Experiment: Neomycin 0.04 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_10830 and PS417_10835 are separated by 136 nucleotides PS417_10835 and PS417_10840 are separated by 19 nucleotides PS417_10840 and PS417_10845 overlap by 12 nucleotides
PS417_10830: PS417_10830 - LysR family transcriptional regulator, at 2,315,398 to 2,316,315
_10830
PS417_10835: PS417_10835 - major facilitator transporter, at 2,316,452 to 2,317,768
_10835
PS417_10840: PS417_10840 - amidase, at 2,317,788 to 2,318,561
_10840
PS417_10845: PS417_10845 - hypothetical protein, at 2,318,550 to 2,318,858
_10845
Position (kb)
2316
2317
2318 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2316.451 kb on + strand at 2316.452 kb on - strand at 2316.851 kb on + strand, within PS417_10835 at 2316.851 kb on + strand, within PS417_10835 at 2316.852 kb on - strand, within PS417_10835 at 2316.854 kb on + strand, within PS417_10835 at 2316.854 kb on + strand, within PS417_10835 at 2316.869 kb on + strand, within PS417_10835 at 2317.055 kb on + strand, within PS417_10835 at 2317.055 kb on + strand, within PS417_10835 at 2317.056 kb on - strand, within PS417_10835 at 2317.165 kb on + strand, within PS417_10835 at 2317.188 kb on + strand, within PS417_10835 at 2317.188 kb on + strand, within PS417_10835 at 2317.189 kb on - strand, within PS417_10835 at 2317.189 kb on - strand, within PS417_10835 at 2317.190 kb on + strand, within PS417_10835 at 2317.191 kb on - strand, within PS417_10835 at 2317.232 kb on + strand, within PS417_10835 at 2317.320 kb on - strand, within PS417_10835 at 2317.339 kb on + strand, within PS417_10835 at 2317.343 kb on + strand, within PS417_10835 at 2317.343 kb on + strand, within PS417_10835 at 2317.343 kb on + strand, within PS417_10835 at 2317.343 kb on + strand, within PS417_10835 at 2317.518 kb on + strand, within PS417_10835 at 2317.583 kb on - strand, within PS417_10835 at 2317.583 kb on - strand, within PS417_10835 at 2317.752 kb on - strand at 2317.752 kb on - strand at 2317.795 kb on + strand at 2317.796 kb on - strand at 2317.797 kb on + strand at 2317.797 kb on + strand at 2317.798 kb on - strand at 2317.981 kb on + strand, within PS417_10840 at 2317.981 kb on + strand, within PS417_10840 at 2318.209 kb on + strand, within PS417_10840 at 2318.494 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Neomycin 0.04 mg/ml remove 2,316,451 + +0.1 2,316,452 - +0.3 2,316,851 + PS417_10835 0.30 -0.1 2,316,851 + PS417_10835 0.30 -0.6 2,316,852 - PS417_10835 0.30 -1.1 2,316,854 + PS417_10835 0.31 +0.2 2,316,854 + PS417_10835 0.31 -0.1 2,316,869 + PS417_10835 0.32 -0.4 2,317,055 + PS417_10835 0.46 -0.7 2,317,055 + PS417_10835 0.46 -2.9 2,317,056 - PS417_10835 0.46 +1.2 2,317,165 + PS417_10835 0.54 -0.3 2,317,188 + PS417_10835 0.56 +0.0 2,317,188 + PS417_10835 0.56 +0.3 2,317,189 - PS417_10835 0.56 -1.7 2,317,189 - PS417_10835 0.56 -1.4 2,317,190 + PS417_10835 0.56 -1.5 2,317,191 - PS417_10835 0.56 -0.4 2,317,232 + PS417_10835 0.59 -0.1 2,317,320 - PS417_10835 0.66 +0.5 2,317,339 + PS417_10835 0.67 -0.3 2,317,343 + PS417_10835 0.68 +0.1 2,317,343 + PS417_10835 0.68 +0.1 2,317,343 + PS417_10835 0.68 -2.4 2,317,343 + PS417_10835 0.68 -0.2 2,317,518 + PS417_10835 0.81 -0.4 2,317,583 - PS417_10835 0.86 +0.5 2,317,583 - PS417_10835 0.86 -0.3 2,317,752 - +0.5 2,317,752 - -1.3 2,317,795 + +0.2 2,317,796 - +1.8 2,317,797 + -0.9 2,317,797 + -0.3 2,317,798 - +0.4 2,317,981 + PS417_10840 0.25 +0.2 2,317,981 + PS417_10840 0.25 -0.1 2,318,209 + PS417_10840 0.54 -1.5 2,318,494 + -0.3
Or see this region's nucleotide sequence