Experiment: Neomycin 0.04 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_01665 and PS417_01670 are separated by 206 nucleotides PS417_01670 and PS417_01675 are separated by 238 nucleotides PS417_01675 and PS417_01680 are separated by 200 nucleotides
PS417_01665: PS417_01665 - GTP-binding protein TypA, at 365,500 to 367,320
_01665
PS417_01670: PS417_01670 - Fe-S-oxidoreductase, at 367,527 to 367,961
_01670
PS417_01675: PS417_01675 - maltodextrin phosphorylase, at 368,200 to 370,650
_01675
PS417_01680: PS417_01680 - membrane protein, at 370,851 to 373,922
_01680
Position (kb) 368
369
370
371 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4
5 at 367.323 kb on + strand at 367.536 kb on - strand at 367.582 kb on + strand, within PS417_01670 at 367.652 kb on + strand, within PS417_01670 at 367.652 kb on + strand, within PS417_01670 at 367.653 kb on - strand, within PS417_01670 at 367.653 kb on - strand, within PS417_01670 at 367.653 kb on - strand, within PS417_01670 at 367.671 kb on - strand, within PS417_01670 at 367.817 kb on + strand, within PS417_01670 at 367.818 kb on - strand, within PS417_01670 at 367.818 kb on - strand, within PS417_01670 at 367.892 kb on + strand, within PS417_01670 at 367.892 kb on + strand, within PS417_01670 at 367.893 kb on - strand, within PS417_01670 at 367.904 kb on - strand, within PS417_01670 at 368.062 kb on - strand at 368.104 kb on + strand at 368.105 kb on - strand at 368.138 kb on + strand at 368.158 kb on - strand at 368.164 kb on + strand at 368.165 kb on - strand at 368.177 kb on + strand at 368.178 kb on - strand at 368.178 kb on - strand at 368.178 kb on - strand at 368.268 kb on + strand at 368.268 kb on + strand at 368.386 kb on + strand at 368.410 kb on + strand at 368.411 kb on - strand at 368.450 kb on - strand, within PS417_01675 at 368.854 kb on + strand, within PS417_01675 at 369.067 kb on + strand, within PS417_01675 at 369.068 kb on - strand, within PS417_01675 at 369.364 kb on + strand, within PS417_01675 at 369.365 kb on - strand, within PS417_01675 at 369.365 kb on - strand, within PS417_01675 at 369.426 kb on - strand, within PS417_01675 at 369.612 kb on + strand, within PS417_01675 at 369.613 kb on - strand, within PS417_01675 at 369.632 kb on - strand, within PS417_01675 at 369.865 kb on - strand, within PS417_01675 at 369.875 kb on - strand, within PS417_01675 at 370.099 kb on + strand, within PS417_01675 at 370.284 kb on + strand, within PS417_01675 at 370.668 kb on - strand at 370.668 kb on - strand at 370.729 kb on + strand at 370.804 kb on + strand at 370.804 kb on + strand at 370.804 kb on + strand at 370.805 kb on - strand at 370.805 kb on - strand at 370.807 kb on + strand at 370.807 kb on + strand at 370.808 kb on - strand at 370.808 kb on - strand at 370.808 kb on - strand at 370.838 kb on - strand at 371.484 kb on - strand, within PS417_01680 at 371.526 kb on + strand at 371.527 kb on - strand, within PS417_01680
Per-strain Table
Position Strand Gene LocusTag Fraction Neomycin 0.04 mg/ml remove 367,323 + +0.3 367,536 - +0.3 367,582 + PS417_01670 0.13 +0.1 367,652 + PS417_01670 0.29 +0.0 367,652 + PS417_01670 0.29 -1.2 367,653 - PS417_01670 0.29 -0.0 367,653 - PS417_01670 0.29 +0.1 367,653 - PS417_01670 0.29 +0.4 367,671 - PS417_01670 0.33 +0.5 367,817 + PS417_01670 0.67 -0.9 367,818 - PS417_01670 0.67 -0.9 367,818 - PS417_01670 0.67 +0.0 367,892 + PS417_01670 0.84 +1.3 367,892 + PS417_01670 0.84 +0.3 367,893 - PS417_01670 0.84 +0.4 367,904 - PS417_01670 0.87 -1.0 368,062 - +1.3 368,104 + +0.2 368,105 - +0.1 368,138 + -1.0 368,158 - +0.3 368,164 + -0.1 368,165 - -0.1 368,177 + -0.8 368,178 - +0.8 368,178 - -0.9 368,178 - +0.1 368,268 + +4.3 368,268 + +0.1 368,386 + -0.8 368,410 + +0.2 368,411 - +0.1 368,450 - PS417_01675 0.10 -0.1 368,854 + PS417_01675 0.27 +1.3 369,067 + PS417_01675 0.35 +0.0 369,068 - PS417_01675 0.35 +0.4 369,364 + PS417_01675 0.47 -0.1 369,365 - PS417_01675 0.48 +0.5 369,365 - PS417_01675 0.48 +0.1 369,426 - PS417_01675 0.50 -0.3 369,612 + PS417_01675 0.58 +0.8 369,613 - PS417_01675 0.58 -0.3 369,632 - PS417_01675 0.58 +0.2 369,865 - PS417_01675 0.68 +5.0 369,875 - PS417_01675 0.68 +0.2 370,099 + PS417_01675 0.77 -1.4 370,284 + PS417_01675 0.85 -0.0 370,668 - -0.8 370,668 - -1.9 370,729 + +0.0 370,804 + -1.6 370,804 + +1.2 370,804 + -0.8 370,805 - +0.3 370,805 - -1.3 370,807 + -0.6 370,807 + -1.1 370,808 - +0.3 370,808 - +0.1 370,808 - -0.1 370,838 - +0.9 371,484 - PS417_01680 0.21 -1.5 371,526 + -1.3 371,527 - PS417_01680 0.22 -0.2
Or see this region's nucleotide sequence