Experiment: Neomycin 0.04 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_27790 and PS417_27795 are separated by 201 nucleotides PS417_27795 and PS417_27800 are separated by 82 nucleotides PS417_27800 and PS417_27805 are separated by 58 nucleotides
PS417_27790: PS417_27790 - acetylglutamate kinase, at 5,993,123 to 5,994,028
_27790
PS417_27795: PS417_27795 - orotate phosphoribosyltransferase, at 5,994,230 to 5,994,874
_27795
PS417_27800: PS417_27800 - exodeoxyribonuclease III, at 5,994,957 to 5,995,736
_27800
PS417_27805: PS417_27805 - orotate phosphoribosyltransferase, at 5,995,795 to 5,996,166
_27805
Position (kb) 5994
5995 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 5993.867 kb on + strand, within PS417_27790 at 5994.027 kb on - strand at 5994.162 kb on - strand at 5994.515 kb on + strand, within PS417_27795 at 5994.891 kb on - strand at 5994.891 kb on - strand at 5994.910 kb on - strand at 5994.910 kb on - strand at 5994.925 kb on - strand at 5994.930 kb on - strand at 5994.956 kb on + strand at 5995.384 kb on - strand, within PS417_27800 at 5995.389 kb on + strand, within PS417_27800 at 5995.389 kb on + strand, within PS417_27800 at 5995.389 kb on + strand, within PS417_27800 at 5995.389 kb on + strand, within PS417_27800 at 5995.404 kb on + strand, within PS417_27800 at 5995.404 kb on + strand, within PS417_27800 at 5995.404 kb on + strand, within PS417_27800 at 5995.516 kb on - strand, within PS417_27800 at 5995.557 kb on + strand, within PS417_27800 at 5995.558 kb on - strand, within PS417_27800 at 5995.558 kb on - strand, within PS417_27800 at 5995.796 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Neomycin 0.04 mg/ml remove 5,993,867 + PS417_27790 0.82 +0.7 5,994,027 - -0.1 5,994,162 - +1.8 5,994,515 + PS417_27795 0.44 +0.7 5,994,891 - -0.5 5,994,891 - -1.3 5,994,910 - -2.5 5,994,910 - -2.6 5,994,925 - -1.2 5,994,930 - -2.8 5,994,956 + -1.4 5,995,384 - PS417_27800 0.55 -3.0 5,995,389 + PS417_27800 0.55 -0.3 5,995,389 + PS417_27800 0.55 -2.1 5,995,389 + PS417_27800 0.55 -2.2 5,995,389 + PS417_27800 0.55 -2.6 5,995,404 + PS417_27800 0.57 -1.8 5,995,404 + PS417_27800 0.57 -1.7 5,995,404 + PS417_27800 0.57 -1.4 5,995,516 - PS417_27800 0.72 -2.0 5,995,557 + PS417_27800 0.77 -2.2 5,995,558 - PS417_27800 0.77 -2.6 5,995,558 - PS417_27800 0.77 -1.5 5,995,796 + -0.2
Or see this region's nucleotide sequence