Experiment: Neomycin 0.04 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_05405 and PS417_05410 are separated by 96 nucleotides PS417_05410 and PS417_05415 are separated by 324 nucleotides
PS417_05405: PS417_05405 - L-alanine and D-alanine permease (from data) , at 1,211,485 to 1,212,903
_05405
PS417_05410: PS417_05410 - membrane protein, at 1,213,000 to 1,214,148
_05410
PS417_05415: PS417_05415 - glutamate synthase, at 1,214,473 to 1,216,092
_05415
Position (kb)
1212
1213
1214
1215 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1212.211 kb on - strand, within PS417_05405 at 1212.256 kb on + strand, within PS417_05405 at 1212.257 kb on - strand, within PS417_05405 at 1212.445 kb on + strand, within PS417_05405 at 1212.805 kb on + strand at 1212.805 kb on + strand at 1212.806 kb on - strand at 1212.806 kb on - strand at 1212.806 kb on - strand at 1212.806 kb on - strand at 1212.847 kb on + strand at 1212.847 kb on + strand at 1212.848 kb on - strand at 1213.025 kb on - strand at 1213.051 kb on - strand at 1213.051 kb on - strand at 1213.292 kb on + strand, within PS417_05410 at 1213.353 kb on - strand, within PS417_05410 at 1213.628 kb on + strand at 1213.628 kb on + strand, within PS417_05410 at 1213.891 kb on - strand, within PS417_05410 at 1213.891 kb on - strand, within PS417_05410 at 1213.902 kb on - strand, within PS417_05410 at 1214.165 kb on - strand at 1214.175 kb on + strand at 1214.207 kb on - strand at 1214.250 kb on - strand at 1214.250 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Neomycin 0.04 mg/ml remove 1,212,211 - PS417_05405 0.51 +1.6 1,212,256 + PS417_05405 0.54 +0.1 1,212,257 - PS417_05405 0.54 -0.3 1,212,445 + PS417_05405 0.68 +0.8 1,212,805 + -0.4 1,212,805 + +0.9 1,212,806 - +1.0 1,212,806 - -3.7 1,212,806 - +2.2 1,212,806 - +1.3 1,212,847 + -0.3 1,212,847 + +0.4 1,212,848 - +0.1 1,213,025 - +1.8 1,213,051 - +0.2 1,213,051 - -0.3 1,213,292 + PS417_05410 0.25 +1.5 1,213,353 - PS417_05410 0.31 +0.6 1,213,628 + -0.8 1,213,628 + PS417_05410 0.55 +0.5 1,213,891 - PS417_05410 0.78 +1.0 1,213,891 - PS417_05410 0.78 +0.8 1,213,902 - PS417_05410 0.79 +2.1 1,214,165 - -0.4 1,214,175 + +0.1 1,214,207 - -0.6 1,214,250 - -0.8 1,214,250 - -0.6
Or see this region's nucleotide sequence