Experiment: Neomycin 0.04 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_04475 and PS417_04480 are separated by 57 nucleotides PS417_04480 and PS417_04485 are separated by 182 nucleotides PS417_04485 and PS417_04490 are separated by 150 nucleotides
PS417_04475: PS417_04475 - hypothetical protein, at 991,354 to 991,941
_04475
PS417_04480: PS417_04480 - hypothetical protein, at 991,999 to 992,208
_04480
PS417_04485: PS417_04485 - hypothetical protein, at 992,391 to 992,648
_04485
PS417_04490: PS417_04490 - glycerol acyltransferase, at 992,799 to 993,962
_04490
Position (kb) 992
993 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 991.589 kb on + strand, within PS417_04475 at 991.589 kb on + strand, within PS417_04475 at 991.590 kb on - strand, within PS417_04475 at 991.702 kb on + strand, within PS417_04475 at 991.703 kb on - strand, within PS417_04475 at 991.726 kb on + strand, within PS417_04475 at 991.726 kb on + strand, within PS417_04475 at 991.726 kb on + strand, within PS417_04475 at 991.727 kb on - strand, within PS417_04475 at 992.096 kb on + strand, within PS417_04480 at 992.178 kb on - strand, within PS417_04480 at 992.219 kb on + strand at 992.249 kb on + strand at 992.278 kb on + strand at 992.286 kb on - strand at 992.286 kb on - strand at 992.291 kb on - strand at 992.316 kb on + strand at 992.335 kb on - strand at 992.377 kb on + strand at 992.378 kb on - strand at 992.429 kb on - strand, within PS417_04485 at 992.429 kb on - strand, within PS417_04485 at 992.431 kb on + strand, within PS417_04485 at 992.431 kb on + strand, within PS417_04485 at 992.432 kb on - strand, within PS417_04485 at 992.432 kb on - strand, within PS417_04485 at 992.432 kb on - strand, within PS417_04485 at 992.554 kb on + strand, within PS417_04485 at 992.555 kb on - strand, within PS417_04485 at 992.555 kb on - strand, within PS417_04485 at 992.726 kb on + strand at 992.727 kb on - strand at 992.800 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Neomycin 0.04 mg/ml remove 991,589 + PS417_04475 0.40 -2.3 991,589 + PS417_04475 0.40 -0.9 991,590 - PS417_04475 0.40 -1.3 991,702 + PS417_04475 0.59 -0.6 991,703 - PS417_04475 0.59 -0.5 991,726 + PS417_04475 0.63 +2.8 991,726 + PS417_04475 0.63 -0.3 991,726 + PS417_04475 0.63 -1.2 991,727 - PS417_04475 0.63 +2.2 992,096 + PS417_04480 0.46 -0.1 992,178 - PS417_04480 0.85 -0.2 992,219 + -0.9 992,249 + +0.3 992,278 + -0.6 992,286 - -1.1 992,286 - -1.6 992,291 - -0.3 992,316 + +0.4 992,335 - -2.4 992,377 + -1.8 992,378 - -1.2 992,429 - PS417_04485 0.15 -1.6 992,429 - PS417_04485 0.15 -1.9 992,431 + PS417_04485 0.16 -1.3 992,431 + PS417_04485 0.16 +0.2 992,432 - PS417_04485 0.16 -0.0 992,432 - PS417_04485 0.16 -1.0 992,432 - PS417_04485 0.16 -0.2 992,554 + PS417_04485 0.63 -0.5 992,555 - PS417_04485 0.64 -2.2 992,555 - PS417_04485 0.64 -1.3 992,726 + -0.0 992,727 - -1.1 992,800 + +0.4
Or see this region's nucleotide sequence