 488,318 - -1.4 488,401 + CCNA_00473 0.26 +1.2 488,401 + CCNA_00473 0.26 +1.1 488,402 + CCNA_00473 0.26 -0.9 488,409 - CCNA_00473 0.27 +0.2 488,409 - CCNA_00473 0.27 -0.3 488,409 - CCNA_00473 0.27 +0.2 488,504 + CCNA_00473 0.46 +0.2 488,512 + CCNA_00473 0.48 +0.4 488,535 - CCNA_00473 0.53 -0.2 488,609 + CCNA_00473 0.68 -0.1 488,609 - CCNA_00473 0.68 -1.3 488,609 - CCNA_00473 0.68 -0.6 488,635 - CCNA_00473 0.73 -0.1 488,682 + CCNA_00473 0.82 +0.2 488,688 + CCNA_00473 0.84 -1.3 488,722 - -0.8 488,768 + +0.4 488,768 + -0.5 488,768 + +0.0 488,768 + +0.0 488,776 - -1.3 488,776 - -0.1 488,776 - +0.2 488,793 - +0.2 488,825 - +0.5 488,826 + +0.6 488,834 + +0.8 488,851 + -0.0 488,851 + -0.0 488,859 - +0.3 488,859 - +0.1 488,867 - +0.1 488,869 + -1.1 488,869 + +0.1 488,879 + -0.6 488,887 - +1.0 488,931 + -0.5 488,950 + +1.0 488,950 + -0.1 488,958 - +0.4 488,962 + -0.2 488,970 - -1.1 489,007 - -1.5 489,007 - -0.4 489,007 - -1.3 489,026 - -0.2 489,046 - -1.5 489,058 + CCNA_00474 0.10 -0.6 489,058 + CCNA_00474 0.10 +2.3 489,062 + CCNA_00474 0.10 -0.9 489,064 + CCNA_00474 0.11 -0.6 489,064 + CCNA_00474 0.11 +0.0 489,064 + CCNA_00474 0.11 +1.1 489,070 - CCNA_00474 0.11 -0.0 489,072 - CCNA_00474 0.11 +0.4 489,072 - CCNA_00474 0.11 +0.3 489,077 + CCNA_00474 0.11 -0.8 489,098 + CCNA_00474 0.12 -0.9 489,098 + CCNA_00474 0.12 +0.3 489,098 + CCNA_00474 0.12 +0.4 489,100 + CCNA_00474 0.12 -0.2 489,106 - CCNA_00474 0.12 -1.2 489,120 + CCNA_00474 0.13 +0.3 489,169 - CCNA_00474 0.14 -0.3 489,169 - CCNA_00474 0.14 -1.6
Or see this region's nucleotide sequence