Experiment: PYE with CHIR-090 10 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_00079 and CCNA_00080 are separated by 193 nucleotides CCNA_00080 and CCNA_00081 are separated by 34 nucleotides CCNA_00081 and CCNA_00082 are separated by 249 nucleotides
CCNA_00079: CCNA_00079 - MerR-family transcriptional regulator, at 83,414 to 83,893
_00079
CCNA_00080: CCNA_00080 - LexA-related transcriptional repressor, at 84,087 to 84,728
_00080
CCNA_00081: CCNA_00081 - hypothetical protein, at 84,763 to 84,972
_00081
CCNA_00082: CCNA_00082 - class 1 lysyl-tRNA synthetase, at 85,222 to 86,880
_00082
Position (kb) 84
85 Strain fitness (log2 ratio) -2
-1
0
1
2 at 83.768 kb on - strand, within CCNA_00079 at 83.781 kb on + strand, within CCNA_00079 at 83.783 kb on + strand, within CCNA_00079 at 83.838 kb on + strand, within CCNA_00079 at 83.968 kb on - strand at 84.196 kb on + strand, within CCNA_00080 at 84.196 kb on + strand, within CCNA_00080 at 84.453 kb on - strand, within CCNA_00080 at 84.458 kb on - strand, within CCNA_00080 at 84.541 kb on + strand, within CCNA_00080 at 84.551 kb on + strand, within CCNA_00080 at 84.559 kb on - strand, within CCNA_00080 at 84.594 kb on + strand, within CCNA_00080 at 84.602 kb on - strand, within CCNA_00080 at 84.653 kb on - strand, within CCNA_00080 at 84.751 kb on + strand at 84.753 kb on - strand at 84.758 kb on - strand at 84.758 kb on - strand at 84.766 kb on + strand at 84.769 kb on - strand at 84.782 kb on - strand at 84.795 kb on + strand, within CCNA_00081 at 84.830 kb on + strand, within CCNA_00081 at 84.835 kb on + strand, within CCNA_00081 at 84.838 kb on - strand, within CCNA_00081 at 84.838 kb on - strand, within CCNA_00081 at 84.876 kb on - strand, within CCNA_00081 at 84.886 kb on - strand, within CCNA_00081 at 84.886 kb on - strand, within CCNA_00081 at 84.886 kb on - strand, within CCNA_00081 at 84.886 kb on - strand, within CCNA_00081 at 84.909 kb on - strand, within CCNA_00081 at 84.911 kb on - strand, within CCNA_00081 at 84.928 kb on + strand, within CCNA_00081 at 84.957 kb on + strand at 84.957 kb on + strand at 84.957 kb on + strand at 84.965 kb on - strand at 84.965 kb on - strand at 84.965 kb on - strand at 84.969 kb on - strand at 84.969 kb on - strand at 84.974 kb on + strand at 84.977 kb on - strand at 84.982 kb on - strand at 84.982 kb on - strand at 84.991 kb on - strand at 85.009 kb on + strand at 85.014 kb on + strand at 85.022 kb on - strand at 85.038 kb on - strand at 85.040 kb on + strand at 85.040 kb on + strand at 85.062 kb on + strand at 85.081 kb on + strand at 85.085 kb on + strand at 85.091 kb on - strand at 85.125 kb on + strand at 85.125 kb on + strand at 85.133 kb on - strand at 85.158 kb on - strand at 85.161 kb on - strand at 85.174 kb on + strand at 85.206 kb on + strand at 85.214 kb on - strand at 85.252 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with CHIR-090 10 ug/mL remove 83,768 - CCNA_00079 0.74 +1.1 83,781 + CCNA_00079 0.76 +0.8 83,783 + CCNA_00079 0.77 -0.9 83,838 + CCNA_00079 0.88 -2.2 83,968 - -1.0 84,196 + CCNA_00080 0.17 +2.2 84,196 + CCNA_00080 0.17 -0.5 84,453 - CCNA_00080 0.57 +0.5 84,458 - CCNA_00080 0.58 -0.6 84,541 + CCNA_00080 0.71 +0.9 84,551 + CCNA_00080 0.72 +2.0 84,559 - CCNA_00080 0.74 -0.3 84,594 + CCNA_00080 0.79 +1.3 84,602 - CCNA_00080 0.80 +0.6 84,653 - CCNA_00080 0.88 +1.6 84,751 + -1.1 84,753 - +0.1 84,758 - +1.1 84,758 - -0.4 84,766 + +0.9 84,769 - -0.9 84,782 - +0.7 84,795 + CCNA_00081 0.15 +0.4 84,830 + CCNA_00081 0.32 -0.2 84,835 + CCNA_00081 0.34 -0.5 84,838 - CCNA_00081 0.36 -0.1 84,838 - CCNA_00081 0.36 +0.7 84,876 - CCNA_00081 0.54 -0.2 84,886 - CCNA_00081 0.59 +0.1 84,886 - CCNA_00081 0.59 -1.0 84,886 - CCNA_00081 0.59 +0.4 84,886 - CCNA_00081 0.59 -0.1 84,909 - CCNA_00081 0.70 +0.1 84,911 - CCNA_00081 0.70 -0.7 84,928 + CCNA_00081 0.79 -0.1 84,957 + -1.2 84,957 + -0.2 84,957 + +0.8 84,965 - +0.4 84,965 - +0.3 84,965 - -0.2 84,969 - -0.1 84,969 - +1.0 84,974 + +0.1 84,977 - +0.5 84,982 - +0.1 84,982 - +0.5 84,991 - +0.0 85,009 + -0.4 85,014 + -1.7 85,022 - +0.8 85,038 - -0.3 85,040 + -0.5 85,040 + -0.3 85,062 + +0.5 85,081 + -1.5 85,085 + -0.3 85,091 - +0.0 85,125 + +0.3 85,125 + +0.7 85,133 - +0.5 85,158 - -0.1 85,161 - +1.0 85,174 + -0.6 85,206 + -0.7 85,214 - +0.8 85,252 + +0.2
Or see this region's nucleotide sequence