Strain Fitness in Escherichia coli BW25113 around b2462

Experiment: a-Ketoglutaric (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nteutQ and eutP overlap by 26 nucleotideseutP and b2462 are separated by 12 nucleotidesb2462 and maeB are separated by 292 nucleotides b2460: eutQ - hypothetical protein (NCBI), at 2,572,324 to 2,573,025 eutQ b2461: eutP - conserved protein with nucleoside triphosphate hydrolase domain (NCBI), at 2,573,000 to 2,573,479 eutP b2462: b2462 - orf, hypothetical protein (VIMSS), at 2,573,492 to 2,573,827 b2462 b2463: maeB - malic enzyme (NCBI), at 2,574,120 to 2,576,399 maeB Position (kb) 2573 2574Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 2572.539 kb on + strand, within eutQat 2572.539 kb on + strand, within eutQat 2572.539 kb on + strand, within eutQat 2572.539 kb on + strand, within eutQat 2572.584 kb on - strand, within eutQat 2573.126 kb on + strand, within eutPat 2573.186 kb on - strand, within eutPat 2573.186 kb on - strand, within eutPat 2573.222 kb on - strand, within eutPat 2573.225 kb on + strand, within eutPat 2573.225 kb on + strand, within eutPat 2573.237 kb on - strand, within eutPat 2573.260 kb on + strand, within eutPat 2573.260 kb on + strand, within eutPat 2573.278 kb on + strand, within eutPat 2573.278 kb on + strand, within eutPat 2573.279 kb on - strand, within eutPat 2573.281 kb on + strand, within eutPat 2573.281 kb on + strand, within eutPat 2573.281 kb on + strand, within eutPat 2573.281 kb on + strand, within eutPat 2573.281 kb on + strand, within eutPat 2573.281 kb on - strand, within eutPat 2573.281 kb on - strand, within eutPat 2573.282 kb on + strand, within eutPat 2573.282 kb on + strand, within eutPat 2573.283 kb on + strand, within eutPat 2573.283 kb on + strand, within eutPat 2573.283 kb on + strand, within eutPat 2573.284 kb on - strand, within eutPat 2573.286 kb on - strand, within eutPat 2573.291 kb on - strand, within eutPat 2573.291 kb on - strand, within eutPat 2573.304 kb on + strand, within eutPat 2573.304 kb on + strand, within eutPat 2573.304 kb on + strand, within eutPat 2573.312 kb on - strand, within eutPat 2573.315 kb on + strand, within eutPat 2573.355 kb on - strand, within eutPat 2573.356 kb on - strand, within eutPat 2573.399 kb on - strand, within eutPat 2573.399 kb on - strand, within eutPat 2573.459 kb on + strandat 2573.459 kb on - strandat 2573.459 kb on - strandat 2573.459 kb on - strandat 2573.496 kb on - strandat 2573.496 kb on - strandat 2573.496 kb on - strandat 2573.497 kb on - strandat 2573.550 kb on + strand, within b2462at 2573.560 kb on - strand, within b2462at 2573.562 kb on - strand, within b2462at 2573.562 kb on - strand, within b2462at 2573.562 kb on - strand, within b2462at 2573.572 kb on - strand, within b2462at 2573.572 kb on - strand, within b2462at 2573.581 kb on - strand, within b2462at 2573.581 kb on - strand, within b2462at 2573.604 kb on - strand, within b2462at 2573.606 kb on + strand, within b2462at 2573.751 kb on - strand, within b2462at 2573.774 kb on - strand, within b2462at 2573.774 kb on - strand, within b2462at 2573.775 kb on + strand, within b2462at 2573.783 kb on - strand, within b2462at 2573.784 kb on - strand, within b2462at 2573.792 kb on - strand, within b2462at 2573.818 kb on + strandat 2573.830 kb on + strandat 2573.830 kb on + strandat 2573.838 kb on - strandat 2573.838 kb on - strandat 2573.886 kb on + strandat 2573.928 kb on - strandat 2573.933 kb on - strandat 2573.963 kb on + strandat 2573.963 kb on + strandat 2574.083 kb on - strandat 2574.103 kb on - strandat 2574.133 kb on - strandat 2574.145 kb on + strandat 2574.148 kb on - strandat 2574.152 kb on - strandat 2574.158 kb on + strandat 2574.158 kb on + strandat 2574.170 kb on + strandat 2574.173 kb on - strandat 2574.187 kb on - strandat 2574.187 kb on - strandat 2574.190 kb on - strandat 2574.202 kb on - strandat 2574.216 kb on + strandat 2574.225 kb on + strandat 2574.233 kb on - strandat 2574.378 kb on - strand, within maeBat 2574.380 kb on + strand, within maeBat 2574.575 kb on - strand, within maeBat 2574.575 kb on - strand, within maeBat 2574.648 kb on + strand, within maeBat 2574.648 kb on + strand, within maeBat 2574.711 kb on + strand, within maeB

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Per-strain Table

Position Strand Gene LocusTag Fraction a-Ketoglutaric (C)
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2,572,539 + eutQ b2460 0.31 +0.5
2,572,539 + eutQ b2460 0.31 +1.1
2,572,539 + eutQ b2460 0.31 +0.1
2,572,539 + eutQ b2460 0.31 -0.7
2,572,584 - eutQ b2460 0.37 -0.0
2,573,126 + eutP b2461 0.26 -0.1
2,573,186 - eutP b2461 0.39 +0.6
2,573,186 - eutP b2461 0.39 -0.6
2,573,222 - eutP b2461 0.46 -0.0
2,573,225 + eutP b2461 0.47 -0.8
2,573,225 + eutP b2461 0.47 -0.6
2,573,237 - eutP b2461 0.49 -0.2
2,573,260 + eutP b2461 0.54 -1.9
2,573,260 + eutP b2461 0.54 -0.6
2,573,278 + eutP b2461 0.58 +0.8
2,573,278 + eutP b2461 0.58 +0.6
2,573,279 - eutP b2461 0.58 +0.3
2,573,281 + eutP b2461 0.59 +1.8
2,573,281 + eutP b2461 0.59 -1.2
2,573,281 + eutP b2461 0.59 +0.4
2,573,281 + eutP b2461 0.59 -0.6
2,573,281 + eutP b2461 0.59 +0.0
2,573,281 - eutP b2461 0.59 +0.6
2,573,281 - eutP b2461 0.59 +0.2
2,573,282 + eutP b2461 0.59 -0.1
2,573,282 + eutP b2461 0.59 -0.1
2,573,283 + eutP b2461 0.59 -0.8
2,573,283 + eutP b2461 0.59 +0.7
2,573,283 + eutP b2461 0.59 -0.4
2,573,284 - eutP b2461 0.59 -0.6
2,573,286 - eutP b2461 0.60 +0.1
2,573,291 - eutP b2461 0.61 +0.7
2,573,291 - eutP b2461 0.61 -0.1
2,573,304 + eutP b2461 0.63 -1.0
2,573,304 + eutP b2461 0.63 -0.4
2,573,304 + eutP b2461 0.63 -0.2
2,573,312 - eutP b2461 0.65 +0.4
2,573,315 + eutP b2461 0.66 +0.2
2,573,355 - eutP b2461 0.74 +0.1
2,573,356 - eutP b2461 0.74 -0.1
2,573,399 - eutP b2461 0.83 +0.1
2,573,399 - eutP b2461 0.83 +0.6
2,573,459 + +0.6
2,573,459 - +0.8
2,573,459 - -0.9
2,573,459 - -0.4
2,573,496 - -0.5
2,573,496 - -1.3
2,573,496 - +0.1
2,573,497 - +0.3
2,573,550 + b2462 b2462 0.17 -0.0
2,573,560 - b2462 b2462 0.20 +1.3
2,573,562 - b2462 b2462 0.21 +0.4
2,573,562 - b2462 b2462 0.21 +0.7
2,573,562 - b2462 b2462 0.21 +0.8
2,573,572 - b2462 b2462 0.24 -1.1
2,573,572 - b2462 b2462 0.24 -0.7
2,573,581 - b2462 b2462 0.26 -1.9
2,573,581 - b2462 b2462 0.26 -0.3
2,573,604 - b2462 b2462 0.33 -1.4
2,573,606 + b2462 b2462 0.34 +0.4
2,573,751 - b2462 b2462 0.77 -0.1
2,573,774 - b2462 b2462 0.84 -0.0
2,573,774 - b2462 b2462 0.84 -0.6
2,573,775 + b2462 b2462 0.84 +0.5
2,573,783 - b2462 b2462 0.87 +0.2
2,573,784 - b2462 b2462 0.87 -1.6
2,573,792 - b2462 b2462 0.89 -0.0
2,573,818 + -0.1
2,573,830 + -0.0
2,573,830 + +0.2
2,573,838 - -0.3
2,573,838 - -1.2
2,573,886 + -1.4
2,573,928 - +0.6
2,573,933 - -0.8
2,573,963 + -0.6
2,573,963 + +1.6
2,574,083 - -1.4
2,574,103 - -0.0
2,574,133 - +0.6
2,574,145 + -1.9
2,574,148 - -0.9
2,574,152 - -3.1
2,574,158 + -1.6
2,574,158 + -1.8
2,574,170 + -2.4
2,574,173 - -2.9
2,574,187 - -2.3
2,574,187 - -2.4
2,574,190 - -1.2
2,574,202 - -2.2
2,574,216 + -1.0
2,574,225 + -1.2
2,574,233 - -0.9
2,574,378 - maeB b2463 0.11 -2.3
2,574,380 + maeB b2463 0.11 -1.3
2,574,575 - maeB b2463 0.20 -2.6
2,574,575 - maeB b2463 0.20 -1.3
2,574,648 + maeB b2463 0.23 -1.2
2,574,648 + maeB b2463 0.23 -1.1
2,574,711 + maeB b2463 0.26 -0.8

Or see this region's nucleotide sequence