Experiment: Neomycin 0.04 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_17265 and PS417_17270 are separated by 207 nucleotides PS417_17270 and PS417_17275 are separated by 156 nucleotides
PS417_17265: PS417_17265 - methenyltetrahydrofolate cyclohydrolase, at 3,769,914 to 3,770,768
_17265
PS417_17270: PS417_17270 - D-alanyl-D-alanine endopeptidase, at 3,770,976 to 3,771,899
_17270
PS417_17275: PS417_17275 - hypothetical protein, at 3,772,056 to 3,772,688
_17275
Position (kb)
3770
3771
3772 Strain fitness (log2 ratio)
-1
0
1 at 3770.814 kb on - strand at 3770.869 kb on + strand at 3770.899 kb on - strand at 3771.008 kb on - strand at 3771.008 kb on - strand at 3771.008 kb on - strand at 3771.132 kb on + strand, within PS417_17270 at 3771.132 kb on + strand, within PS417_17270 at 3771.229 kb on - strand, within PS417_17270 at 3771.229 kb on - strand, within PS417_17270 at 3771.229 kb on - strand, within PS417_17270 at 3771.450 kb on + strand, within PS417_17270 at 3771.450 kb on + strand, within PS417_17270 at 3771.451 kb on - strand, within PS417_17270 at 3771.594 kb on + strand, within PS417_17270 at 3771.913 kb on - strand at 3771.955 kb on - strand at 3772.318 kb on + strand, within PS417_17275 at 3772.318 kb on + strand, within PS417_17275 at 3772.318 kb on + strand, within PS417_17275 at 3772.319 kb on - strand, within PS417_17275 at 3772.418 kb on - strand, within PS417_17275 at 3772.432 kb on + strand, within PS417_17275 at 3772.499 kb on + strand, within PS417_17275 at 3772.500 kb on - strand, within PS417_17275 at 3772.574 kb on - strand, within PS417_17275 at 3772.761 kb on + strand at 3772.761 kb on + strand at 3772.762 kb on - strand at 3772.776 kb on + strand at 3772.830 kb on + strand at 3772.875 kb on - strand at 3772.875 kb on - strand at 3772.890 kb on - strand at 3772.891 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Neomycin 0.04 mg/ml remove 3,770,814 - -0.1 3,770,869 + -0.1 3,770,899 - -0.3 3,771,008 - -0.6 3,771,008 - +0.5 3,771,008 - -0.0 3,771,132 + PS417_17270 0.17 +0.2 3,771,132 + PS417_17270 0.17 -0.5 3,771,229 - PS417_17270 0.27 -1.0 3,771,229 - PS417_17270 0.27 -1.1 3,771,229 - PS417_17270 0.27 -1.2 3,771,450 + PS417_17270 0.51 +0.9 3,771,450 + PS417_17270 0.51 -0.1 3,771,451 - PS417_17270 0.51 -0.5 3,771,594 + PS417_17270 0.67 -1.3 3,771,913 - +0.5 3,771,955 - +1.3 3,772,318 + PS417_17275 0.41 -0.2 3,772,318 + PS417_17275 0.41 -0.4 3,772,318 + PS417_17275 0.41 -0.3 3,772,319 - PS417_17275 0.42 -0.5 3,772,418 - PS417_17275 0.57 -1.1 3,772,432 + PS417_17275 0.59 -0.6 3,772,499 + PS417_17275 0.70 -0.4 3,772,500 - PS417_17275 0.70 -0.2 3,772,574 - PS417_17275 0.82 -0.0 3,772,761 + +1.5 3,772,761 + -0.5 3,772,762 - +1.1 3,772,776 + -0.8 3,772,830 + +1.3 3,772,875 - -0.4 3,772,875 - -0.9 3,772,890 - -0.3 3,772,891 + +0.7
Or see this region's nucleotide sequence