Experiment: PYE with CHIR-090 10 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_00043 and CCNA_00044 are separated by 2 nucleotides CCNA_00044 and CCNA_00045 are separated by 258 nucleotides CCNA_00045 and CCNA_00046 are separated by 6 nucleotides
CCNA_00043: CCNA_00043 - N utilization substance protein A NusA, at 46,622 to 48,268
_00043
CCNA_00044: CCNA_00044 - ribosome maturation protein RimP, at 48,271 to 48,846
_00044
CCNA_00045: CCNA_00045 - inorganic pyrophosphatase, at 49,105 to 49,638
_00045
CCNA_00046: CCNA_00046 - hypothetical protein, at 49,645 to 50,031
_00046
Position (kb) 48
49 Strain fitness (log2 ratio) -1
0
1
2 at 48.302 kb on - strand at 48.302 kb on - strand at 48.302 kb on - strand at 48.349 kb on - strand, within CCNA_00044 at 48.349 kb on - strand, within CCNA_00044 at 48.359 kb on + strand, within CCNA_00044 at 48.367 kb on - strand, within CCNA_00044 at 48.438 kb on - strand, within CCNA_00044 at 48.860 kb on - strand at 49.643 kb on + strand at 49.648 kb on + strand at 49.648 kb on + strand at 49.648 kb on + strand at 49.648 kb on + strand at 49.661 kb on + strand at 49.661 kb on + strand at 49.661 kb on + strand at 49.673 kb on + strand at 49.681 kb on - strand at 49.681 kb on - strand at 49.710 kb on - strand, within CCNA_00046 at 49.736 kb on - strand, within CCNA_00046 at 49.752 kb on - strand, within CCNA_00046 at 49.837 kb on + strand, within CCNA_00046 at 49.837 kb on + strand, within CCNA_00046 at 49.845 kb on - strand, within CCNA_00046
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with CHIR-090 10 ug/mL remove 48,302 - -1.1 48,302 - -0.7 48,302 - +2.0 48,349 - CCNA_00044 0.14 -0.4 48,349 - CCNA_00044 0.14 -1.6 48,359 + CCNA_00044 0.15 -0.2 48,367 - CCNA_00044 0.17 -0.7 48,438 - CCNA_00044 0.29 +0.8 48,860 - -1.1 49,643 + +0.2 49,648 + -0.4 49,648 + +0.4 49,648 + +0.8 49,648 + +0.1 49,661 + -0.4 49,661 + -0.1 49,661 + +0.6 49,673 + +0.1 49,681 - -0.4 49,681 - +0.5 49,710 - CCNA_00046 0.17 -1.2 49,736 - CCNA_00046 0.24 +0.4 49,752 - CCNA_00046 0.28 +0.2 49,837 + CCNA_00046 0.50 -0.8 49,837 + CCNA_00046 0.50 +0.1 49,845 - CCNA_00046 0.52 -0.4
Or see this region's nucleotide sequence