Experiment: Neomycin 0.04 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_27135 and PS417_27140 are separated by 597 nucleotides PS417_27140 and PS417_27145 are separated by 220 nucleotides PS417_27145 and PS417_27150 are separated by 109 nucleotides
PS417_27135: PS417_27135 - glutamine synthetase, at 5,857,994 to 5,859,352
_27135
PS417_27140: PS417_27140 - Gamma-glutamyl-putrescine synthetase (EC 6.3.1.11) (from data) , at 5,859,950 to 5,861,326
_27140
PS417_27145: PS417_27145 - TetR family transcriptional regulator, at 5,861,547 to 5,862,188
_27145
PS417_27150: PS417_27150 - transporter, at 5,862,298 to 5,862,513
_27150
Position (kb)
5859
5860
5861
5862 Strain fitness (log2 ratio)
-1
0
1
2 at 5859.353 kb on - strand at 5859.353 kb on - strand at 5859.687 kb on + strand at 5859.688 kb on - strand at 5859.688 kb on - strand at 5860.026 kb on + strand at 5860.131 kb on + strand, within PS417_27140 at 5860.131 kb on + strand, within PS417_27140 at 5860.132 kb on - strand, within PS417_27140 at 5860.964 kb on - strand, within PS417_27140 at 5861.009 kb on - strand, within PS417_27140 at 5861.213 kb on + strand at 5861.214 kb on - strand at 5861.214 kb on - strand at 5861.225 kb on - strand at 5861.225 kb on - strand at 5861.225 kb on - strand at 5861.228 kb on + strand at 5861.300 kb on + strand at 5861.301 kb on - strand at 5861.324 kb on + strand at 5861.443 kb on + strand at 5861.516 kb on + strand at 5861.516 kb on + strand at 5861.517 kb on - strand at 5861.517 kb on - strand at 5861.517 kb on - strand at 5861.536 kb on - strand at 5861.536 kb on - strand at 5861.668 kb on - strand, within PS417_27145 at 5861.709 kb on + strand, within PS417_27145 at 5861.835 kb on + strand, within PS417_27145 at 5861.836 kb on - strand, within PS417_27145 at 5861.836 kb on - strand, within PS417_27145 at 5861.861 kb on + strand, within PS417_27145 at 5861.861 kb on + strand, within PS417_27145 at 5861.862 kb on - strand, within PS417_27145 at 5861.862 kb on - strand, within PS417_27145 at 5861.865 kb on + strand, within PS417_27145 at 5861.865 kb on + strand, within PS417_27145 at 5861.865 kb on + strand, within PS417_27145 at 5861.865 kb on + strand, within PS417_27145 at 5861.865 kb on + strand, within PS417_27145 at 5861.866 kb on - strand, within PS417_27145 at 5861.866 kb on - strand, within PS417_27145 at 5861.866 kb on - strand, within PS417_27145 at 5861.866 kb on - strand, within PS417_27145 at 5861.866 kb on - strand, within PS417_27145 at 5862.061 kb on - strand, within PS417_27145 at 5862.092 kb on + strand, within PS417_27145 at 5862.135 kb on + strand at 5862.135 kb on + strand at 5862.135 kb on + strand at 5862.135 kb on + strand at 5862.136 kb on - strand at 5862.136 kb on - strand at 5862.136 kb on - strand at 5862.136 kb on - strand at 5862.136 kb on - strand at 5862.147 kb on + strand at 5862.147 kb on + strand at 5862.147 kb on + strand at 5862.159 kb on + strand at 5862.160 kb on - strand at 5862.160 kb on - strand at 5862.160 kb on - strand at 5862.186 kb on + strand at 5862.187 kb on - strand at 5862.187 kb on - strand at 5862.196 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Neomycin 0.04 mg/ml remove 5,859,353 - +0.1 5,859,353 - +0.8 5,859,687 + -0.2 5,859,688 - +1.3 5,859,688 - -0.6 5,860,026 + +0.0 5,860,131 + PS417_27140 0.13 -0.8 5,860,131 + PS417_27140 0.13 -0.6 5,860,132 - PS417_27140 0.13 -0.3 5,860,964 - PS417_27140 0.74 -0.4 5,861,009 - PS417_27140 0.77 -1.2 5,861,213 + +0.3 5,861,214 - +0.0 5,861,214 - +0.3 5,861,225 - +0.5 5,861,225 - +0.7 5,861,225 - -0.1 5,861,228 + +0.6 5,861,300 + -0.4 5,861,301 - +0.2 5,861,324 + +0.5 5,861,443 + +0.1 5,861,516 + -0.5 5,861,516 + +0.3 5,861,517 - +1.2 5,861,517 - -0.1 5,861,517 - +0.4 5,861,536 - +0.1 5,861,536 - -0.5 5,861,668 - PS417_27145 0.19 +0.4 5,861,709 + PS417_27145 0.25 -0.3 5,861,835 + PS417_27145 0.45 -0.1 5,861,836 - PS417_27145 0.45 -0.1 5,861,836 - PS417_27145 0.45 -0.5 5,861,861 + PS417_27145 0.49 -0.1 5,861,861 + PS417_27145 0.49 +0.1 5,861,862 - PS417_27145 0.49 -1.0 5,861,862 - PS417_27145 0.49 -0.5 5,861,865 + PS417_27145 0.50 +0.1 5,861,865 + PS417_27145 0.50 +0.1 5,861,865 + PS417_27145 0.50 +0.1 5,861,865 + PS417_27145 0.50 -0.2 5,861,865 + PS417_27145 0.50 -0.5 5,861,866 - PS417_27145 0.50 -0.2 5,861,866 - PS417_27145 0.50 -0.0 5,861,866 - PS417_27145 0.50 +0.2 5,861,866 - PS417_27145 0.50 +0.1 5,861,866 - PS417_27145 0.50 -0.4 5,862,061 - PS417_27145 0.80 -0.2 5,862,092 + PS417_27145 0.85 -0.2 5,862,135 + +1.9 5,862,135 + -0.3 5,862,135 + +0.1 5,862,135 + -0.8 5,862,136 - +0.1 5,862,136 - -0.2 5,862,136 - +0.8 5,862,136 - +0.1 5,862,136 - -0.7 5,862,147 + +0.3 5,862,147 + -0.3 5,862,147 + +0.2 5,862,159 + +0.2 5,862,160 - +0.4 5,862,160 - -1.7 5,862,160 - -0.9 5,862,186 + -0.3 5,862,187 - -0.1 5,862,187 - -0.3 5,862,196 - -0.2
Or see this region's nucleotide sequence