Strain Fitness in Escherichia coli BW25113 around b0430

Experiment: a-Ketoglutaric (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntcyoE and cyoD are separated by 11 nucleotidescyoD and cyoC overlap by 1 nucleotidescyoC and cyoB overlap by 11 nucleotides b0428: cyoE - protoheme IX farnesyltransferase (NCBI), at 446,039 to 446,929 cyoE b0429: cyoD - cytochrome o ubiquinol oxidase subunit IV (NCBI), at 446,941 to 447,270 cyoD b0430: cyoC - cytochrome o ubiquinol oxidase subunit III (NCBI), at 447,270 to 447,884 cyoC b0431: cyoB - cytochrome o ubiquinol oxidase subunit I (NCBI), at 447,874 to 449,865 cyoB Position (kb) 447 448Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 446.297 kb on - strand, within cyoEat 446.394 kb on + strand, within cyoEat 446.394 kb on + strand, within cyoEat 446.394 kb on + strand, within cyoEat 446.407 kb on - strand, within cyoEat 446.525 kb on + strand, within cyoEat 446.525 kb on + strand, within cyoEat 446.533 kb on - strand, within cyoEat 446.562 kb on - strand, within cyoEat 446.562 kb on - strand, within cyoEat 446.657 kb on + strand, within cyoEat 446.657 kb on + strand, within cyoEat 446.673 kb on - strand, within cyoEat 446.677 kb on + strand, within cyoEat 446.677 kb on + strand, within cyoEat 446.796 kb on - strand, within cyoEat 446.824 kb on + strand, within cyoEat 446.832 kb on - strand, within cyoEat 446.880 kb on + strandat 446.880 kb on + strandat 446.932 kb on + strandat 446.944 kb on + strandat 446.981 kb on - strand, within cyoDat 447.018 kb on - strand, within cyoDat 447.093 kb on - strand, within cyoDat 447.093 kb on - strand, within cyoDat 447.105 kb on - strand, within cyoDat 447.161 kb on - strand, within cyoDat 447.167 kb on + strand, within cyoDat 447.175 kb on - strand, within cyoDat 447.201 kb on + strand, within cyoDat 447.223 kb on - strand, within cyoDat 447.285 kb on - strandat 447.303 kb on - strandat 447.347 kb on + strand, within cyoCat 447.351 kb on + strand, within cyoCat 447.373 kb on - strand, within cyoCat 447.407 kb on + strand, within cyoCat 447.418 kb on - strand, within cyoCat 447.445 kb on - strand, within cyoCat 447.492 kb on + strand, within cyoCat 447.492 kb on + strand, within cyoCat 447.521 kb on + strand, within cyoCat 447.570 kb on + strand, within cyoCat 447.580 kb on + strand, within cyoCat 447.679 kb on + strand, within cyoCat 447.956 kb on + strandat 447.964 kb on - strandat 447.964 kb on - strandat 447.989 kb on + strandat 448.002 kb on - strandat 448.005 kb on + strandat 448.092 kb on - strand, within cyoBat 448.092 kb on - strand, within cyoBat 448.134 kb on - strand, within cyoBat 448.134 kb on - strand, within cyoBat 448.238 kb on - strand, within cyoBat 448.364 kb on - strand, within cyoBat 448.364 kb on - strand, within cyoBat 448.388 kb on + strand, within cyoBat 448.388 kb on + strand, within cyoBat 448.388 kb on + strand, within cyoBat 448.391 kb on - strand, within cyoBat 448.391 kb on - strand, within cyoBat 448.396 kb on - strand, within cyoBat 448.396 kb on - strand, within cyoBat 448.396 kb on - strand, within cyoBat 448.434 kb on - strand, within cyoBat 448.443 kb on - strand, within cyoBat 448.443 kb on - strand, within cyoBat 448.455 kb on - strand, within cyoBat 448.457 kb on + strand, within cyoBat 448.466 kb on + strand, within cyoBat 448.549 kb on + strand, within cyoBat 448.557 kb on - strand, within cyoBat 448.641 kb on - strand, within cyoBat 448.678 kb on + strand, within cyoBat 448.722 kb on + strand, within cyoBat 448.722 kb on - strand, within cyoBat 448.730 kb on - strand, within cyoBat 448.750 kb on - strand, within cyoBat 448.844 kb on + strand, within cyoBat 448.844 kb on + strand, within cyoBat 448.846 kb on + strand, within cyoBat 448.854 kb on + strand, within cyoBat 448.854 kb on + strand, within cyoBat 448.854 kb on - strand, within cyoBat 448.880 kb on + strand, within cyoB

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Per-strain Table

Position Strand Gene LocusTag Fraction a-Ketoglutaric (C)
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446,297 - cyoE b0428 0.29 -0.8
446,394 + cyoE b0428 0.40 -2.2
446,394 + cyoE b0428 0.40 -2.7
446,394 + cyoE b0428 0.40 -0.5
446,407 - cyoE b0428 0.41 -1.9
446,525 + cyoE b0428 0.55 -0.8
446,525 + cyoE b0428 0.55 -0.0
446,533 - cyoE b0428 0.55 -1.0
446,562 - cyoE b0428 0.59 -2.3
446,562 - cyoE b0428 0.59 -1.0
446,657 + cyoE b0428 0.69 -1.6
446,657 + cyoE b0428 0.69 -1.5
446,673 - cyoE b0428 0.71 -2.3
446,677 + cyoE b0428 0.72 +1.8
446,677 + cyoE b0428 0.72 -0.5
446,796 - cyoE b0428 0.85 -1.1
446,824 + cyoE b0428 0.88 -1.5
446,832 - cyoE b0428 0.89 -1.1
446,880 + -2.1
446,880 + -3.1
446,932 + -0.6
446,944 + -0.2
446,981 - cyoD b0429 0.12 -0.7
447,018 - cyoD b0429 0.23 -1.6
447,093 - cyoD b0429 0.46 -1.0
447,093 - cyoD b0429 0.46 -0.7
447,105 - cyoD b0429 0.50 -2.4
447,161 - cyoD b0429 0.67 -1.2
447,167 + cyoD b0429 0.68 -1.5
447,175 - cyoD b0429 0.71 -1.1
447,201 + cyoD b0429 0.79 -1.7
447,223 - cyoD b0429 0.85 -1.6
447,285 - -2.4
447,303 - -1.4
447,347 + cyoC b0430 0.13 -1.9
447,351 + cyoC b0430 0.13 -3.3
447,373 - cyoC b0430 0.17 -2.7
447,407 + cyoC b0430 0.22 -1.9
447,418 - cyoC b0430 0.24 -1.4
447,445 - cyoC b0430 0.28 -1.3
447,492 + cyoC b0430 0.36 -1.9
447,492 + cyoC b0430 0.36 -1.4
447,521 + cyoC b0430 0.41 -0.5
447,570 + cyoC b0430 0.49 -1.8
447,580 + cyoC b0430 0.50 -1.9
447,679 + cyoC b0430 0.67 -1.4
447,956 + -1.9
447,964 - -1.4
447,964 - -2.0
447,989 + -0.1
448,002 - -1.2
448,005 + -2.5
448,092 - cyoB b0431 0.11 -3.2
448,092 - cyoB b0431 0.11 -0.2
448,134 - cyoB b0431 0.13 -0.8
448,134 - cyoB b0431 0.13 -0.7
448,238 - cyoB b0431 0.18 -0.9
448,364 - cyoB b0431 0.25 -3.2
448,364 - cyoB b0431 0.25 -1.5
448,388 + cyoB b0431 0.26 -0.5
448,388 + cyoB b0431 0.26 -1.5
448,388 + cyoB b0431 0.26 -2.8
448,391 - cyoB b0431 0.26 -0.9
448,391 - cyoB b0431 0.26 -1.2
448,396 - cyoB b0431 0.26 -2.7
448,396 - cyoB b0431 0.26 +0.2
448,396 - cyoB b0431 0.26 +0.5
448,434 - cyoB b0431 0.28 -3.1
448,443 - cyoB b0431 0.29 -1.0
448,443 - cyoB b0431 0.29 -0.2
448,455 - cyoB b0431 0.29 -1.9
448,457 + cyoB b0431 0.29 -2.8
448,466 + cyoB b0431 0.30 -2.0
448,549 + cyoB b0431 0.34 -1.7
448,557 - cyoB b0431 0.34 -0.8
448,641 - cyoB b0431 0.39 -1.1
448,678 + cyoB b0431 0.40 -1.0
448,722 + cyoB b0431 0.43 -3.6
448,722 - cyoB b0431 0.43 -1.5
448,730 - cyoB b0431 0.43 -2.6
448,750 - cyoB b0431 0.44 -1.5
448,844 + cyoB b0431 0.49 -2.8
448,844 + cyoB b0431 0.49 -0.7
448,846 + cyoB b0431 0.49 -0.9
448,854 + cyoB b0431 0.49 -1.1
448,854 + cyoB b0431 0.49 -1.2
448,854 - cyoB b0431 0.49 -0.4
448,880 + cyoB b0431 0.51 -2.0

Or see this region's nucleotide sequence