Experiment: Neomycin 0.04 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_18855 and PS417_18860 are separated by 164 nucleotides PS417_18860 and PS417_18865 are separated by 82 nucleotides PS417_18865 and PS417_18870 are separated by 59 nucleotides PS417_18870 and PS417_18875 are separated by 16 nucleotides PS417_18875 and PS417_18880 are separated by 39 nucleotides
PS417_18855: PS417_18855 - peptidase, at 4,085,827 to 4,086,366
_18855
PS417_18860: PS417_18860 - transcriptional regulator, at 4,086,531 to 4,086,914
_18860
PS417_18865: PS417_18865 - hypothetical protein, at 4,086,997 to 4,087,647
_18865
PS417_18870: PS417_18870 - hypothetical protein, at 4,087,707 to 4,088,282
_18870
PS417_18875: PS417_18875 - hypothetical protein, at 4,088,299 to 4,088,589
_18875
PS417_18880: PS417_18880 - SAM-dependent methyltransferase, at 4,088,629 to 4,089,324
_18880
Position (kb) 4086
4087
4088 Strain fitness (log2 ratio) -1
0
1
2 at 4086.053 kb on + strand, within PS417_18855 at 4086.089 kb on + strand, within PS417_18855 at 4086.089 kb on + strand, within PS417_18855 at 4086.089 kb on + strand, within PS417_18855 at 4086.090 kb on - strand, within PS417_18855 at 4086.219 kb on + strand, within PS417_18855 at 4086.220 kb on - strand at 4086.220 kb on - strand, within PS417_18855 at 4086.299 kb on + strand, within PS417_18855 at 4086.299 kb on + strand, within PS417_18855 at 4086.299 kb on + strand, within PS417_18855 at 4086.299 kb on + strand, within PS417_18855 at 4086.300 kb on - strand, within PS417_18855 at 4086.346 kb on - strand at 4086.346 kb on - strand at 4086.346 kb on - strand at 4086.350 kb on - strand at 4086.452 kb on - strand at 4086.456 kb on - strand at 4086.486 kb on - strand at 4086.493 kb on - strand at 4086.493 kb on - strand at 4086.638 kb on - strand, within PS417_18860 at 4086.638 kb on - strand, within PS417_18860 at 4086.658 kb on - strand, within PS417_18860 at 4086.658 kb on - strand, within PS417_18860 at 4086.658 kb on - strand at 4086.658 kb on - strand, within PS417_18860 at 4086.658 kb on - strand, within PS417_18860 at 4086.680 kb on + strand, within PS417_18860 at 4086.839 kb on - strand, within PS417_18860 at 4086.913 kb on - strand at 4087.062 kb on + strand, within PS417_18865 at 4087.114 kb on + strand, within PS417_18865 at 4087.115 kb on - strand, within PS417_18865 at 4087.210 kb on + strand, within PS417_18865 at 4087.210 kb on + strand, within PS417_18865 at 4087.446 kb on + strand, within PS417_18865 at 4087.645 kb on + strand at 4087.646 kb on - strand at 4087.646 kb on - strand at 4087.799 kb on + strand, within PS417_18870 at 4087.818 kb on + strand, within PS417_18870 at 4087.818 kb on + strand, within PS417_18870 at 4087.818 kb on + strand, within PS417_18870 at 4087.818 kb on + strand, within PS417_18870 at 4087.819 kb on - strand, within PS417_18870 at 4087.819 kb on - strand, within PS417_18870 at 4087.819 kb on - strand, within PS417_18870 at 4087.916 kb on + strand, within PS417_18870 at 4087.916 kb on + strand, within PS417_18870 at 4087.916 kb on + strand, within PS417_18870 at 4087.916 kb on + strand, within PS417_18870 at 4087.916 kb on + strand, within PS417_18870 at 4087.917 kb on - strand, within PS417_18870 at 4087.917 kb on - strand, within PS417_18870 at 4087.917 kb on - strand, within PS417_18870 at 4087.917 kb on - strand, within PS417_18870 at 4087.917 kb on - strand, within PS417_18870 at 4087.917 kb on - strand, within PS417_18870 at 4087.917 kb on - strand at 4087.917 kb on - strand, within PS417_18870 at 4087.917 kb on - strand, within PS417_18870 at 4087.982 kb on - strand, within PS417_18870 at 4087.982 kb on - strand, within PS417_18870 at 4087.982 kb on - strand, within PS417_18870 at 4088.291 kb on - strand at 4088.300 kb on + strand at 4088.602 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Neomycin 0.04 mg/ml remove 4,086,053 + PS417_18855 0.42 +0.3 4,086,089 + PS417_18855 0.49 -0.4 4,086,089 + PS417_18855 0.49 -0.0 4,086,089 + PS417_18855 0.49 -0.5 4,086,090 - PS417_18855 0.49 +0.4 4,086,219 + PS417_18855 0.73 +1.0 4,086,220 - +1.6 4,086,220 - PS417_18855 0.73 -0.6 4,086,299 + PS417_18855 0.87 -0.1 4,086,299 + PS417_18855 0.87 -0.3 4,086,299 + PS417_18855 0.87 +1.8 4,086,299 + PS417_18855 0.87 +0.1 4,086,300 - PS417_18855 0.88 +0.5 4,086,346 - -0.1 4,086,346 - +0.1 4,086,346 - -0.1 4,086,350 - +0.6 4,086,452 - +0.5 4,086,456 - -0.6 4,086,486 - -0.5 4,086,493 - +0.8 4,086,493 - +0.3 4,086,638 - PS417_18860 0.28 +0.3 4,086,638 - PS417_18860 0.28 -1.2 4,086,658 - PS417_18860 0.33 +0.0 4,086,658 - PS417_18860 0.33 -0.1 4,086,658 - +0.0 4,086,658 - PS417_18860 0.33 -0.2 4,086,658 - PS417_18860 0.33 -0.5 4,086,680 + PS417_18860 0.39 +0.9 4,086,839 - PS417_18860 0.80 -0.2 4,086,913 - +0.0 4,087,062 + PS417_18865 0.10 +0.6 4,087,114 + PS417_18865 0.18 -0.2 4,087,115 - PS417_18865 0.18 +1.0 4,087,210 + PS417_18865 0.33 +0.3 4,087,210 + PS417_18865 0.33 +0.7 4,087,446 + PS417_18865 0.69 +0.9 4,087,645 + +0.5 4,087,646 - -0.0 4,087,646 - -0.2 4,087,799 + PS417_18870 0.16 -1.0 4,087,818 + PS417_18870 0.19 +0.2 4,087,818 + PS417_18870 0.19 +0.1 4,087,818 + PS417_18870 0.19 +0.7 4,087,818 + PS417_18870 0.19 -0.3 4,087,819 - PS417_18870 0.19 +0.9 4,087,819 - PS417_18870 0.19 +0.1 4,087,819 - PS417_18870 0.19 +0.6 4,087,916 + PS417_18870 0.36 +0.4 4,087,916 + PS417_18870 0.36 +0.2 4,087,916 + PS417_18870 0.36 -0.1 4,087,916 + PS417_18870 0.36 -0.2 4,087,916 + PS417_18870 0.36 -0.1 4,087,917 - PS417_18870 0.36 +0.0 4,087,917 - PS417_18870 0.36 +0.5 4,087,917 - PS417_18870 0.36 -0.2 4,087,917 - PS417_18870 0.36 +0.2 4,087,917 - PS417_18870 0.36 -0.4 4,087,917 - PS417_18870 0.36 -0.4 4,087,917 - +1.6 4,087,917 - PS417_18870 0.36 +0.6 4,087,917 - PS417_18870 0.36 +0.3 4,087,982 - PS417_18870 0.48 -0.6 4,087,982 - PS417_18870 0.48 +0.4 4,087,982 - PS417_18870 0.48 -0.3 4,088,291 - +0.3 4,088,300 + -1.3 4,088,602 - +0.5
Or see this region's nucleotide sequence