Experiment: Neomycin 0.04 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_22400 and PS417_22405 are separated by 16 nucleotides PS417_22405 and PS417_22410 are separated by 164 nucleotides PS417_22410 and PS417_22415 are separated by 6 nucleotides PS417_22415 and PS417_22420 are separated by 55 nucleotides
PS417_22400: PS417_22400 - 7-cyano-7-deazaguanine synthase, at 4,853,692 to 4,854,366
_22400
PS417_22405: PS417_22405 - 7-carboxy-7-deazaguanine synthase, at 4,854,383 to 4,855,030
_22405
PS417_22410: PS417_22410 - hypothetical protein, at 4,855,195 to 4,856,028
_22410
PS417_22415: PS417_22415 - peptidoglycan-binding protein, at 4,856,035 to 4,856,535
_22415
PS417_22420: PS417_22420 - translocation protein TolB, at 4,856,591 to 4,857,892
_22420
Position (kb) 4855
4856
4857 Strain fitness (log2 ratio) -2
-1
0
1
2 at 4854.275 kb on + strand, within PS417_22400 at 4854.276 kb on - strand, within PS417_22400 at 4854.276 kb on - strand, within PS417_22400 at 4854.276 kb on - strand, within PS417_22400 at 4854.276 kb on - strand, within PS417_22400 at 4854.276 kb on - strand, within PS417_22400 at 4854.415 kb on + strand at 4854.415 kb on + strand at 4854.415 kb on + strand at 4854.416 kb on - strand at 4854.416 kb on - strand at 4854.416 kb on - strand at 4854.416 kb on - strand at 4854.443 kb on + strand at 4854.512 kb on + strand at 4854.512 kb on + strand, within PS417_22405 at 4854.513 kb on - strand, within PS417_22405 at 4854.513 kb on - strand, within PS417_22405 at 4854.513 kb on - strand, within PS417_22405 at 4854.570 kb on + strand, within PS417_22405 at 4854.571 kb on - strand, within PS417_22405 at 4854.829 kb on - strand, within PS417_22405 at 4854.874 kb on + strand at 4854.875 kb on - strand, within PS417_22405 at 4854.996 kb on + strand at 4855.012 kb on + strand at 4855.012 kb on + strand at 4855.013 kb on - strand at 4855.013 kb on - strand at 4855.016 kb on + strand at 4855.016 kb on + strand at 4855.016 kb on + strand at 4855.016 kb on + strand at 4855.016 kb on + strand at 4855.017 kb on - strand at 4855.017 kb on - strand at 4855.017 kb on - strand at 4855.017 kb on - strand at 4855.030 kb on + strand at 4855.030 kb on + strand at 4855.031 kb on - strand at 4855.052 kb on + strand at 4855.052 kb on + strand at 4855.052 kb on + strand at 4855.053 kb on - strand at 4855.103 kb on + strand at 4855.248 kb on - strand at 4855.309 kb on + strand, within PS417_22410 at 4855.309 kb on + strand, within PS417_22410 at 4855.310 kb on - strand, within PS417_22410 at 4855.310 kb on - strand, within PS417_22410 at 4855.325 kb on + strand, within PS417_22410 at 4855.488 kb on + strand, within PS417_22410 at 4855.548 kb on - strand, within PS417_22410 at 4855.548 kb on - strand, within PS417_22410 at 4855.548 kb on - strand, within PS417_22410 at 4855.550 kb on + strand, within PS417_22410 at 4856.005 kb on + strand at 4856.037 kb on - strand at 4856.041 kb on - strand at 4856.041 kb on - strand at 4856.602 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Neomycin 0.04 mg/ml remove 4,854,275 + PS417_22400 0.86 +0.3 4,854,276 - PS417_22400 0.87 +1.2 4,854,276 - PS417_22400 0.87 +0.4 4,854,276 - PS417_22400 0.87 +0.3 4,854,276 - PS417_22400 0.87 -1.1 4,854,276 - PS417_22400 0.87 +0.2 4,854,415 + +1.0 4,854,415 + +0.5 4,854,415 + +0.2 4,854,416 - -0.1 4,854,416 - +0.6 4,854,416 - -0.6 4,854,416 - +1.0 4,854,443 + +1.0 4,854,512 + +2.2 4,854,512 + PS417_22405 0.20 +0.2 4,854,513 - PS417_22405 0.20 +0.2 4,854,513 - PS417_22405 0.20 +0.1 4,854,513 - PS417_22405 0.20 -0.8 4,854,570 + PS417_22405 0.29 +0.9 4,854,571 - PS417_22405 0.29 +0.2 4,854,829 - PS417_22405 0.69 -0.1 4,854,874 + -0.8 4,854,875 - PS417_22405 0.76 +0.2 4,854,996 + +0.0 4,855,012 + +0.1 4,855,012 + +0.2 4,855,013 - +0.1 4,855,013 - +0.7 4,855,016 + +0.5 4,855,016 + -0.1 4,855,016 + +0.3 4,855,016 + +0.8 4,855,016 + -0.1 4,855,017 - -0.1 4,855,017 - -0.6 4,855,017 - +0.7 4,855,017 - +0.2 4,855,030 + -1.0 4,855,030 + -0.2 4,855,031 - -0.7 4,855,052 + +0.1 4,855,052 + -0.9 4,855,052 + -0.4 4,855,053 - -0.2 4,855,103 + -0.4 4,855,248 - -1.0 4,855,309 + PS417_22410 0.14 -0.3 4,855,309 + PS417_22410 0.14 -0.3 4,855,310 - PS417_22410 0.14 -0.4 4,855,310 - PS417_22410 0.14 -1.4 4,855,325 + PS417_22410 0.16 -0.5 4,855,488 + PS417_22410 0.35 -0.4 4,855,548 - PS417_22410 0.42 -0.1 4,855,548 - PS417_22410 0.42 -1.4 4,855,548 - PS417_22410 0.42 -0.7 4,855,550 + PS417_22410 0.43 -1.0 4,856,005 + -1.9 4,856,037 - -0.4 4,856,041 - -1.4 4,856,041 - -2.1 4,856,602 - -0.8
Or see this region's nucleotide sequence