Strain Fitness in Escherichia coli BW25113 around b2926

Experiment: a-Ketoglutaric (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntfbaA and pgk are separated by 214 nucleotidespgk and epd are separated by 49 nucleotides b2925: fbaA - fructose-bisphosphate aldolase (NCBI), at 3,068,187 to 3,069,266 fbaA b2926: pgk - phosphoglycerate kinase (NCBI), at 3,069,481 to 3,070,644 pgk b2927: epd - D-erythrose 4-phosphate dehydrogenase (NCBI), at 3,070,694 to 3,071,713 epd Position (kb) 3069 3070 3071Strain fitness (log2 ratio) -2 -1 0 1at 3069.297 kb on - strandat 3069.440 kb on - strandat 3069.440 kb on - strandat 3070.816 kb on - strand, within epdat 3071.084 kb on - strandat 3071.184 kb on + strand, within epdat 3071.199 kb on - strand, within epdat 3071.204 kb on - strand, within epdat 3071.204 kb on - strand, within epdat 3071.230 kb on + strand, within epdat 3071.230 kb on + strand, within epdat 3071.268 kb on + strand, within epdat 3071.294 kb on + strandat 3071.294 kb on + strand, within epdat 3071.309 kb on + strand, within epdat 3071.450 kb on + strand, within epdat 3071.464 kb on + strand, within epdat 3071.494 kb on + strand, within epdat 3071.536 kb on + strand, within epdat 3071.557 kb on - strand, within epdat 3071.557 kb on - strand, within epdat 3071.600 kb on - strand, within epd

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Per-strain Table

Position Strand Gene LocusTag Fraction a-Ketoglutaric (C)
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3,069,297 - +0.9
3,069,440 - +0.0
3,069,440 - +0.2
3,070,816 - epd b2927 0.12 -0.5
3,071,084 - +0.9
3,071,184 + epd b2927 0.48 -0.3
3,071,199 - epd b2927 0.50 -0.7
3,071,204 - epd b2927 0.50 +0.4
3,071,204 - epd b2927 0.50 -0.6
3,071,230 + epd b2927 0.53 -1.7
3,071,230 + epd b2927 0.53 -1.4
3,071,268 + epd b2927 0.56 -0.5
3,071,294 + +0.3
3,071,294 + epd b2927 0.59 -0.2
3,071,309 + epd b2927 0.60 -0.3
3,071,450 + epd b2927 0.74 -1.5
3,071,464 + epd b2927 0.75 -1.9
3,071,494 + epd b2927 0.78 -0.3
3,071,536 + epd b2927 0.83 +0.1
3,071,557 - epd b2927 0.85 -1.5
3,071,557 - epd b2927 0.85 -1.1
3,071,600 - epd b2927 0.89 +0.2

Or see this region's nucleotide sequence