Experiment: Neomycin 0.04 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_24825 and PS417_24830 are separated by 92 nucleotides PS417_24830 and PS417_24835 are separated by 305 nucleotides PS417_24835 and PS417_24840 are separated by 6 nucleotides PS417_24840 and PS417_24845 are separated by 109 nucleotides
PS417_24825: PS417_24825 - ethanolamine ammonia-lyase, at 5,385,371 to 5,386,204
_24825
PS417_24830: PS417_24830 - GNAT family acetyltransferase, at 5,386,297 to 5,386,755
_24830
PS417_24835: PS417_24835 - hypothetical protein, at 5,387,061 to 5,387,717
_24835
PS417_24840: PS417_24840 - hypothetical protein, at 5,387,724 to 5,388,539
_24840
PS417_24845: PS417_24845 - inorganic pyrophosphatase, at 5,388,649 to 5,389,176
_24845
Position (kb)
5387
5388 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 5386.070 kb on + strand, within PS417_24825 at 5386.236 kb on - strand at 5386.271 kb on - strand at 5386.499 kb on - strand, within PS417_24830 at 5386.552 kb on + strand, within PS417_24830 at 5386.553 kb on - strand, within PS417_24830 at 5386.553 kb on - strand, within PS417_24830 at 5386.553 kb on - strand, within PS417_24830 at 5386.553 kb on - strand, within PS417_24830 at 5386.553 kb on - strand, within PS417_24830 at 5386.553 kb on - strand, within PS417_24830 at 5386.662 kb on + strand, within PS417_24830 at 5386.663 kb on - strand, within PS417_24830 at 5386.688 kb on - strand, within PS417_24830 at 5386.729 kb on + strand at 5386.730 kb on - strand at 5386.887 kb on + strand at 5386.887 kb on + strand at 5386.887 kb on + strand at 5386.888 kb on - strand at 5386.954 kb on - strand at 5386.975 kb on + strand at 5386.990 kb on - strand at 5386.990 kb on - strand at 5386.990 kb on - strand at 5386.990 kb on - strand at 5386.998 kb on - strand at 5387.103 kb on + strand at 5387.120 kb on - strand at 5387.127 kb on + strand, within PS417_24835 at 5387.143 kb on - strand at 5387.316 kb on + strand, within PS417_24835 at 5387.316 kb on + strand, within PS417_24835 at 5387.316 kb on + strand, within PS417_24835 at 5387.317 kb on - strand, within PS417_24835 at 5387.317 kb on - strand, within PS417_24835 at 5387.317 kb on - strand, within PS417_24835 at 5388.168 kb on + strand, within PS417_24840 at 5388.291 kb on + strand, within PS417_24840 at 5388.292 kb on - strand, within PS417_24840 at 5388.466 kb on - strand at 5388.544 kb on - strand at 5388.582 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Neomycin 0.04 mg/ml remove 5,386,070 + PS417_24825 0.84 +0.0 5,386,236 - -0.9 5,386,271 - -0.4 5,386,499 - PS417_24830 0.44 -1.8 5,386,552 + PS417_24830 0.56 -0.5 5,386,553 - PS417_24830 0.56 +0.4 5,386,553 - PS417_24830 0.56 +0.0 5,386,553 - PS417_24830 0.56 +0.1 5,386,553 - PS417_24830 0.56 -1.1 5,386,553 - PS417_24830 0.56 -0.5 5,386,553 - PS417_24830 0.56 -0.2 5,386,662 + PS417_24830 0.80 +0.9 5,386,663 - PS417_24830 0.80 +0.4 5,386,688 - PS417_24830 0.85 -0.3 5,386,729 + +0.0 5,386,730 - -1.4 5,386,887 + +1.0 5,386,887 + -0.7 5,386,887 + +0.3 5,386,888 - -0.6 5,386,954 - -1.6 5,386,975 + +0.3 5,386,990 - -3.2 5,386,990 - -3.3 5,386,990 - -2.4 5,386,990 - -1.6 5,386,998 - -3.6 5,387,103 + -1.9 5,387,120 - -1.9 5,387,127 + PS417_24835 0.10 -3.4 5,387,143 - +1.3 5,387,316 + PS417_24835 0.39 -3.3 5,387,316 + PS417_24835 0.39 -1.8 5,387,316 + PS417_24835 0.39 -2.9 5,387,317 - PS417_24835 0.39 -4.3 5,387,317 - PS417_24835 0.39 -5.1 5,387,317 - PS417_24835 0.39 -4.1 5,388,168 + PS417_24840 0.54 +0.5 5,388,291 + PS417_24840 0.69 -1.5 5,388,292 - PS417_24840 0.70 -0.9 5,388,466 - +0.2 5,388,544 - -0.8 5,388,582 + -0.4
Or see this region's nucleotide sequence