Experiment: a-Ketoglutaric (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt codB and codA overlap by 11 nucleotides codA and cynR are separated by 336 nucleotides
b0336: codB - cytosine transporter (NCBI), at 354,146 to 355,405
codB
b0337: codA - cytosine deaminase (NCBI), at 355,395 to 356,678
codA
b0338: cynR - DNA-binding transcriptional dual regulator (NCBI), at 357,015 to 357,914
cynR
Position (kb) 355
356
357 Strain fitness (log2 ratio) -2
-1
0
1
2 at 354.423 kb on - strand, within codB at 354.431 kb on - strand, within codB at 354.480 kb on + strand, within codB at 354.773 kb on - strand, within codB at 354.773 kb on - strand, within codB at 354.885 kb on + strand, within codB at 354.893 kb on - strand, within codB at 354.935 kb on - strand, within codB at 355.005 kb on - strand, within codB at 355.082 kb on - strand, within codB at 355.086 kb on + strand, within codB at 355.153 kb on - strand, within codB at 355.220 kb on - strand, within codB at 355.262 kb on - strand, within codB at 355.265 kb on + strand, within codB at 355.307 kb on + strand at 355.315 kb on - strand at 355.337 kb on - strand at 355.462 kb on - strand at 355.463 kb on - strand at 355.463 kb on - strand at 355.592 kb on - strand, within codA at 355.764 kb on - strand, within codA at 355.764 kb on - strand, within codA at 355.790 kb on - strand, within codA at 355.825 kb on - strand, within codA at 355.996 kb on - strand, within codA at 355.996 kb on - strand, within codA at 356.088 kb on + strand, within codA at 356.150 kb on + strand, within codA at 356.153 kb on + strand, within codA at 356.178 kb on - strand, within codA at 356.295 kb on - strand, within codA at 356.295 kb on - strand, within codA at 356.306 kb on - strand, within codA at 356.342 kb on - strand, within codA at 356.390 kb on + strand, within codA at 356.390 kb on + strand, within codA at 356.467 kb on + strand, within codA at 356.704 kb on - strand at 356.743 kb on - strand at 356.743 kb on - strand at 356.748 kb on - strand at 356.774 kb on - strand at 356.833 kb on + strand at 356.850 kb on + strand at 356.850 kb on + strand at 356.880 kb on + strand at 356.880 kb on + strand at 356.966 kb on + strand at 356.966 kb on + strand at 357.071 kb on - strand at 357.071 kb on - strand at 357.271 kb on - strand, within cynR at 357.274 kb on - strand, within cynR at 357.274 kb on - strand, within cynR at 357.386 kb on - strand, within cynR at 357.661 kb on - strand, within cynR
Per-strain Table
Position Strand Gene LocusTag Fraction a-Ketoglutaric (C) remove 354,423 - codB b0336 0.22 +0.1 354,431 - codB b0336 0.23 -0.5 354,480 + codB b0336 0.27 +0.6 354,773 - codB b0336 0.50 +0.2 354,773 - codB b0336 0.50 -0.2 354,885 + codB b0336 0.59 -2.0 354,893 - codB b0336 0.59 -0.4 354,935 - codB b0336 0.63 +0.4 355,005 - codB b0336 0.68 -1.1 355,082 - codB b0336 0.74 +0.6 355,086 + codB b0336 0.75 -1.9 355,153 - codB b0336 0.80 -0.6 355,220 - codB b0336 0.85 -1.4 355,262 - codB b0336 0.89 +0.6 355,265 + codB b0336 0.89 +0.6 355,307 + +0.9 355,315 - -0.2 355,337 - +1.0 355,462 - +0.4 355,463 - -0.3 355,463 - +0.8 355,592 - codA b0337 0.15 -1.8 355,764 - codA b0337 0.29 +0.9 355,764 - codA b0337 0.29 +0.1 355,790 - codA b0337 0.31 -1.8 355,825 - codA b0337 0.33 -2.4 355,996 - codA b0337 0.47 -0.8 355,996 - codA b0337 0.47 -2.3 356,088 + codA b0337 0.54 +0.2 356,150 + codA b0337 0.59 -0.7 356,153 + codA b0337 0.59 +0.6 356,178 - codA b0337 0.61 +0.9 356,295 - codA b0337 0.70 -0.8 356,295 - codA b0337 0.70 -0.4 356,306 - codA b0337 0.71 +0.0 356,342 - codA b0337 0.74 -1.0 356,390 + codA b0337 0.77 +0.9 356,390 + codA b0337 0.77 +0.2 356,467 + codA b0337 0.83 -1.0 356,704 - -0.2 356,743 - +1.1 356,743 - +0.7 356,748 - -1.1 356,774 - -0.0 356,833 + -0.2 356,850 + -1.6 356,850 + -1.9 356,880 + -0.3 356,880 + -0.6 356,966 + -1.2 356,966 + +0.4 357,071 - +0.3 357,071 - +1.8 357,271 - cynR b0338 0.28 -0.8 357,274 - cynR b0338 0.29 +0.5 357,274 - cynR b0338 0.29 -0.5 357,386 - cynR b0338 0.41 +0.2 357,661 - cynR b0338 0.72 +0.2
Or see this region's nucleotide sequence