Experiment: Neomycin 0.04 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_02120 and PS417_02125 are separated by 304 nucleotides PS417_02125 and PS417_02130 are separated by 484 nucleotides
PS417_02115: PS417_02115 - acyltransferase, at 471,779 to 472,591
_02115
PS417_02120: PS417_02120 - 3-oxoacyl-ACP synthase, at 472,567 to 473,286
_02120
PS417_02125: PS417_02125 - cobalamin biosynthesis protein CobQ, at 473,591 to 474,361
_02125
PS417_02130: PS417_02130 - hypothetical protein, at 474,846 to 475,064
_02130
Position (kb) 473
474
475 Strain fitness (log2 ratio) -1
0
1
2 at 473.296 kb on - strand at 473.397 kb on - strand at 473.403 kb on + strand at 473.403 kb on + strand at 473.448 kb on + strand at 473.449 kb on - strand at 473.554 kb on + strand at 473.575 kb on - strand at 473.575 kb on - strand at 473.726 kb on + strand at 473.726 kb on + strand, within PS417_02125 at 473.752 kb on + strand, within PS417_02125 at 473.755 kb on - strand, within PS417_02125 at 473.824 kb on - strand, within PS417_02125 at 473.824 kb on - strand, within PS417_02125 at 473.827 kb on + strand, within PS417_02125 at 473.828 kb on - strand, within PS417_02125 at 473.828 kb on - strand, within PS417_02125 at 473.828 kb on - strand, within PS417_02125 at 473.831 kb on + strand, within PS417_02125 at 473.832 kb on - strand, within PS417_02125 at 474.132 kb on + strand, within PS417_02125 at 474.132 kb on + strand, within PS417_02125 at 474.237 kb on - strand, within PS417_02125 at 474.237 kb on - strand, within PS417_02125 at 474.237 kb on - strand, within PS417_02125 at 474.311 kb on - strand at 474.311 kb on - strand at 474.431 kb on + strand at 474.431 kb on + strand at 474.431 kb on + strand at 474.432 kb on - strand at 474.479 kb on - strand at 474.556 kb on + strand at 474.557 kb on - strand at 474.557 kb on - strand at 474.567 kb on - strand at 474.653 kb on - strand at 474.678 kb on - strand at 474.704 kb on - strand at 474.710 kb on + strand at 474.710 kb on + strand at 474.710 kb on + strand at 474.711 kb on - strand at 474.711 kb on - strand at 474.712 kb on + strand at 474.712 kb on + strand at 474.712 kb on + strand at 474.712 kb on + strand at 474.713 kb on - strand at 474.713 kb on - strand at 474.775 kb on - strand at 474.793 kb on - strand at 474.806 kb on + strand at 474.852 kb on - strand at 474.852 kb on - strand at 475.057 kb on + strand at 475.058 kb on - strand at 475.111 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Neomycin 0.04 mg/ml remove 473,296 - +0.3 473,397 - -0.9 473,403 + +0.8 473,403 + +0.8 473,448 + -0.0 473,449 - +0.1 473,554 + +1.7 473,575 - +0.2 473,575 - -0.0 473,726 + +0.5 473,726 + PS417_02125 0.18 -1.6 473,752 + PS417_02125 0.21 -1.0 473,755 - PS417_02125 0.21 -0.2 473,824 - PS417_02125 0.30 -0.4 473,824 - PS417_02125 0.30 +0.1 473,827 + PS417_02125 0.31 -0.7 473,828 - PS417_02125 0.31 -0.4 473,828 - PS417_02125 0.31 -0.9 473,828 - PS417_02125 0.31 +0.3 473,831 + PS417_02125 0.31 -0.6 473,832 - PS417_02125 0.31 +0.8 474,132 + PS417_02125 0.70 +0.1 474,132 + PS417_02125 0.70 -0.4 474,237 - PS417_02125 0.84 -0.4 474,237 - PS417_02125 0.84 -0.5 474,237 - PS417_02125 0.84 -0.7 474,311 - -0.1 474,311 - +0.5 474,431 + -1.5 474,431 + -0.1 474,431 + -0.0 474,432 - -1.2 474,479 - +0.0 474,556 + +0.4 474,557 - +0.4 474,557 - +0.3 474,567 - +0.3 474,653 - -0.3 474,678 - -0.3 474,704 - +0.9 474,710 + -0.0 474,710 + -0.3 474,710 + +0.1 474,711 - -0.1 474,711 - +0.0 474,712 + +0.1 474,712 + +1.9 474,712 + -0.3 474,712 + +0.0 474,713 - +0.1 474,713 - -0.1 474,775 - -0.2 474,793 - -0.1 474,806 + -1.1 474,852 - -0.4 474,852 - -0.5 475,057 + +1.2 475,058 - -0.1 475,111 + -0.9
Or see this region's nucleotide sequence