Strain Fitness in Escherichia coli BW25113 around b0123

Experiment: a-Ketoglutaric (C)

Add experiment(s):

Zoom: Pan:

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

download SVG
500 ntspeE and yacC are separated by 105 nucleotidesyacC and cueO are separated by 165 nucleotidescueO and gcd are separated by 201 nucleotides b0121: speE - spermidine synthase (NCBI), at 135,598 to 136,464 speE b0122: yacC - orf, hypothetical protein (VIMSS), at 136,570 to 136,917 yacC b0123: cueO - multicopper oxidase (laccase) (NCBI), at 137,083 to 138,633 cueO b0124: gcd - glucose dehydrogenase (NCBI), at 138,835 to 141,225 gcd Position (kb) 137 138 139Strain fitness (log2 ratio) -2 -1 0 1 2 3 4at 136.088 kb on + strand, within speEat 136.096 kb on - strand, within speEat 136.148 kb on + strand, within speEat 136.189 kb on + strand, within speEat 136.191 kb on + strand, within speEat 136.257 kb on - strand, within speEat 136.402 kb on - strandat 136.402 kb on - strandat 136.424 kb on - strandat 136.516 kb on - strandat 136.516 kb on - strandat 136.733 kb on + strand, within yacCat 136.755 kb on - strand, within yacCat 136.779 kb on + strand, within yacCat 136.779 kb on + strand, within yacCat 136.859 kb on + strand, within yacCat 136.901 kb on - strandat 136.922 kb on + strandat 136.922 kb on + strandat 137.033 kb on + strandat 137.123 kb on + strandat 137.199 kb on + strandat 137.240 kb on + strand, within cueOat 137.240 kb on + strand, within cueOat 137.380 kb on - strand, within cueOat 137.401 kb on + strand, within cueOat 137.401 kb on - strand, within cueOat 137.401 kb on - strand, within cueOat 137.482 kb on - strand, within cueOat 137.485 kb on + strand, within cueOat 137.544 kb on - strand, within cueOat 137.544 kb on - strand, within cueOat 137.708 kb on + strand, within cueOat 137.708 kb on + strand, within cueOat 137.730 kb on - strand, within cueOat 137.730 kb on - strand, within cueOat 137.735 kb on - strand, within cueOat 137.852 kb on - strand, within cueOat 137.889 kb on + strand, within cueOat 137.897 kb on + strand, within cueOat 137.907 kb on + strand, within cueOat 137.913 kb on - strand, within cueOat 138.067 kb on + strand, within cueOat 138.067 kb on + strand, within cueOat 138.070 kb on + strand, within cueOat 138.070 kb on + strand, within cueOat 138.232 kb on + strand, within cueOat 138.242 kb on + strand, within cueOat 138.252 kb on + strand, within cueOat 138.255 kb on - strand, within cueOat 138.352 kb on - strand, within cueOat 138.418 kb on + strand, within cueOat 138.418 kb on + strand, within cueOat 138.435 kb on - strand, within cueOat 138.435 kb on - strand, within cueOat 138.566 kb on + strandat 138.566 kb on + strandat 138.572 kb on - strandat 138.589 kb on - strandat 138.689 kb on - strandat 138.797 kb on - strandat 138.862 kb on + strandat 138.862 kb on + strandat 138.870 kb on - strandat 138.870 kb on - strandat 138.870 kb on - strandat 138.870 kb on - strandat 138.919 kb on - strandat 138.928 kb on + strandat 138.928 kb on + strandat 138.950 kb on - strandat 139.025 kb on + strandat 139.025 kb on + strandat 139.057 kb on - strandat 139.095 kb on - strand, within gcdat 139.095 kb on - strand, within gcdat 139.147 kb on + strand, within gcdat 139.203 kb on + strand, within gcdat 139.211 kb on + strand, within gcdat 139.211 kb on + strand, within gcdat 139.243 kb on + strand, within gcdat 139.245 kb on - strand, within gcdat 139.245 kb on - strand, within gcdat 139.267 kb on + strand, within gcdat 139.274 kb on + strand, within gcdat 139.282 kb on - strand, within gcdat 139.282 kb on - strand, within gcdat 139.284 kb on + strand, within gcdat 139.284 kb on + strand, within gcdat 139.285 kb on - strand, within gcdat 139.285 kb on - strand, within gcdat 139.288 kb on + strand, within gcdat 139.352 kb on - strand, within gcdat 139.405 kb on - strand, within gcdat 139.405 kb on - strand, within gcdat 139.469 kb on - strand, within gcdat 139.469 kb on - strand, within gcdat 139.492 kb on - strand, within gcdat 139.492 kb on - strand, within gcdat 139.552 kb on + strand, within gcdat 139.623 kb on + strand, within gcdat 139.623 kb on + strand, within gcd

download strain data

Per-strain Table

Position Strand Gene LocusTag Fraction a-Ketoglutaric (C)
remove
136,088 + speE b0121 0.57 +0.6
136,096 - speE b0121 0.57 +0.3
136,148 + speE b0121 0.63 -1.1
136,189 + speE b0121 0.68 +0.4
136,191 + speE b0121 0.68 +0.6
136,257 - speE b0121 0.76 +0.1
136,402 - -1.3
136,402 - -1.2
136,424 - +0.1
136,516 - -1.6
136,516 - -0.1
136,733 + yacC b0122 0.47 +0.3
136,755 - yacC b0122 0.53 +0.1
136,779 + yacC b0122 0.60 +0.3
136,779 + yacC b0122 0.60 +0.8
136,859 + yacC b0122 0.83 -0.6
136,901 - +0.9
136,922 + -0.8
136,922 + +0.3
137,033 + -2.4
137,123 + +0.9
137,199 + +0.3
137,240 + cueO b0123 0.10 +0.4
137,240 + cueO b0123 0.10 +0.2
137,380 - cueO b0123 0.19 +1.1
137,401 + cueO b0123 0.21 -0.1
137,401 - cueO b0123 0.21 +0.7
137,401 - cueO b0123 0.21 -0.1
137,482 - cueO b0123 0.26 +1.0
137,485 + cueO b0123 0.26 +0.8
137,544 - cueO b0123 0.30 -2.4
137,544 - cueO b0123 0.30 -0.7
137,708 + cueO b0123 0.40 +0.3
137,708 + cueO b0123 0.40 +0.8
137,730 - cueO b0123 0.42 +1.2
137,730 - cueO b0123 0.42 +1.7
137,735 - cueO b0123 0.42 -0.6
137,852 - cueO b0123 0.50 +0.7
137,889 + cueO b0123 0.52 -0.0
137,897 + cueO b0123 0.52 -1.2
137,907 + cueO b0123 0.53 -0.5
137,913 - cueO b0123 0.54 +1.1
138,067 + cueO b0123 0.63 -0.9
138,067 + cueO b0123 0.63 -0.4
138,070 + cueO b0123 0.64 -0.3
138,070 + cueO b0123 0.64 +0.8
138,232 + cueO b0123 0.74 +4.0
138,242 + cueO b0123 0.75 +0.3
138,252 + cueO b0123 0.75 +0.2
138,255 - cueO b0123 0.76 +0.4
138,352 - cueO b0123 0.82 +0.1
138,418 + cueO b0123 0.86 -1.9
138,418 + cueO b0123 0.86 +0.1
138,435 - cueO b0123 0.87 +0.6
138,435 - cueO b0123 0.87 +0.4
138,566 + -0.3
138,566 + +0.0
138,572 - -0.1
138,589 - -0.0
138,689 - +0.2
138,797 - +0.8
138,862 + -1.6
138,862 + +0.1
138,870 - +0.7
138,870 - -0.0
138,870 - +0.3
138,870 - +0.0
138,919 - -1.2
138,928 + -0.5
138,928 + -1.2
138,950 - -0.1
139,025 + -1.0
139,025 + +2.0
139,057 - +0.1
139,095 - gcd b0124 0.11 +1.0
139,095 - gcd b0124 0.11 +0.7
139,147 + gcd b0124 0.13 -0.0
139,203 + gcd b0124 0.15 -1.1
139,211 + gcd b0124 0.16 -0.6
139,211 + gcd b0124 0.16 +0.3
139,243 + gcd b0124 0.17 +0.0
139,245 - gcd b0124 0.17 +0.3
139,245 - gcd b0124 0.17 +0.6
139,267 + gcd b0124 0.18 +0.9
139,274 + gcd b0124 0.18 +0.1
139,282 - gcd b0124 0.19 -0.0
139,282 - gcd b0124 0.19 +0.7
139,284 + gcd b0124 0.19 +0.3
139,284 + gcd b0124 0.19 +0.7
139,285 - gcd b0124 0.19 -1.0
139,285 - gcd b0124 0.19 +0.5
139,288 + gcd b0124 0.19 -0.2
139,352 - gcd b0124 0.22 -0.2
139,405 - gcd b0124 0.24 +0.7
139,405 - gcd b0124 0.24 +0.1
139,469 - gcd b0124 0.27 +0.7
139,469 - gcd b0124 0.27 +0.8
139,492 - gcd b0124 0.27 +0.5
139,492 - gcd b0124 0.27 +0.8
139,552 + gcd b0124 0.30 +0.0
139,623 + gcd b0124 0.33 -0.0
139,623 + gcd b0124 0.33 +0.1

Or see this region's nucleotide sequence