Strain Fitness in Escherichia coli BW25113 around b2460

Experiment: a-Ketoglutaric (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nteutI and eutT overlap by 4 nucleotideseutT and eutQ overlap by 4 nucleotideseutQ and eutP overlap by 26 nucleotideseutP and b2462 are separated by 12 nucleotides b2458: eutI - predicted phosphotransacetylase subunit (NCBI), at 2,570,511 to 2,571,527 eutI b2459: eutT - predicted cobalamin adenosyltransferase in ethanolamine utilization (NCBI), at 2,571,524 to 2,572,327 eutT b2460: eutQ - hypothetical protein (NCBI), at 2,572,324 to 2,573,025 eutQ b2461: eutP - conserved protein with nucleoside triphosphate hydrolase domain (NCBI), at 2,573,000 to 2,573,479 eutP b2462: b2462 - orf, hypothetical protein (VIMSS), at 2,573,492 to 2,573,827 b2462 Position (kb) 2572 2573 2574Strain fitness (log2 ratio) -2 -1 0 1 2at 2571.330 kb on - strand, within eutIat 2571.337 kb on - strand, within eutIat 2571.337 kb on - strand, within eutIat 2571.441 kb on + strandat 2571.561 kb on + strandat 2571.561 kb on + strandat 2571.564 kb on - strandat 2571.599 kb on + strandat 2571.603 kb on - strandat 2571.603 kb on - strandat 2571.655 kb on + strand, within eutTat 2571.812 kb on + strand, within eutTat 2571.924 kb on + strand, within eutTat 2571.924 kb on + strand, within eutTat 2571.985 kb on - strand, within eutTat 2571.985 kb on - strand, within eutTat 2572.113 kb on + strand, within eutTat 2572.134 kb on + strand, within eutTat 2572.134 kb on + strand, within eutTat 2572.153 kb on - strand, within eutTat 2572.405 kb on + strand, within eutQat 2572.471 kb on - strand, within eutQat 2572.471 kb on - strand, within eutQat 2572.477 kb on + strand, within eutQat 2572.539 kb on + strand, within eutQat 2572.539 kb on + strand, within eutQat 2572.539 kb on + strand, within eutQat 2572.539 kb on + strand, within eutQat 2572.584 kb on - strand, within eutQat 2573.126 kb on + strand, within eutPat 2573.186 kb on - strand, within eutPat 2573.186 kb on - strand, within eutPat 2573.222 kb on - strand, within eutPat 2573.225 kb on + strand, within eutPat 2573.225 kb on + strand, within eutPat 2573.237 kb on - strand, within eutPat 2573.260 kb on + strand, within eutPat 2573.260 kb on + strand, within eutPat 2573.278 kb on + strand, within eutPat 2573.278 kb on + strand, within eutPat 2573.279 kb on - strand, within eutPat 2573.281 kb on + strand, within eutPat 2573.281 kb on + strand, within eutPat 2573.281 kb on + strand, within eutPat 2573.281 kb on + strand, within eutPat 2573.281 kb on + strand, within eutPat 2573.281 kb on - strand, within eutPat 2573.281 kb on - strand, within eutPat 2573.282 kb on + strand, within eutPat 2573.282 kb on + strand, within eutPat 2573.283 kb on + strand, within eutPat 2573.283 kb on + strand, within eutPat 2573.283 kb on + strand, within eutPat 2573.284 kb on - strand, within eutPat 2573.286 kb on - strand, within eutPat 2573.291 kb on - strand, within eutPat 2573.291 kb on - strand, within eutPat 2573.304 kb on + strand, within eutPat 2573.304 kb on + strand, within eutPat 2573.304 kb on + strand, within eutPat 2573.312 kb on - strand, within eutPat 2573.315 kb on + strand, within eutPat 2573.355 kb on - strand, within eutPat 2573.356 kb on - strand, within eutPat 2573.399 kb on - strand, within eutPat 2573.399 kb on - strand, within eutPat 2573.459 kb on + strandat 2573.459 kb on - strandat 2573.459 kb on - strandat 2573.459 kb on - strandat 2573.496 kb on - strandat 2573.496 kb on - strandat 2573.496 kb on - strandat 2573.497 kb on - strandat 2573.550 kb on + strand, within b2462at 2573.560 kb on - strand, within b2462at 2573.562 kb on - strand, within b2462at 2573.562 kb on - strand, within b2462at 2573.562 kb on - strand, within b2462at 2573.572 kb on - strand, within b2462at 2573.572 kb on - strand, within b2462at 2573.581 kb on - strand, within b2462at 2573.581 kb on - strand, within b2462at 2573.604 kb on - strand, within b2462at 2573.606 kb on + strand, within b2462at 2573.751 kb on - strand, within b2462at 2573.774 kb on - strand, within b2462at 2573.774 kb on - strand, within b2462at 2573.775 kb on + strand, within b2462at 2573.783 kb on - strand, within b2462at 2573.784 kb on - strand, within b2462at 2573.792 kb on - strand, within b2462at 2573.818 kb on + strandat 2573.830 kb on + strandat 2573.830 kb on + strandat 2573.838 kb on - strandat 2573.838 kb on - strandat 2573.886 kb on + strandat 2573.928 kb on - strandat 2573.933 kb on - strandat 2573.963 kb on + strandat 2573.963 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction a-Ketoglutaric (C)
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2,571,330 - eutI b2458 0.81 -0.3
2,571,337 - eutI b2458 0.81 -0.7
2,571,337 - eutI b2458 0.81 +0.6
2,571,441 + +1.5
2,571,561 + -0.6
2,571,561 + -0.6
2,571,564 - +0.2
2,571,599 + -0.7
2,571,603 - -0.1
2,571,603 - +0.9
2,571,655 + eutT b2459 0.16 -1.8
2,571,812 + eutT b2459 0.36 +0.4
2,571,924 + eutT b2459 0.50 -0.1
2,571,924 + eutT b2459 0.50 +0.3
2,571,985 - eutT b2459 0.57 +0.2
2,571,985 - eutT b2459 0.57 +2.0
2,572,113 + eutT b2459 0.73 -0.6
2,572,134 + eutT b2459 0.76 -1.0
2,572,134 + eutT b2459 0.76 -0.6
2,572,153 - eutT b2459 0.78 -0.3
2,572,405 + eutQ b2460 0.12 +0.5
2,572,471 - eutQ b2460 0.21 -1.3
2,572,471 - eutQ b2460 0.21 -1.3
2,572,477 + eutQ b2460 0.22 +0.7
2,572,539 + eutQ b2460 0.31 +0.5
2,572,539 + eutQ b2460 0.31 +1.1
2,572,539 + eutQ b2460 0.31 +0.1
2,572,539 + eutQ b2460 0.31 -0.7
2,572,584 - eutQ b2460 0.37 -0.0
2,573,126 + eutP b2461 0.26 -0.1
2,573,186 - eutP b2461 0.39 +0.6
2,573,186 - eutP b2461 0.39 -0.6
2,573,222 - eutP b2461 0.46 -0.0
2,573,225 + eutP b2461 0.47 -0.8
2,573,225 + eutP b2461 0.47 -0.6
2,573,237 - eutP b2461 0.49 -0.2
2,573,260 + eutP b2461 0.54 -1.9
2,573,260 + eutP b2461 0.54 -0.6
2,573,278 + eutP b2461 0.58 +0.8
2,573,278 + eutP b2461 0.58 +0.6
2,573,279 - eutP b2461 0.58 +0.3
2,573,281 + eutP b2461 0.59 +1.8
2,573,281 + eutP b2461 0.59 -1.2
2,573,281 + eutP b2461 0.59 +0.4
2,573,281 + eutP b2461 0.59 -0.6
2,573,281 + eutP b2461 0.59 +0.0
2,573,281 - eutP b2461 0.59 +0.6
2,573,281 - eutP b2461 0.59 +0.2
2,573,282 + eutP b2461 0.59 -0.1
2,573,282 + eutP b2461 0.59 -0.1
2,573,283 + eutP b2461 0.59 -0.8
2,573,283 + eutP b2461 0.59 +0.7
2,573,283 + eutP b2461 0.59 -0.4
2,573,284 - eutP b2461 0.59 -0.6
2,573,286 - eutP b2461 0.60 +0.1
2,573,291 - eutP b2461 0.61 +0.7
2,573,291 - eutP b2461 0.61 -0.1
2,573,304 + eutP b2461 0.63 -1.0
2,573,304 + eutP b2461 0.63 -0.4
2,573,304 + eutP b2461 0.63 -0.2
2,573,312 - eutP b2461 0.65 +0.4
2,573,315 + eutP b2461 0.66 +0.2
2,573,355 - eutP b2461 0.74 +0.1
2,573,356 - eutP b2461 0.74 -0.1
2,573,399 - eutP b2461 0.83 +0.1
2,573,399 - eutP b2461 0.83 +0.6
2,573,459 + +0.6
2,573,459 - +0.8
2,573,459 - -0.9
2,573,459 - -0.4
2,573,496 - -0.5
2,573,496 - -1.3
2,573,496 - +0.1
2,573,497 - +0.3
2,573,550 + b2462 b2462 0.17 -0.0
2,573,560 - b2462 b2462 0.20 +1.3
2,573,562 - b2462 b2462 0.21 +0.4
2,573,562 - b2462 b2462 0.21 +0.7
2,573,562 - b2462 b2462 0.21 +0.8
2,573,572 - b2462 b2462 0.24 -1.1
2,573,572 - b2462 b2462 0.24 -0.7
2,573,581 - b2462 b2462 0.26 -1.9
2,573,581 - b2462 b2462 0.26 -0.3
2,573,604 - b2462 b2462 0.33 -1.4
2,573,606 + b2462 b2462 0.34 +0.4
2,573,751 - b2462 b2462 0.77 -0.1
2,573,774 - b2462 b2462 0.84 -0.0
2,573,774 - b2462 b2462 0.84 -0.6
2,573,775 + b2462 b2462 0.84 +0.5
2,573,783 - b2462 b2462 0.87 +0.2
2,573,784 - b2462 b2462 0.87 -1.6
2,573,792 - b2462 b2462 0.89 -0.0
2,573,818 + -0.1
2,573,830 + -0.0
2,573,830 + +0.2
2,573,838 - -0.3
2,573,838 - -1.2
2,573,886 + -1.4
2,573,928 - +0.6
2,573,933 - -0.8
2,573,963 + -0.6
2,573,963 + +1.6

Or see this region's nucleotide sequence