Experiment: Neomycin 0.04 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_16390 and PS417_16395 are separated by 115 nucleotides PS417_16395 and PS417_16400 overlap by 4 nucleotides PS417_16400 and PS417_16405 overlap by 17 nucleotides PS417_16405 and PS417_16410 are separated by 88 nucleotides
PS417_16390: PS417_16390 - peroxiredoxin, at 3,586,240 to 3,586,701
_16390
PS417_16395: PS417_16395 - arsenate reductase, at 3,586,817 to 3,587,170
_16395
PS417_16400: PS417_16400 - NAD(P)H-quinone oxidoreductase, at 3,587,167 to 3,587,781
_16400
PS417_16405: PS417_16405 - membrane protein, at 3,587,765 to 3,588,181
_16405
PS417_16410: PS417_16410 - 3-methyladenine DNA glycosylase, at 3,588,270 to 3,588,941
_16410
Position (kb)
3587
3588 Strain fitness (log2 ratio)
-1
0
1
2
3 at 3586.174 kb on + strand at 3586.175 kb on - strand at 3586.175 kb on - strand at 3586.176 kb on + strand at 3586.176 kb on + strand at 3586.176 kb on + strand at 3586.177 kb on - strand at 3586.309 kb on + strand, within PS417_16390 at 3586.309 kb on + strand, within PS417_16390 at 3586.310 kb on - strand, within PS417_16390 at 3586.334 kb on + strand, within PS417_16390 at 3586.334 kb on + strand, within PS417_16390 at 3586.334 kb on + strand, within PS417_16390 at 3586.334 kb on + strand, within PS417_16390 at 3586.335 kb on - strand, within PS417_16390 at 3586.335 kb on - strand, within PS417_16390 at 3586.480 kb on + strand, within PS417_16390 at 3586.562 kb on + strand, within PS417_16390 at 3586.604 kb on - strand, within PS417_16390 at 3586.741 kb on - strand at 3586.784 kb on + strand at 3586.784 kb on + strand at 3586.784 kb on + strand at 3586.785 kb on - strand at 3586.796 kb on + strand at 3586.992 kb on + strand, within PS417_16395 at 3587.012 kb on + strand, within PS417_16395 at 3587.012 kb on + strand, within PS417_16395 at 3587.012 kb on + strand, within PS417_16395 at 3587.179 kb on + strand at 3587.179 kb on + strand at 3587.180 kb on - strand at 3587.195 kb on - strand at 3587.347 kb on + strand, within PS417_16400 at 3587.347 kb on + strand, within PS417_16400 at 3587.435 kb on - strand, within PS417_16400 at 3587.779 kb on + strand at 3587.880 kb on - strand, within PS417_16405 at 3587.880 kb on - strand, within PS417_16405 at 3587.880 kb on - strand, within PS417_16405 at 3587.880 kb on - strand, within PS417_16405 at 3587.882 kb on + strand, within PS417_16405 at 3587.882 kb on + strand, within PS417_16405 at 3587.882 kb on + strand, within PS417_16405 at 3588.023 kb on + strand, within PS417_16405 at 3588.051 kb on - strand, within PS417_16405 at 3588.079 kb on - strand, within PS417_16405 at 3588.125 kb on + strand, within PS417_16405 at 3588.125 kb on + strand, within PS417_16405 at 3588.126 kb on - strand, within PS417_16405 at 3588.126 kb on - strand, within PS417_16405 at 3588.285 kb on - strand at 3588.285 kb on - strand at 3588.286 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Neomycin 0.04 mg/ml remove 3,586,174 + +0.1 3,586,175 - -0.7 3,586,175 - +1.1 3,586,176 + -0.4 3,586,176 + -0.4 3,586,176 + +1.1 3,586,177 - +0.9 3,586,309 + PS417_16390 0.15 -1.0 3,586,309 + PS417_16390 0.15 -0.9 3,586,310 - PS417_16390 0.15 -0.4 3,586,334 + PS417_16390 0.20 -0.8 3,586,334 + PS417_16390 0.20 -1.5 3,586,334 + PS417_16390 0.20 -0.7 3,586,334 + PS417_16390 0.20 -1.3 3,586,335 - PS417_16390 0.21 -0.3 3,586,335 - PS417_16390 0.21 -0.4 3,586,480 + PS417_16390 0.52 -1.2 3,586,562 + PS417_16390 0.70 -1.1 3,586,604 - PS417_16390 0.79 -0.3 3,586,741 - +0.9 3,586,784 + -0.3 3,586,784 + -1.0 3,586,784 + -0.8 3,586,785 - -1.1 3,586,796 + +0.2 3,586,992 + PS417_16395 0.49 +3.1 3,587,012 + PS417_16395 0.55 +1.0 3,587,012 + PS417_16395 0.55 +0.3 3,587,012 + PS417_16395 0.55 -0.3 3,587,179 + +0.1 3,587,179 + +0.2 3,587,180 - +0.8 3,587,195 - -0.2 3,587,347 + PS417_16400 0.29 -0.7 3,587,347 + PS417_16400 0.29 -0.1 3,587,435 - PS417_16400 0.44 -1.4 3,587,779 + -0.0 3,587,880 - PS417_16405 0.28 -0.2 3,587,880 - PS417_16405 0.28 -0.2 3,587,880 - PS417_16405 0.28 -0.3 3,587,880 - PS417_16405 0.28 -0.4 3,587,882 + PS417_16405 0.28 -0.2 3,587,882 + PS417_16405 0.28 +0.3 3,587,882 + PS417_16405 0.28 -1.6 3,588,023 + PS417_16405 0.62 -0.6 3,588,051 - PS417_16405 0.69 +0.1 3,588,079 - PS417_16405 0.75 -0.1 3,588,125 + PS417_16405 0.86 +0.1 3,588,125 + PS417_16405 0.86 -0.2 3,588,126 - PS417_16405 0.87 -0.3 3,588,126 - PS417_16405 0.87 -0.2 3,588,285 - -0.3 3,588,285 - -0.1 3,588,286 + -0.9
Or see this region's nucleotide sequence