Experiment: Neomycin 0.04 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_27280 and PS417_27285 overlap by 4 nucleotides PS417_27285 and PS417_27290 are separated by 19 nucleotides PS417_27290 and PS417_27295 are separated by 138 nucleotides PS417_27295 and PS417_27300 overlap by 4 nucleotides
PS417_27280: PS417_27280 - 2-octaprenyl-6-methoxyphenyl hydroxylase, at 5,888,419 to 5,889,606
_27280
PS417_27285: PS417_27285 - Xaa-Pro aminopeptidase, at 5,889,603 to 5,890,925
_27285
PS417_27290: PS417_27290 - hypothetical protein, at 5,890,945 to 5,891,502
_27290
PS417_27295: PS417_27295 - hypothetical protein, at 5,891,641 to 5,891,850
_27295
PS417_27300: PS417_27300 - cell division protein ZapA, at 5,891,847 to 5,892,164
_27300
Position (kb) 5889
5890
5891 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 5889.761 kb on + strand, within PS417_27285 at 5889.762 kb on - strand, within PS417_27285 at 5889.762 kb on - strand, within PS417_27285 at 5889.762 kb on - strand, within PS417_27285 at 5889.763 kb on + strand, within PS417_27285 at 5889.764 kb on - strand, within PS417_27285 at 5889.764 kb on - strand, within PS417_27285 at 5889.764 kb on - strand, within PS417_27285 at 5889.830 kb on - strand, within PS417_27285 at 5889.883 kb on + strand, within PS417_27285 at 5889.883 kb on + strand, within PS417_27285 at 5889.883 kb on + strand, within PS417_27285 at 5889.883 kb on + strand, within PS417_27285 at 5889.883 kb on + strand, within PS417_27285 at 5889.884 kb on - strand, within PS417_27285 at 5889.884 kb on - strand, within PS417_27285 at 5889.884 kb on - strand, within PS417_27285 at 5889.884 kb on - strand, within PS417_27285 at 5889.893 kb on + strand, within PS417_27285 at 5889.893 kb on + strand, within PS417_27285 at 5889.895 kb on + strand, within PS417_27285 at 5889.895 kb on + strand, within PS417_27285 at 5889.895 kb on + strand, within PS417_27285 at 5889.896 kb on - strand, within PS417_27285 at 5889.896 kb on - strand, within PS417_27285 at 5890.047 kb on + strand, within PS417_27285 at 5890.048 kb on - strand, within PS417_27285 at 5890.050 kb on + strand, within PS417_27285 at 5890.051 kb on - strand, within PS417_27285 at 5890.051 kb on - strand, within PS417_27285 at 5890.051 kb on - strand, within PS417_27285 at 5890.051 kb on - strand, within PS417_27285 at 5890.051 kb on - strand, within PS417_27285 at 5890.051 kb on - strand at 5890.123 kb on + strand, within PS417_27285 at 5890.401 kb on - strand, within PS417_27285 at 5890.401 kb on - strand, within PS417_27285 at 5890.544 kb on - strand, within PS417_27285 at 5890.544 kb on - strand, within PS417_27285 at 5890.706 kb on - strand, within PS417_27285 at 5890.706 kb on - strand, within PS417_27285 at 5890.900 kb on + strand at 5890.901 kb on - strand at 5890.901 kb on - strand at 5890.901 kb on - strand at 5891.028 kb on - strand, within PS417_27290 at 5891.045 kb on + strand, within PS417_27290 at 5891.046 kb on - strand, within PS417_27290 at 5891.496 kb on + strand at 5891.497 kb on - strand at 5891.533 kb on + strand at 5891.549 kb on - strand at 5891.553 kb on + strand at 5891.554 kb on - strand at 5891.568 kb on + strand at 5891.569 kb on - strand at 5891.577 kb on + strand at 5891.687 kb on + strand, within PS417_27295 at 5891.709 kb on + strand, within PS417_27295 at 5891.729 kb on + strand, within PS417_27295 at 5891.729 kb on + strand, within PS417_27295 at 5891.732 kb on + strand, within PS417_27295 at 5891.732 kb on + strand, within PS417_27295
Per-strain Table
Position Strand Gene LocusTag Fraction Neomycin 0.04 mg/ml remove 5,889,761 + PS417_27285 0.12 -0.3 5,889,762 - PS417_27285 0.12 -1.2 5,889,762 - PS417_27285 0.12 -1.7 5,889,762 - PS417_27285 0.12 -0.6 5,889,763 + PS417_27285 0.12 -1.9 5,889,764 - PS417_27285 0.12 -1.3 5,889,764 - PS417_27285 0.12 -1.0 5,889,764 - PS417_27285 0.12 -0.1 5,889,830 - PS417_27285 0.17 -1.6 5,889,883 + PS417_27285 0.21 -1.5 5,889,883 + PS417_27285 0.21 -0.1 5,889,883 + PS417_27285 0.21 -1.0 5,889,883 + PS417_27285 0.21 -1.5 5,889,883 + PS417_27285 0.21 -2.9 5,889,884 - PS417_27285 0.21 -1.0 5,889,884 - PS417_27285 0.21 -0.4 5,889,884 - PS417_27285 0.21 -2.3 5,889,884 - PS417_27285 0.21 -1.3 5,889,893 + PS417_27285 0.22 -0.3 5,889,893 + PS417_27285 0.22 -1.1 5,889,895 + PS417_27285 0.22 +1.0 5,889,895 + PS417_27285 0.22 -1.8 5,889,895 + PS417_27285 0.22 -1.4 5,889,896 - PS417_27285 0.22 -1.9 5,889,896 - PS417_27285 0.22 -1.0 5,890,047 + PS417_27285 0.34 -1.7 5,890,048 - PS417_27285 0.34 -0.9 5,890,050 + PS417_27285 0.34 -1.1 5,890,051 - PS417_27285 0.34 +1.3 5,890,051 - PS417_27285 0.34 -1.4 5,890,051 - PS417_27285 0.34 -0.3 5,890,051 - PS417_27285 0.34 -1.2 5,890,051 - PS417_27285 0.34 -1.3 5,890,051 - +0.3 5,890,123 + PS417_27285 0.39 -0.9 5,890,401 - PS417_27285 0.60 -0.9 5,890,401 - PS417_27285 0.60 -1.6 5,890,544 - PS417_27285 0.71 -1.3 5,890,544 - PS417_27285 0.71 -1.2 5,890,706 - PS417_27285 0.83 -2.0 5,890,706 - PS417_27285 0.83 -0.6 5,890,900 + -1.4 5,890,901 - -0.8 5,890,901 - -2.2 5,890,901 - -1.4 5,891,028 - PS417_27290 0.15 -0.6 5,891,045 + PS417_27290 0.18 -0.5 5,891,046 - PS417_27290 0.18 -0.5 5,891,496 + -0.8 5,891,497 - +0.4 5,891,533 + -0.5 5,891,549 - +0.9 5,891,553 + -1.7 5,891,554 - +1.2 5,891,568 + -1.3 5,891,569 - -0.2 5,891,577 + +0.2 5,891,687 + PS417_27295 0.22 +1.8 5,891,709 + PS417_27295 0.32 +1.1 5,891,729 + PS417_27295 0.42 +0.5 5,891,729 + PS417_27295 0.42 +1.2 5,891,732 + PS417_27295 0.43 +0.8 5,891,732 + PS417_27295 0.43 +0.9
Or see this region's nucleotide sequence