Experiment: Neomycin 0.04 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_23895 and PS417_23900 are separated by 697 nucleotides PS417_23900 and PS417_23905 are separated by 2 nucleotides PS417_23905 and PS417_23910 are separated by 307 nucleotides
PS417_23895: PS417_23895 - Fis family transcriptional regulator, at 5,170,219 to 5,171,646
_23895
PS417_23900: PS417_23900 - poly(A) polymerase, at 5,172,344 to 5,173,747
_23900
PS417_23905: PS417_23905 - 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine pyrophosphokinase, at 5,173,750 to 5,174,235
_23905
PS417_23910: PS417_23910 - 3-methyl-2-oxobutanoate hydroxymethyltransferase, at 5,174,543 to 5,175,343
_23910
Position (kb)
5172
5173
5174 Strain fitness (log2 ratio)
-1
0
1
2
3 at 5171.829 kb on + strand at 5171.835 kb on + strand at 5171.836 kb on - strand at 5172.169 kb on + strand at 5172.198 kb on + strand at 5172.199 kb on - strand at 5172.255 kb on - strand at 5172.271 kb on - strand at 5172.278 kb on - strand at 5172.388 kb on + strand at 5172.388 kb on + strand at 5172.389 kb on - strand at 5172.389 kb on - strand at 5172.512 kb on + strand, within PS417_23900 at 5172.512 kb on + strand, within PS417_23900 at 5172.655 kb on + strand, within PS417_23900 at 5172.655 kb on + strand, within PS417_23900 at 5172.836 kb on + strand, within PS417_23900 at 5172.836 kb on + strand, within PS417_23900 at 5172.836 kb on + strand, within PS417_23900 at 5172.839 kb on + strand, within PS417_23900 at 5172.839 kb on + strand, within PS417_23900 at 5172.882 kb on + strand, within PS417_23900 at 5172.882 kb on + strand, within PS417_23900 at 5172.883 kb on - strand, within PS417_23900 at 5173.089 kb on + strand, within PS417_23900 at 5173.090 kb on - strand, within PS417_23900 at 5173.090 kb on - strand, within PS417_23900 at 5173.103 kb on + strand, within PS417_23900 at 5173.184 kb on + strand, within PS417_23900 at 5173.184 kb on + strand, within PS417_23900 at 5173.196 kb on + strand, within PS417_23900 at 5173.196 kb on + strand, within PS417_23900 at 5173.762 kb on + strand at 5174.398 kb on + strand at 5174.398 kb on + strand at 5174.399 kb on - strand at 5174.400 kb on + strand at 5174.450 kb on + strand at 5174.450 kb on + strand at 5174.450 kb on + strand at 5174.451 kb on - strand at 5174.451 kb on - strand at 5174.451 kb on - strand at 5174.452 kb on + strand at 5174.453 kb on - strand at 5174.453 kb on - strand at 5174.453 kb on - strand at 5174.453 kb on - strand at 5174.453 kb on - strand at 5174.481 kb on + strand at 5174.483 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Neomycin 0.04 mg/ml remove 5,171,829 + -0.3 5,171,835 + +0.4 5,171,836 - +0.6 5,172,169 + +0.0 5,172,198 + -0.3 5,172,199 - +0.6 5,172,255 - +0.4 5,172,271 - +0.2 5,172,278 - +0.8 5,172,388 + +1.6 5,172,388 + +1.4 5,172,389 - +0.8 5,172,389 - +0.1 5,172,512 + PS417_23900 0.12 -0.5 5,172,512 + PS417_23900 0.12 +2.9 5,172,655 + PS417_23900 0.22 +2.2 5,172,655 + PS417_23900 0.22 +1.7 5,172,836 + PS417_23900 0.35 +1.8 5,172,836 + PS417_23900 0.35 +1.8 5,172,836 + PS417_23900 0.35 +2.2 5,172,839 + PS417_23900 0.35 +2.2 5,172,839 + PS417_23900 0.35 +2.0 5,172,882 + PS417_23900 0.38 +1.8 5,172,882 + PS417_23900 0.38 +1.4 5,172,883 - PS417_23900 0.38 +1.2 5,173,089 + PS417_23900 0.53 +2.1 5,173,090 - PS417_23900 0.53 +1.5 5,173,090 - PS417_23900 0.53 +1.4 5,173,103 + PS417_23900 0.54 +2.5 5,173,184 + PS417_23900 0.60 +1.4 5,173,184 + PS417_23900 0.60 +1.8 5,173,196 + PS417_23900 0.61 +2.4 5,173,196 + PS417_23900 0.61 +1.8 5,173,762 + -0.7 5,174,398 + +0.6 5,174,398 + +0.4 5,174,399 - -1.3 5,174,400 + +0.3 5,174,450 + +2.3 5,174,450 + +0.7 5,174,450 + -0.1 5,174,451 - +1.0 5,174,451 - +1.5 5,174,451 - +1.5 5,174,452 + -0.1 5,174,453 - -0.1 5,174,453 - +0.4 5,174,453 - +1.0 5,174,453 - +0.3 5,174,453 - +0.5 5,174,481 + +0.2 5,174,483 + -0.0
Or see this region's nucleotide sequence