Experiment: Neomycin 0.04 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_00420 and PS417_00425 are separated by 168 nucleotides PS417_00425 and PS417_00430 are separated by 10 nucleotides PS417_00430 and PS417_00435 overlap by 4 nucleotides
PS417_00420: PS417_00420 - cytochrome C, at 82,196 to 82,822
_00420
PS417_00425: PS417_00425 - thiol:disulfide interchange protein, at 82,991 to 83,626
_00425
PS417_00430: PS417_00430 - endonuclease, at 83,637 to 84,515
_00430
PS417_00435: PS417_00435 - DeoR faimly transcriptional regulator, at 84,512 to 86,566
_00435
Position (kb) 83
84
85 Strain fitness (log2 ratio) -1
0
1 at 82.821 kb on - strand at 82.832 kb on - strand at 82.849 kb on - strand at 82.868 kb on - strand at 82.971 kb on + strand at 82.985 kb on + strand at 83.624 kb on + strand at 83.790 kb on + strand, within PS417_00430 at 83.791 kb on - strand, within PS417_00430 at 83.791 kb on - strand, within PS417_00430 at 83.791 kb on - strand, within PS417_00430 at 84.189 kb on + strand, within PS417_00430 at 84.190 kb on - strand, within PS417_00430 at 84.190 kb on - strand, within PS417_00430 at 84.190 kb on - strand, within PS417_00430 at 84.213 kb on + strand, within PS417_00430 at 84.214 kb on - strand, within PS417_00430 at 84.357 kb on - strand, within PS417_00430 at 84.357 kb on - strand, within PS417_00430 at 84.371 kb on + strand, within PS417_00430 at 84.551 kb on - strand at 84.551 kb on - strand at 84.710 kb on - strand at 85.175 kb on - strand, within PS417_00435 at 85.493 kb on + strand, within PS417_00435 at 85.493 kb on + strand, within PS417_00435 at 85.493 kb on + strand, within PS417_00435 at 85.494 kb on - strand, within PS417_00435 at 85.494 kb on - strand, within PS417_00435 at 85.494 kb on - strand, within PS417_00435 at 85.494 kb on - strand, within PS417_00435 at 85.494 kb on - strand, within PS417_00435
Per-strain Table
Position Strand Gene LocusTag Fraction Neomycin 0.04 mg/ml remove 82,821 - -1.2 82,832 - +0.4 82,849 - -0.4 82,868 - +0.6 82,971 + -0.3 82,985 + -1.5 83,624 + -0.2 83,790 + PS417_00430 0.17 +0.6 83,791 - PS417_00430 0.18 +0.5 83,791 - PS417_00430 0.18 +0.4 83,791 - PS417_00430 0.18 +0.7 84,189 + PS417_00430 0.63 +0.8 84,190 - PS417_00430 0.63 +0.1 84,190 - PS417_00430 0.63 +0.2 84,190 - PS417_00430 0.63 +1.4 84,213 + PS417_00430 0.66 +0.5 84,214 - PS417_00430 0.66 +0.9 84,357 - PS417_00430 0.82 +0.8 84,357 - PS417_00430 0.82 +0.8 84,371 + PS417_00430 0.84 +0.4 84,551 - -0.7 84,551 - -0.5 84,710 - +0.1 85,175 - PS417_00435 0.32 -0.9 85,493 + PS417_00435 0.48 -0.0 85,493 + PS417_00435 0.48 -0.8 85,493 + PS417_00435 0.48 +0.2 85,494 - PS417_00435 0.48 -0.3 85,494 - PS417_00435 0.48 -1.0 85,494 - PS417_00435 0.48 +0.1 85,494 - PS417_00435 0.48 +0.7 85,494 - PS417_00435 0.48 +0.0
Or see this region's nucleotide sequence