Strain Fitness in Pseudomonas simiae WCS417 around PS417_00435

Experiment: Neomycin 0.04 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPS417_00425 and PS417_00430 are separated by 10 nucleotidesPS417_00430 and PS417_00435 overlap by 4 nucleotidesPS417_00435 and PS417_00440 are separated by 88 nucleotidesPS417_00440 and PS417_00445 overlap by 4 nucleotides PS417_00425: PS417_00425 - thiol:disulfide interchange protein, at 82,991 to 83,626 _00425 PS417_00430: PS417_00430 - endonuclease, at 83,637 to 84,515 _00430 PS417_00435: PS417_00435 - DeoR faimly transcriptional regulator, at 84,512 to 86,566 _00435 PS417_00440: PS417_00440 - N-acetylmuramoyl-L-alanine amidase, at 86,655 to 87,431 _00440 PS417_00445: PS417_00445 - histidine kinase, at 87,428 to 89,215 _00445 Position (kb) 84 85 86 87Strain fitness (log2 ratio) -2 -1 0 1at 83.624 kb on + strandat 83.790 kb on + strand, within PS417_00430at 83.791 kb on - strand, within PS417_00430at 83.791 kb on - strand, within PS417_00430at 83.791 kb on - strand, within PS417_00430at 84.189 kb on + strand, within PS417_00430at 84.190 kb on - strand, within PS417_00430at 84.190 kb on - strand, within PS417_00430at 84.190 kb on - strand, within PS417_00430at 84.213 kb on + strand, within PS417_00430at 84.214 kb on - strand, within PS417_00430at 84.357 kb on - strand, within PS417_00430at 84.357 kb on - strand, within PS417_00430at 84.371 kb on + strand, within PS417_00430at 84.551 kb on - strandat 84.551 kb on - strandat 84.710 kb on - strandat 85.175 kb on - strand, within PS417_00435at 85.493 kb on + strand, within PS417_00435at 85.493 kb on + strand, within PS417_00435at 85.493 kb on + strand, within PS417_00435at 85.494 kb on - strand, within PS417_00435at 85.494 kb on - strand, within PS417_00435at 85.494 kb on - strand, within PS417_00435at 85.494 kb on - strand, within PS417_00435at 85.494 kb on - strand, within PS417_00435at 85.524 kb on - strand, within PS417_00435at 85.649 kb on + strand, within PS417_00435at 85.730 kb on + strand, within PS417_00435at 85.755 kb on + strand, within PS417_00435at 85.756 kb on - strand, within PS417_00435at 86.228 kb on + strand, within PS417_00435at 86.275 kb on - strand, within PS417_00435at 86.306 kb on - strand, within PS417_00435at 86.334 kb on + strand, within PS417_00435at 86.334 kb on + strand, within PS417_00435at 86.334 kb on + strand, within PS417_00435at 86.334 kb on + strand, within PS417_00435at 86.335 kb on - strand, within PS417_00435at 86.335 kb on - strand, within PS417_00435at 86.335 kb on - strand, within PS417_00435at 86.522 kb on + strandat 86.522 kb on + strandat 86.523 kb on - strandat 86.523 kb on - strandat 86.564 kb on + strandat 86.641 kb on + strandat 86.642 kb on - strandat 86.642 kb on - strandat 86.642 kb on - strandat 86.667 kb on + strandat 86.667 kb on + strandat 86.874 kb on + strand, within PS417_00440at 86.874 kb on + strand, within PS417_00440at 86.874 kb on + strand, within PS417_00440at 86.874 kb on + strand, within PS417_00440at 86.875 kb on - strand, within PS417_00440at 86.875 kb on - strand, within PS417_00440at 87.016 kb on - strand, within PS417_00440at 87.039 kb on - strand, within PS417_00440at 87.039 kb on - strand, within PS417_00440at 87.190 kb on + strand, within PS417_00440at 87.231 kb on + strand, within PS417_00440at 87.231 kb on + strand, within PS417_00440at 87.232 kb on - strand, within PS417_00440at 87.232 kb on - strand, within PS417_00440at 87.447 kb on + strandat 87.447 kb on + strandat 87.447 kb on + strandat 87.448 kb on - strandat 87.448 kb on - strandat 87.448 kb on - strandat 87.448 kb on - strandat 87.478 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Neomycin 0.04 mg/ml
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83,624 + -0.2
83,790 + PS417_00430 0.17 +0.6
83,791 - PS417_00430 0.18 +0.5
83,791 - PS417_00430 0.18 +0.4
83,791 - PS417_00430 0.18 +0.7
84,189 + PS417_00430 0.63 +0.8
84,190 - PS417_00430 0.63 +0.1
84,190 - PS417_00430 0.63 +0.2
84,190 - PS417_00430 0.63 +1.4
84,213 + PS417_00430 0.66 +0.5
84,214 - PS417_00430 0.66 +0.9
84,357 - PS417_00430 0.82 +0.8
84,357 - PS417_00430 0.82 +0.8
84,371 + PS417_00430 0.84 +0.4
84,551 - -0.7
84,551 - -0.5
84,710 - +0.1
85,175 - PS417_00435 0.32 -0.9
85,493 + PS417_00435 0.48 -0.0
85,493 + PS417_00435 0.48 -0.8
85,493 + PS417_00435 0.48 +0.2
85,494 - PS417_00435 0.48 -0.3
85,494 - PS417_00435 0.48 -1.0
85,494 - PS417_00435 0.48 +0.1
85,494 - PS417_00435 0.48 +0.7
85,494 - PS417_00435 0.48 +0.0
85,524 - PS417_00435 0.49 -0.1
85,649 + PS417_00435 0.55 +0.3
85,730 + PS417_00435 0.59 +0.4
85,755 + PS417_00435 0.60 -0.1
85,756 - PS417_00435 0.61 -0.6
86,228 + PS417_00435 0.84 +1.5
86,275 - PS417_00435 0.86 +0.9
86,306 - PS417_00435 0.87 -2.2
86,334 + PS417_00435 0.89 -0.9
86,334 + PS417_00435 0.89 -0.6
86,334 + PS417_00435 0.89 -0.4
86,334 + PS417_00435 0.89 -0.0
86,335 - PS417_00435 0.89 -0.2
86,335 - PS417_00435 0.89 +0.2
86,335 - PS417_00435 0.89 +0.2
86,522 + -0.5
86,522 + +0.1
86,523 - -0.9
86,523 - -0.9
86,564 + -0.1
86,641 + -1.0
86,642 - -1.4
86,642 - -1.0
86,642 - -1.1
86,667 + -0.9
86,667 + -1.5
86,874 + PS417_00440 0.28 -1.7
86,874 + PS417_00440 0.28 -2.0
86,874 + PS417_00440 0.28 -0.4
86,874 + PS417_00440 0.28 -1.1
86,875 - PS417_00440 0.28 -1.2
86,875 - PS417_00440 0.28 -1.8
87,016 - PS417_00440 0.46 -1.5
87,039 - PS417_00440 0.49 -1.7
87,039 - PS417_00440 0.49 -0.6
87,190 + PS417_00440 0.69 -1.2
87,231 + PS417_00440 0.74 -1.2
87,231 + PS417_00440 0.74 -1.0
87,232 - PS417_00440 0.74 -1.2
87,232 - PS417_00440 0.74 -1.9
87,447 + -0.6
87,447 + +0.4
87,447 + -0.7
87,448 - +0.6
87,448 - +0.8
87,448 - -1.3
87,448 - +0.3
87,478 - -1.0

Or see this region's nucleotide sequence