Experiment: PYE with CHIR-090 10 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_00104 and CCNA_00105 are separated by 97 nucleotides CCNA_00105 and CCNA_00106 are separated by 47 nucleotides CCNA_00106 and CCNA_00107 are separated by 160 nucleotides CCNA_00107 and CCNA_00108 are separated by 9 nucleotides
CCNA_00104: CCNA_00104 - orotidine 5'-phosphate decarboxylase, at 115,289 to 115,990
_00104
CCNA_00105: CCNA_00105 - MarC family integral membrane protein, at 116,088 to 116,786
_00105
CCNA_00106: CCNA_00106 - 2-polyprenyl-6-methoxyphenol hydroxylase, at 116,834 to 118,099
_00106
CCNA_00107: CCNA_00107 - Trm112p-like protein, at 118,260 to 118,457
_00107
CCNA_00108: CCNA_00108 - lon endopeptidase-related protein, at 118,467 to 119,144
_00108
Position (kb) 116
117
118
119 Strain fitness (log2 ratio) -2
-1
0
1 at 115.835 kb on - strand, within CCNA_00104 at 115.847 kb on + strand, within CCNA_00104 at 115.852 kb on + strand, within CCNA_00104 at 115.860 kb on - strand, within CCNA_00104 at 115.876 kb on + strand, within CCNA_00104 at 115.876 kb on + strand, within CCNA_00104 at 115.876 kb on + strand, within CCNA_00104 at 115.947 kb on - strand at 116.003 kb on - strand at 116.056 kb on + strand at 116.067 kb on + strand at 116.783 kb on + strand at 116.784 kb on - strand at 116.794 kb on + strand at 116.794 kb on + strand at 116.794 kb on + strand at 116.802 kb on - strand at 116.826 kb on - strand at 116.875 kb on - strand at 118.128 kb on - strand at 118.156 kb on + strand at 118.162 kb on + strand at 118.172 kb on + strand at 118.179 kb on + strand at 118.259 kb on - strand at 118.461 kb on - strand at 118.461 kb on - strand at 118.461 kb on - strand at 118.465 kb on + strand at 118.513 kb on + strand at 118.521 kb on - strand at 118.531 kb on - strand at 118.532 kb on + strand at 118.532 kb on + strand at 118.532 kb on + strand at 118.537 kb on + strand, within CCNA_00108 at 118.540 kb on - strand, within CCNA_00108 at 118.561 kb on + strand, within CCNA_00108 at 118.574 kb on - strand, within CCNA_00108 at 118.604 kb on - strand, within CCNA_00108 at 118.611 kb on - strand, within CCNA_00108 at 118.611 kb on - strand, within CCNA_00108 at 118.617 kb on + strand, within CCNA_00108 at 118.623 kb on - strand, within CCNA_00108 at 118.629 kb on + strand, within CCNA_00108 at 118.684 kb on + strand, within CCNA_00108 at 118.684 kb on + strand, within CCNA_00108 at 118.684 kb on + strand, within CCNA_00108 at 118.770 kb on - strand, within CCNA_00108 at 118.828 kb on + strand, within CCNA_00108 at 118.878 kb on + strand, within CCNA_00108 at 118.891 kb on - strand, within CCNA_00108 at 118.914 kb on - strand, within CCNA_00108 at 118.925 kb on + strand, within CCNA_00108 at 118.968 kb on + strand, within CCNA_00108 at 118.971 kb on - strand, within CCNA_00108 at 118.976 kb on - strand, within CCNA_00108 at 119.033 kb on + strand, within CCNA_00108 at 119.033 kb on + strand, within CCNA_00108 at 119.041 kb on - strand, within CCNA_00108
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with CHIR-090 10 ug/mL remove 115,835 - CCNA_00104 0.78 -0.7 115,847 + CCNA_00104 0.79 -0.5 115,852 + CCNA_00104 0.80 -1.2 115,860 - CCNA_00104 0.81 +0.3 115,876 + CCNA_00104 0.84 -0.9 115,876 + CCNA_00104 0.84 +0.9 115,876 + CCNA_00104 0.84 -1.3 115,947 - -0.9 116,003 - -0.2 116,056 + +0.3 116,067 + -0.2 116,783 + +0.0 116,784 - -0.1 116,794 + -1.2 116,794 + +0.3 116,794 + -0.0 116,802 - +0.9 116,826 - -0.4 116,875 - +0.1 118,128 - -0.4 118,156 + +1.1 118,162 + -1.8 118,172 + -1.7 118,179 + -0.3 118,259 - -0.0 118,461 - -0.5 118,461 - +0.5 118,461 - -0.2 118,465 + -0.9 118,513 + +0.2 118,521 - +0.9 118,531 - -1.5 118,532 + -0.6 118,532 + -1.4 118,532 + -0.7 118,537 + CCNA_00108 0.10 +0.0 118,540 - CCNA_00108 0.11 -0.1 118,561 + CCNA_00108 0.14 -0.5 118,574 - CCNA_00108 0.16 +0.1 118,604 - CCNA_00108 0.20 +0.1 118,611 - CCNA_00108 0.21 -0.3 118,611 - CCNA_00108 0.21 -0.2 118,617 + CCNA_00108 0.22 -0.3 118,623 - CCNA_00108 0.23 +0.9 118,629 + CCNA_00108 0.24 +1.4 118,684 + CCNA_00108 0.32 +0.1 118,684 + CCNA_00108 0.32 +0.1 118,684 + CCNA_00108 0.32 +0.0 118,770 - CCNA_00108 0.45 -0.2 118,828 + CCNA_00108 0.53 +0.2 118,878 + CCNA_00108 0.61 -1.1 118,891 - CCNA_00108 0.63 +0.6 118,914 - CCNA_00108 0.66 -0.1 118,925 + CCNA_00108 0.68 -0.6 118,968 + CCNA_00108 0.74 +0.9 118,971 - CCNA_00108 0.74 +0.1 118,976 - CCNA_00108 0.75 +0.6 119,033 + CCNA_00108 0.83 -1.1 119,033 + CCNA_00108 0.83 +0.8 119,041 - CCNA_00108 0.85 +0.1
Or see this region's nucleotide sequence