Experiment: Neomycin 0.04 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_17165 and PS417_17170 are separated by 214 nucleotides PS417_17170 and PS417_17175 overlap by 4 nucleotides
PS417_17165: PS417_17165 - 2,4-dienoyl-CoA reductase, at 3,747,487 to 3,749,526
_17165
PS417_17170: PS417_17170 - carbon-nitrogen hydrolase, at 3,749,741 to 3,750,871
_17170
PS417_17175: PS417_17175 - AraC family transcriptional regulator, at 3,750,868 to 3,751,896
_17175
Position (kb)
3749
3750
3751 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3748.782 kb on + strand, within PS417_17165 at 3748.881 kb on + strand, within PS417_17165 at 3748.881 kb on + strand, within PS417_17165 at 3748.882 kb on - strand, within PS417_17165 at 3748.959 kb on + strand, within PS417_17165 at 3749.018 kb on + strand, within PS417_17165 at 3749.052 kb on - strand, within PS417_17165 at 3749.312 kb on + strand at 3749.312 kb on + strand, within PS417_17165 at 3749.313 kb on - strand, within PS417_17165 at 3749.313 kb on - strand, within PS417_17165 at 3749.313 kb on - strand, within PS417_17165 at 3749.588 kb on + strand at 3749.589 kb on - strand at 3749.602 kb on + strand at 3749.602 kb on + strand at 3749.645 kb on + strand at 3749.646 kb on - strand at 3749.700 kb on + strand at 3749.701 kb on - strand at 3749.834 kb on + strand at 3749.834 kb on + strand at 3749.834 kb on + strand at 3749.834 kb on + strand at 3749.851 kb on + strand at 3749.851 kb on + strand at 3750.080 kb on + strand, within PS417_17170 at 3750.081 kb on - strand, within PS417_17170 at 3750.192 kb on - strand, within PS417_17170 at 3750.192 kb on - strand, within PS417_17170 at 3750.227 kb on + strand, within PS417_17170 at 3750.347 kb on + strand, within PS417_17170 at 3750.347 kb on + strand, within PS417_17170 at 3750.347 kb on + strand, within PS417_17170 at 3750.347 kb on + strand, within PS417_17170 at 3750.347 kb on + strand, within PS417_17170 at 3750.347 kb on + strand, within PS417_17170 at 3750.348 kb on - strand, within PS417_17170 at 3750.348 kb on - strand, within PS417_17170 at 3750.348 kb on - strand at 3750.350 kb on - strand, within PS417_17170 at 3750.419 kb on + strand, within PS417_17170 at 3750.420 kb on - strand, within PS417_17170 at 3750.436 kb on + strand, within PS417_17170 at 3750.498 kb on + strand, within PS417_17170 at 3750.498 kb on + strand, within PS417_17170 at 3750.499 kb on - strand, within PS417_17170 at 3750.700 kb on + strand, within PS417_17170 at 3750.701 kb on - strand, within PS417_17170 at 3750.859 kb on + strand at 3751.285 kb on + strand, within PS417_17175 at 3751.286 kb on - strand, within PS417_17175 at 3751.399 kb on + strand at 3751.526 kb on + strand, within PS417_17175 at 3751.527 kb on - strand, within PS417_17175 at 3751.527 kb on - strand, within PS417_17175 at 3751.753 kb on + strand, within PS417_17175 at 3751.753 kb on + strand, within PS417_17175 at 3751.792 kb on + strand, within PS417_17175 at 3751.792 kb on + strand, within PS417_17175 at 3751.792 kb on + strand, within PS417_17175
Per-strain Table
Position Strand Gene LocusTag Fraction Neomycin 0.04 mg/ml remove 3,748,782 + PS417_17165 0.63 +0.0 3,748,881 + PS417_17165 0.68 +0.7 3,748,881 + PS417_17165 0.68 +0.6 3,748,882 - PS417_17165 0.68 -0.4 3,748,959 + PS417_17165 0.72 +0.4 3,749,018 + PS417_17165 0.75 +0.3 3,749,052 - PS417_17165 0.77 +0.4 3,749,312 + +0.3 3,749,312 + PS417_17165 0.89 +0.8 3,749,313 - PS417_17165 0.90 -0.0 3,749,313 - PS417_17165 0.90 -0.4 3,749,313 - PS417_17165 0.90 -0.4 3,749,588 + +1.7 3,749,589 - -0.4 3,749,602 + +0.3 3,749,602 + +0.3 3,749,645 + +0.7 3,749,646 - -0.1 3,749,700 + -0.5 3,749,701 - -2.4 3,749,834 + -2.0 3,749,834 + -2.5 3,749,834 + -2.3 3,749,834 + -2.0 3,749,851 + -2.5 3,749,851 + -1.6 3,750,080 + PS417_17170 0.30 -2.3 3,750,081 - PS417_17170 0.30 -0.7 3,750,192 - PS417_17170 0.40 -1.4 3,750,192 - PS417_17170 0.40 -1.7 3,750,227 + PS417_17170 0.43 -1.8 3,750,347 + PS417_17170 0.54 -1.5 3,750,347 + PS417_17170 0.54 -1.8 3,750,347 + PS417_17170 0.54 -1.5 3,750,347 + PS417_17170 0.54 -1.7 3,750,347 + PS417_17170 0.54 -2.9 3,750,347 + PS417_17170 0.54 -2.0 3,750,348 - PS417_17170 0.54 -2.3 3,750,348 - PS417_17170 0.54 -0.6 3,750,348 - +0.9 3,750,350 - PS417_17170 0.54 -1.7 3,750,419 + PS417_17170 0.60 -1.0 3,750,420 - PS417_17170 0.60 -3.6 3,750,436 + PS417_17170 0.61 -1.8 3,750,498 + PS417_17170 0.67 -3.9 3,750,498 + PS417_17170 0.67 -2.5 3,750,499 - PS417_17170 0.67 -0.7 3,750,700 + PS417_17170 0.85 -2.5 3,750,701 - PS417_17170 0.85 -1.9 3,750,859 + -2.1 3,751,285 + PS417_17175 0.41 -0.3 3,751,286 - PS417_17175 0.41 +0.5 3,751,399 + +2.2 3,751,526 + PS417_17175 0.64 +1.1 3,751,527 - PS417_17175 0.64 +1.0 3,751,527 - PS417_17175 0.64 -0.1 3,751,753 + PS417_17175 0.86 +0.6 3,751,753 + PS417_17175 0.86 +0.6 3,751,792 + PS417_17175 0.90 -0.1 3,751,792 + PS417_17175 0.90 -0.6 3,751,792 + PS417_17175 0.90 +0.8
Or see this region's nucleotide sequence