Experiment: Neomycin 0.04 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_25105 and PS417_25110 overlap by 4 nucleotides PS417_25110 and PS417_25115 are separated by 13 nucleotides PS417_25115 and PS417_25120 are separated by 66 nucleotides PS417_25120 and PS417_25125 are separated by 56 nucleotides
PS417_25105: PS417_25105 - hypothetical protein, at 5,443,827 to 5,444,567
_25105
PS417_25110: PS417_25110 - capsular biosynthesis protein, at 5,444,564 to 5,445,289
_25110
PS417_25115: PS417_25115 - lipopolysaccharide biosynthesis protein, at 5,445,303 to 5,447,519
_25115
PS417_25120: PS417_25120 - capsular biosynthesis protein, at 5,447,586 to 5,448,125
_25120
PS417_25125: PS417_25125 - hypothetical protein, at 5,448,182 to 5,449,336
_25125
Position (kb)
5445
5446
5447
5448 Strain fitness (log2 ratio)
-1
0
1
2
3
4 at 5444.340 kb on + strand, within PS417_25105 at 5444.350 kb on - strand, within PS417_25105 at 5444.483 kb on + strand, within PS417_25105 at 5444.483 kb on + strand, within PS417_25105 at 5444.483 kb on + strand, within PS417_25105 at 5444.955 kb on - strand, within PS417_25110 at 5444.993 kb on + strand, within PS417_25110 at 5444.993 kb on + strand, within PS417_25110 at 5444.993 kb on + strand, within PS417_25110 at 5444.994 kb on - strand, within PS417_25110 at 5445.139 kb on + strand, within PS417_25110 at 5445.139 kb on + strand, within PS417_25110 at 5445.172 kb on + strand, within PS417_25110 at 5445.172 kb on + strand, within PS417_25110 at 5445.172 kb on + strand, within PS417_25110 at 5445.328 kb on + strand at 5445.337 kb on + strand at 5445.484 kb on + strand at 5445.484 kb on + strand at 5445.484 kb on + strand at 5445.484 kb on + strand at 5445.485 kb on - strand at 5445.485 kb on - strand at 5445.485 kb on - strand at 5445.485 kb on - strand at 5445.485 kb on - strand at 5445.485 kb on - strand at 5445.502 kb on + strand at 5445.503 kb on - strand at 5445.852 kb on - strand, within PS417_25115 at 5445.932 kb on + strand, within PS417_25115 at 5446.164 kb on - strand, within PS417_25115 at 5446.182 kb on - strand, within PS417_25115 at 5446.182 kb on - strand, within PS417_25115 at 5446.191 kb on + strand, within PS417_25115 at 5446.224 kb on - strand, within PS417_25115 at 5446.277 kb on - strand, within PS417_25115 at 5446.411 kb on + strand, within PS417_25115 at 5446.416 kb on - strand, within PS417_25115 at 5446.475 kb on + strand, within PS417_25115 at 5446.476 kb on - strand, within PS417_25115 at 5446.651 kb on - strand, within PS417_25115 at 5446.651 kb on - strand, within PS417_25115 at 5446.651 kb on - strand, within PS417_25115 at 5446.651 kb on - strand, within PS417_25115 at 5446.765 kb on - strand, within PS417_25115 at 5446.765 kb on - strand, within PS417_25115 at 5446.795 kb on - strand, within PS417_25115 at 5446.795 kb on - strand at 5446.965 kb on + strand, within PS417_25115 at 5446.965 kb on + strand, within PS417_25115 at 5446.965 kb on + strand, within PS417_25115 at 5446.966 kb on - strand, within PS417_25115 at 5446.966 kb on - strand, within PS417_25115 at 5446.966 kb on - strand, within PS417_25115 at 5446.972 kb on - strand, within PS417_25115 at 5446.986 kb on - strand, within PS417_25115 at 5447.059 kb on - strand, within PS417_25115 at 5447.180 kb on + strand, within PS417_25115 at 5447.181 kb on - strand, within PS417_25115 at 5447.245 kb on + strand, within PS417_25115 at 5447.414 kb on + strand at 5447.588 kb on - strand at 5447.685 kb on - strand, within PS417_25120 at 5447.807 kb on - strand, within PS417_25120 at 5447.839 kb on - strand, within PS417_25120 at 5447.857 kb on + strand, within PS417_25120 at 5448.054 kb on - strand, within PS417_25120 at 5448.257 kb on + strand at 5448.261 kb on + strand at 5448.278 kb on - strand at 5448.295 kb on - strand at 5448.307 kb on + strand, within PS417_25125 at 5448.379 kb on - strand, within PS417_25125 at 5448.465 kb on + strand, within PS417_25125 at 5448.465 kb on + strand, within PS417_25125 at 5448.510 kb on + strand, within PS417_25125
Per-strain Table
Position Strand Gene LocusTag Fraction Neomycin 0.04 mg/ml remove 5,444,340 + PS417_25105 0.69 -0.6 5,444,350 - PS417_25105 0.71 +2.5 5,444,483 + PS417_25105 0.89 +1.0 5,444,483 + PS417_25105 0.89 -0.3 5,444,483 + PS417_25105 0.89 -0.8 5,444,955 - PS417_25110 0.54 +1.2 5,444,993 + PS417_25110 0.59 -0.3 5,444,993 + PS417_25110 0.59 -0.1 5,444,993 + PS417_25110 0.59 +0.9 5,444,994 - PS417_25110 0.59 +1.9 5,445,139 + PS417_25110 0.79 -0.9 5,445,139 + PS417_25110 0.79 +0.0 5,445,172 + PS417_25110 0.84 +0.4 5,445,172 + PS417_25110 0.84 -0.7 5,445,172 + PS417_25110 0.84 +0.2 5,445,328 + +0.1 5,445,337 + -1.1 5,445,484 + +0.0 5,445,484 + +0.3 5,445,484 + -0.0 5,445,484 + -0.3 5,445,485 - +2.1 5,445,485 - +2.3 5,445,485 - +3.8 5,445,485 - +2.5 5,445,485 - +1.2 5,445,485 - +2.2 5,445,502 + +0.0 5,445,503 - +2.6 5,445,852 - PS417_25115 0.25 +0.2 5,445,932 + PS417_25115 0.28 -0.3 5,446,164 - PS417_25115 0.39 +0.3 5,446,182 - PS417_25115 0.40 +0.3 5,446,182 - PS417_25115 0.40 -0.1 5,446,191 + PS417_25115 0.40 +0.1 5,446,224 - PS417_25115 0.42 -0.8 5,446,277 - PS417_25115 0.44 +0.1 5,446,411 + PS417_25115 0.50 +0.3 5,446,416 - PS417_25115 0.50 -0.0 5,446,475 + PS417_25115 0.53 -0.3 5,446,476 - PS417_25115 0.53 -0.0 5,446,651 - PS417_25115 0.61 -0.3 5,446,651 - PS417_25115 0.61 +0.3 5,446,651 - PS417_25115 0.61 -0.1 5,446,651 - PS417_25115 0.61 -0.4 5,446,765 - PS417_25115 0.66 +0.7 5,446,765 - PS417_25115 0.66 -0.2 5,446,795 - PS417_25115 0.67 +1.0 5,446,795 - +2.9 5,446,965 + PS417_25115 0.75 -1.1 5,446,965 + PS417_25115 0.75 -0.5 5,446,965 + PS417_25115 0.75 -0.6 5,446,966 - PS417_25115 0.75 +1.6 5,446,966 - PS417_25115 0.75 -0.2 5,446,966 - PS417_25115 0.75 +0.2 5,446,972 - PS417_25115 0.75 -0.0 5,446,986 - PS417_25115 0.76 +0.7 5,447,059 - PS417_25115 0.79 -0.3 5,447,180 + PS417_25115 0.85 +1.3 5,447,181 - PS417_25115 0.85 -0.2 5,447,245 + PS417_25115 0.88 +0.3 5,447,414 + +1.2 5,447,588 - +2.2 5,447,685 - PS417_25120 0.18 +2.5 5,447,807 - PS417_25120 0.41 +1.4 5,447,839 - PS417_25120 0.47 +2.3 5,447,857 + PS417_25120 0.50 -0.2 5,448,054 - PS417_25120 0.87 -0.3 5,448,257 + -0.3 5,448,261 + -0.1 5,448,278 - +3.1 5,448,295 - +2.5 5,448,307 + PS417_25125 0.11 +0.6 5,448,379 - PS417_25125 0.17 +1.6 5,448,465 + PS417_25125 0.25 +0.3 5,448,465 + PS417_25125 0.25 +0.3 5,448,510 + PS417_25125 0.28 +0.1
Or see this region's nucleotide sequence