Experiment: a-Ketoglutaric (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt aroL and yaiA are separated by 49 nucleotides yaiA and aroM are separated by 257 nucleotides aroM and yaiE are separated by 71 nucleotides yaiE and ykiA are separated by 147 nucleotides
b0388: aroL - shikimate kinase II (NCBI), at 405,629 to 406,153
aroL
b0389: yaiA - hypothetical protein (NCBI), at 406,203 to 406,394
yaiA
b0390: aroM - hypothetical protein (NCBI), at 406,652 to 407,329
aroM
b0391: yaiE - hypothetical protein (NCBI), at 407,401 to 407,685
yaiE
b0392: ykiA - hypothetical protein (NCBI), at 407,833 to 408,174
ykiA
Position (kb) 406
407
408 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3
4
5
6
7
8 at 405.675 kb on - strand at 405.867 kb on - strand, within aroL at 405.944 kb on + strand, within aroL at 405.944 kb on + strand, within aroL at 405.944 kb on + strand, within aroL at 405.952 kb on - strand, within aroL at 405.952 kb on - strand, within aroL at 406.049 kb on + strand, within aroL at 406.057 kb on - strand, within aroL at 406.095 kb on + strand, within aroL at 406.169 kb on + strand at 406.225 kb on - strand, within yaiA at 406.381 kb on + strand at 406.391 kb on - strand at 406.391 kb on - strand at 406.405 kb on - strand at 406.407 kb on + strand at 406.592 kb on + strand at 406.597 kb on + strand at 406.683 kb on + strand at 406.688 kb on + strand at 406.688 kb on + strand at 406.688 kb on + strand at 406.688 kb on + strand at 406.691 kb on - strand at 406.749 kb on + strand, within aroM at 406.757 kb on + strand, within aroM at 406.757 kb on + strand, within aroM at 406.776 kb on + strand, within aroM at 406.807 kb on + strand, within aroM at 406.819 kb on - strand, within aroM at 406.819 kb on - strand, within aroM at 406.913 kb on + strand, within aroM at 406.913 kb on + strand, within aroM at 406.913 kb on + strand, within aroM at 406.942 kb on + strand, within aroM at 406.942 kb on + strand, within aroM at 406.960 kb on + strand, within aroM at 406.960 kb on - strand, within aroM at 406.960 kb on - strand, within aroM at 406.994 kb on - strand, within aroM at 407.020 kb on + strand, within aroM at 407.052 kb on - strand, within aroM at 407.052 kb on - strand, within aroM at 407.131 kb on + strand, within aroM at 407.139 kb on - strand, within aroM at 407.139 kb on - strand, within aroM at 407.139 kb on - strand, within aroM at 407.139 kb on - strand, within aroM at 407.210 kb on - strand, within aroM at 407.268 kb on + strand at 407.357 kb on - strand at 407.404 kb on + strand at 407.404 kb on + strand at 407.457 kb on + strand, within yaiE at 407.462 kb on + strand, within yaiE at 407.462 kb on + strand, within yaiE at 407.477 kb on + strand, within yaiE at 407.483 kb on + strand, within yaiE at 407.549 kb on - strand, within yaiE at 407.549 kb on - strand, within yaiE at 407.562 kb on + strand, within yaiE at 407.590 kb on - strand, within yaiE at 407.590 kb on - strand, within yaiE at 407.590 kb on - strand, within yaiE at 407.592 kb on + strand, within yaiE at 407.610 kb on + strand, within yaiE at 407.648 kb on + strand, within yaiE at 407.649 kb on + strand, within yaiE at 407.650 kb on + strand, within yaiE at 407.660 kb on + strand at 407.660 kb on + strand at 407.660 kb on + strand at 407.660 kb on + strand at 407.660 kb on + strand at 407.660 kb on + strand at 407.660 kb on + strand at 407.660 kb on + strand at 407.660 kb on + strand at 407.660 kb on + strand at 407.662 kb on + strand at 407.662 kb on + strand at 407.668 kb on - strand at 407.668 kb on - strand at 407.668 kb on - strand at 407.670 kb on + strand at 407.678 kb on + strand at 407.682 kb on + strand at 407.686 kb on + strand at 407.686 kb on + strand at 407.787 kb on + strand at 407.787 kb on + strand at 407.802 kb on + strand at 407.811 kb on - strand at 407.828 kb on - strand at 407.828 kb on - strand at 407.828 kb on - strand at 407.853 kb on - strand at 407.853 kb on - strand at 407.858 kb on - strand at 407.862 kb on + strand at 407.878 kb on + strand, within ykiA at 407.878 kb on + strand, within ykiA at 407.884 kb on - strand, within ykiA at 407.884 kb on - strand, within ykiA at 407.888 kb on + strand, within ykiA at 407.888 kb on + strand, within ykiA at 407.893 kb on - strand, within ykiA at 407.893 kb on - strand, within ykiA at 407.893 kb on - strand, within ykiA at 407.934 kb on + strand, within ykiA at 407.942 kb on + strand, within ykiA at 407.942 kb on + strand, within ykiA at 407.942 kb on + strand, within ykiA at 407.942 kb on - strand, within ykiA at 407.942 kb on - strand, within ykiA at 407.943 kb on + strand, within ykiA at 407.943 kb on + strand, within ykiA at 407.948 kb on - strand, within ykiA at 407.948 kb on - strand, within ykiA at 407.956 kb on - strand, within ykiA at 407.956 kb on - strand, within ykiA at 407.958 kb on + strand, within ykiA at 407.958 kb on + strand, within ykiA at 407.958 kb on + strand, within ykiA at 407.963 kb on + strand, within ykiA at 407.966 kb on + strand, within ykiA at 407.966 kb on + strand, within ykiA at 407.966 kb on + strand, within ykiA at 407.966 kb on - strand, within ykiA at 407.967 kb on + strand, within ykiA at 407.968 kb on + strand, within ykiA at 407.969 kb on - strand, within ykiA at 407.973 kb on + strand, within ykiA at 407.975 kb on - strand, within ykiA at 407.976 kb on - strand, within ykiA at 407.977 kb on + strand, within ykiA at 407.977 kb on - strand, within ykiA at 407.977 kb on - strand, within ykiA at 407.978 kb on + strand, within ykiA at 407.980 kb on + strand, within ykiA at 407.980 kb on + strand, within ykiA at 407.987 kb on - strand, within ykiA at 407.998 kb on + strand, within ykiA at 407.998 kb on + strand, within ykiA at 407.999 kb on - strand, within ykiA at 407.999 kb on - strand, within ykiA at 408.005 kb on - strand, within ykiA at 408.012 kb on + strand, within ykiA at 408.041 kb on + strand, within ykiA at 408.046 kb on + strand, within ykiA at 408.046 kb on + strand, within ykiA at 408.049 kb on - strand, within ykiA at 408.050 kb on - strand, within ykiA at 408.113 kb on + strand, within ykiA at 408.167 kb on + strand at 408.188 kb on - strand at 408.193 kb on + strand at 408.193 kb on + strand at 408.194 kb on + strand at 408.195 kb on - strand at 408.195 kb on - strand at 408.200 kb on + strand at 408.200 kb on - strand at 408.200 kb on - strand at 408.200 kb on - strand at 408.200 kb on - strand at 408.250 kb on + strand at 408.255 kb on + strand at 408.268 kb on - strand at 408.279 kb on - strand at 408.279 kb on - strand at 408.282 kb on - strand at 408.283 kb on + strand at 408.291 kb on - strand at 408.291 kb on - strand at 408.292 kb on - strand at 408.296 kb on - strand at 408.296 kb on - strand at 408.301 kb on - strand at 408.301 kb on - strand at 408.301 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction a-Ketoglutaric (C) remove 405,675 - +0.3 405,867 - aroL b0388 0.45 +1.2 405,944 + aroL b0388 0.60 +0.5 405,944 + aroL b0388 0.60 +0.6 405,944 + aroL b0388 0.60 -0.1 405,952 - aroL b0388 0.62 +0.8 405,952 - aroL b0388 0.62 +1.4 406,049 + aroL b0388 0.80 +1.6 406,057 - aroL b0388 0.82 +0.4 406,095 + aroL b0388 0.89 -0.5 406,169 + +0.5 406,225 - yaiA b0389 0.11 -0.3 406,381 + +0.0 406,391 - -0.5 406,391 - -0.7 406,405 - +0.2 406,407 + +0.2 406,592 + +2.1 406,597 + +2.1 406,683 + -2.3 406,688 + -2.7 406,688 + -2.7 406,688 + -1.4 406,688 + -3.5 406,691 - -3.4 406,749 + aroM b0390 0.14 -2.1 406,757 + aroM b0390 0.15 -2.2 406,757 + aroM b0390 0.15 -2.5 406,776 + aroM b0390 0.18 -3.0 406,807 + aroM b0390 0.23 -2.4 406,819 - aroM b0390 0.25 -2.0 406,819 - aroM b0390 0.25 -1.2 406,913 + aroM b0390 0.38 -4.1 406,913 + aroM b0390 0.38 -3.1 406,913 + aroM b0390 0.38 -4.2 406,942 + aroM b0390 0.43 -2.7 406,942 + aroM b0390 0.43 -2.5 406,960 + aroM b0390 0.45 -3.3 406,960 - aroM b0390 0.45 -2.2 406,960 - aroM b0390 0.45 -1.8 406,994 - aroM b0390 0.50 -5.2 407,020 + aroM b0390 0.54 -2.1 407,052 - aroM b0390 0.59 -4.3 407,052 - aroM b0390 0.59 -3.5 407,131 + aroM b0390 0.71 -1.4 407,139 - aroM b0390 0.72 -1.7 407,139 - aroM b0390 0.72 -4.3 407,139 - aroM b0390 0.72 -1.5 407,139 - aroM b0390 0.72 -1.5 407,210 - aroM b0390 0.82 -1.5 407,268 + -3.7 407,357 - -0.8 407,404 + -1.7 407,404 + -1.2 407,457 + yaiE b0391 0.20 -0.7 407,462 + yaiE b0391 0.21 +0.7 407,462 + yaiE b0391 0.21 +0.2 407,477 + yaiE b0391 0.27 -0.1 407,483 + yaiE b0391 0.29 -0.8 407,549 - yaiE b0391 0.52 -0.1 407,549 - yaiE b0391 0.52 +0.1 407,562 + yaiE b0391 0.56 -0.0 407,590 - yaiE b0391 0.66 -0.7 407,590 - yaiE b0391 0.66 -1.5 407,590 - yaiE b0391 0.66 -0.8 407,592 + yaiE b0391 0.67 +0.6 407,610 + yaiE b0391 0.73 -1.2 407,648 + yaiE b0391 0.87 +0.4 407,649 + yaiE b0391 0.87 -1.2 407,650 + yaiE b0391 0.87 -0.1 407,660 + +0.8 407,660 + +0.5 407,660 + +1.1 407,660 + -0.1 407,660 + +0.5 407,660 + +1.3 407,660 + +0.9 407,660 + +0.4 407,660 + +0.3 407,660 + +7.8 407,662 + +0.4 407,662 + +0.8 407,668 - -0.1 407,668 - -2.4 407,668 - +0.7 407,670 + +0.6 407,678 + -0.1 407,682 + +0.3 407,686 + -1.9 407,686 + -1.1 407,787 + -0.3 407,787 + +0.2 407,802 + +0.8 407,811 - +1.6 407,828 - +0.4 407,828 - +0.1 407,828 - -0.4 407,853 - -0.8 407,853 - -2.0 407,858 - -0.2 407,862 + -1.1 407,878 + ykiA b0392 0.13 +0.4 407,878 + ykiA b0392 0.13 +0.9 407,884 - ykiA b0392 0.15 -0.3 407,884 - ykiA b0392 0.15 -0.0 407,888 + ykiA b0392 0.16 +0.4 407,888 + ykiA b0392 0.16 -1.1 407,893 - ykiA b0392 0.18 +0.2 407,893 - ykiA b0392 0.18 +0.7 407,893 - ykiA b0392 0.18 +0.1 407,934 + ykiA b0392 0.30 +0.6 407,942 + ykiA b0392 0.32 -0.1 407,942 + ykiA b0392 0.32 +3.8 407,942 + ykiA b0392 0.32 -0.5 407,942 - ykiA b0392 0.32 +0.1 407,942 - ykiA b0392 0.32 +0.2 407,943 + ykiA b0392 0.32 -0.0 407,943 + ykiA b0392 0.32 +0.3 407,948 - ykiA b0392 0.34 +5.0 407,948 - ykiA b0392 0.34 +5.2 407,956 - ykiA b0392 0.36 +1.3 407,956 - ykiA b0392 0.36 -0.8 407,958 + ykiA b0392 0.37 +0.2 407,958 + ykiA b0392 0.37 +0.5 407,958 + ykiA b0392 0.37 +0.8 407,963 + ykiA b0392 0.38 +0.0 407,966 + ykiA b0392 0.39 -0.1 407,966 + ykiA b0392 0.39 +0.4 407,966 + ykiA b0392 0.39 +0.6 407,966 - ykiA b0392 0.39 -0.3 407,967 + ykiA b0392 0.39 +0.6 407,968 + ykiA b0392 0.39 -1.5 407,969 - ykiA b0392 0.40 -0.1 407,973 + ykiA b0392 0.41 -0.6 407,975 - ykiA b0392 0.42 -0.4 407,976 - ykiA b0392 0.42 +0.6 407,977 + ykiA b0392 0.42 -1.5 407,977 - ykiA b0392 0.42 -0.7 407,977 - ykiA b0392 0.42 -1.8 407,978 + ykiA b0392 0.42 -0.2 407,980 + ykiA b0392 0.43 +0.3 407,980 + ykiA b0392 0.43 +0.2 407,987 - ykiA b0392 0.45 -0.4 407,998 + ykiA b0392 0.48 +0.5 407,998 + ykiA b0392 0.48 -0.0 407,999 - ykiA b0392 0.49 -0.8 407,999 - ykiA b0392 0.49 +0.1 408,005 - ykiA b0392 0.50 +1.3 408,012 + ykiA b0392 0.52 +0.3 408,041 + ykiA b0392 0.61 -1.7 408,046 + ykiA b0392 0.62 +0.2 408,046 + ykiA b0392 0.62 +0.6 408,049 - ykiA b0392 0.63 +0.3 408,050 - ykiA b0392 0.63 -0.1 408,113 + ykiA b0392 0.82 +0.6 408,167 + +0.5 408,188 - -0.1 408,193 + -0.5 408,193 + +0.9 408,194 + -1.2 408,195 - +0.0 408,195 - -0.0 408,200 + -1.3 408,200 - +0.3 408,200 - +1.2 408,200 - +0.4 408,200 - -0.0 408,250 + +0.6 408,255 + +0.2 408,268 - -0.3 408,279 - +1.8 408,279 - +0.9 408,282 - +0.4 408,283 + -0.1 408,291 - -1.8 408,291 - +0.5 408,292 - +0.8 408,296 - +0.0 408,296 - -0.5 408,301 - -0.1 408,301 - +0.2 408,301 - +0.6
Or see this region's nucleotide sequence