Experiment: Neomycin 0.04 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_12210 and PS417_12215 are separated by 211 nucleotides PS417_12215 and PS417_12220 are separated by 98 nucleotides PS417_12220 and PS417_12225 are separated by 65 nucleotides
PS417_12210: PS417_12210 - ABC transporter substrate-binding protein, at 2,653,359 to 2,655,188
_12210
PS417_12215: PS417_12215 - lytic transglycosylase, at 2,655,400 to 2,656,815
_12215
PS417_12220: PS417_12220 - hydroxyacylglutathione hydrolase, at 2,656,914 to 2,657,681
_12220
PS417_12225: PS417_12225 - SAM-dependent methyltransferase, at 2,657,747 to 2,658,505
_12225
Position (kb)
2655
2656
2657 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2654.407 kb on + strand, within PS417_12210 at 2654.408 kb on - strand, within PS417_12210 at 2654.408 kb on - strand, within PS417_12210 at 2654.408 kb on - strand, within PS417_12210 at 2654.408 kb on - strand, within PS417_12210 at 2654.414 kb on - strand, within PS417_12210 at 2654.936 kb on - strand, within PS417_12210 at 2655.108 kb on - strand at 2655.108 kb on - strand at 2655.162 kb on + strand at 2655.162 kb on + strand at 2655.163 kb on - strand at 2655.163 kb on - strand at 2655.163 kb on - strand at 2655.165 kb on + strand at 2655.165 kb on + strand at 2655.169 kb on - strand at 2655.169 kb on - strand at 2655.401 kb on + strand at 2655.401 kb on + strand at 2655.402 kb on - strand at 2655.513 kb on - strand at 2655.539 kb on + strand at 2655.540 kb on - strand at 2655.540 kb on - strand at 2655.716 kb on + strand, within PS417_12215 at 2655.716 kb on + strand, within PS417_12215 at 2655.717 kb on - strand, within PS417_12215 at 2655.726 kb on + strand, within PS417_12215 at 2656.028 kb on + strand, within PS417_12215 at 2656.080 kb on - strand, within PS417_12215 at 2656.185 kb on - strand, within PS417_12215 at 2656.289 kb on + strand, within PS417_12215 at 2656.289 kb on + strand, within PS417_12215 at 2656.289 kb on + strand, within PS417_12215 at 2656.346 kb on - strand, within PS417_12215 at 2656.370 kb on + strand, within PS417_12215 at 2656.385 kb on + strand, within PS417_12215 at 2656.458 kb on - strand, within PS417_12215 at 2656.458 kb on - strand, within PS417_12215 at 2656.587 kb on + strand, within PS417_12215 at 2656.894 kb on - strand at 2657.323 kb on + strand, within PS417_12220 at 2657.360 kb on + strand, within PS417_12220 at 2657.360 kb on + strand, within PS417_12220 at 2657.362 kb on + strand, within PS417_12220 at 2657.628 kb on + strand at 2657.642 kb on - strand at 2657.642 kb on - strand at 2657.676 kb on + strand at 2657.677 kb on - strand at 2657.677 kb on - strand at 2657.718 kb on + strand at 2657.718 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Neomycin 0.04 mg/ml remove 2,654,407 + PS417_12210 0.57 +0.1 2,654,408 - PS417_12210 0.57 -0.1 2,654,408 - PS417_12210 0.57 -0.1 2,654,408 - PS417_12210 0.57 +0.3 2,654,408 - PS417_12210 0.57 -0.5 2,654,414 - PS417_12210 0.58 -2.0 2,654,936 - PS417_12210 0.86 -0.2 2,655,108 - -0.8 2,655,108 - -0.6 2,655,162 + -0.8 2,655,162 + -0.4 2,655,163 - -0.2 2,655,163 - -0.3 2,655,163 - -0.4 2,655,165 + -0.9 2,655,165 + -0.6 2,655,169 - +0.2 2,655,169 - -2.5 2,655,401 + -0.5 2,655,401 + -1.1 2,655,402 - -0.4 2,655,513 - -1.0 2,655,539 + -2.1 2,655,540 - -0.7 2,655,540 - -0.9 2,655,716 + PS417_12215 0.22 -1.1 2,655,716 + PS417_12215 0.22 -1.9 2,655,717 - PS417_12215 0.22 -0.7 2,655,726 + PS417_12215 0.23 +0.4 2,656,028 + PS417_12215 0.44 -2.3 2,656,080 - PS417_12215 0.48 -1.4 2,656,185 - PS417_12215 0.55 -2.8 2,656,289 + PS417_12215 0.63 -0.2 2,656,289 + PS417_12215 0.63 -0.8 2,656,289 + PS417_12215 0.63 -1.8 2,656,346 - PS417_12215 0.67 -2.1 2,656,370 + PS417_12215 0.69 -2.5 2,656,385 + PS417_12215 0.70 -2.5 2,656,458 - PS417_12215 0.75 -1.4 2,656,458 - PS417_12215 0.75 -1.6 2,656,587 + PS417_12215 0.84 +0.7 2,656,894 - -1.0 2,657,323 + PS417_12220 0.53 -0.8 2,657,360 + PS417_12220 0.58 -0.7 2,657,360 + PS417_12220 0.58 -0.6 2,657,362 + PS417_12220 0.58 -0.8 2,657,628 + +0.5 2,657,642 - -0.3 2,657,642 - -0.2 2,657,676 + -0.3 2,657,677 - -0.4 2,657,677 - -0.5 2,657,718 + +0.2 2,657,718 + -0.2
Or see this region's nucleotide sequence