Experiment: a-Ketoglutaric (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt aceF and lpd are separated by 324 nucleotides lpd and yacH are separated by 70 nucleotides
b0115: aceF - dihydrolipoamide acetyltransferase (NCBI), at 125,695 to 127,587
aceF
b0116: lpd - dihydrolipoamide dehydrogenase (NCBI), at 127,912 to 129,336
lpd
b0117: yacH - hypothetical protein (NCBI), at 129,407 to 131,260
yacH
Position (kb) 127
128
129
130 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 127.735 kb on + strand at 127.735 kb on + strand at 127.768 kb on + strand at 127.893 kb on + strand at 127.898 kb on - strand at 129.309 kb on + strand at 129.309 kb on + strand at 129.429 kb on - strand at 129.521 kb on + strand at 129.521 kb on + strand at 129.617 kb on + strand, within yacH at 129.617 kb on + strand, within yacH at 129.644 kb on + strand, within yacH at 129.644 kb on + strand, within yacH at 129.647 kb on + strand, within yacH at 129.647 kb on + strand, within yacH at 129.649 kb on + strand, within yacH at 129.649 kb on + strand, within yacH at 129.657 kb on - strand, within yacH at 129.670 kb on - strand, within yacH at 129.675 kb on - strand, within yacH at 129.675 kb on - strand, within yacH at 129.692 kb on - strand, within yacH at 129.692 kb on - strand, within yacH at 129.776 kb on + strand, within yacH at 129.778 kb on + strand, within yacH at 129.839 kb on - strand, within yacH at 129.841 kb on + strand, within yacH at 129.841 kb on + strand, within yacH at 129.847 kb on - strand, within yacH at 129.867 kb on - strand, within yacH at 129.936 kb on + strand, within yacH at 130.001 kb on + strand, within yacH at 130.003 kb on + strand, within yacH at 130.041 kb on + strand, within yacH at 130.086 kb on + strand, within yacH at 130.109 kb on + strand, within yacH at 130.109 kb on + strand, within yacH at 130.136 kb on - strand, within yacH at 130.146 kb on - strand, within yacH at 130.151 kb on - strand, within yacH at 130.168 kb on - strand, within yacH at 130.173 kb on - strand, within yacH at 130.173 kb on - strand, within yacH at 130.180 kb on + strand, within yacH at 130.201 kb on - strand, within yacH at 130.272 kb on - strand, within yacH
Per-strain Table
Position Strand Gene LocusTag Fraction a-Ketoglutaric (C) remove 127,735 + +0.1 127,735 + -0.9 127,768 + -1.3 127,893 + +0.3 127,898 - -0.1 129,309 + +0.1 129,309 + -1.7 129,429 - +0.6 129,521 + -2.0 129,521 + -1.2 129,617 + yacH b0117 0.11 +0.3 129,617 + yacH b0117 0.11 +0.5 129,644 + yacH b0117 0.13 +0.2 129,644 + yacH b0117 0.13 +0.5 129,647 + yacH b0117 0.13 -1.2 129,647 + yacH b0117 0.13 -0.5 129,649 + yacH b0117 0.13 -0.6 129,649 + yacH b0117 0.13 -0.5 129,657 - yacH b0117 0.13 -0.4 129,670 - yacH b0117 0.14 -0.6 129,675 - yacH b0117 0.14 -2.1 129,675 - yacH b0117 0.14 -0.7 129,692 - yacH b0117 0.15 +0.3 129,692 - yacH b0117 0.15 +0.8 129,776 + yacH b0117 0.20 +0.9 129,778 + yacH b0117 0.20 +0.4 129,839 - yacH b0117 0.23 -0.1 129,841 + yacH b0117 0.23 +1.2 129,841 + yacH b0117 0.23 +0.3 129,847 - yacH b0117 0.24 +0.7 129,867 - yacH b0117 0.25 +0.5 129,936 + yacH b0117 0.29 +0.3 130,001 + yacH b0117 0.32 -0.5 130,003 + yacH b0117 0.32 -1.2 130,041 + yacH b0117 0.34 +1.2 130,086 + yacH b0117 0.37 +1.2 130,109 + yacH b0117 0.38 -1.8 130,109 + yacH b0117 0.38 -3.0 130,136 - yacH b0117 0.39 +0.7 130,146 - yacH b0117 0.40 +0.2 130,151 - yacH b0117 0.40 -0.7 130,168 - yacH b0117 0.41 -1.9 130,173 - yacH b0117 0.41 -0.1 130,173 - yacH b0117 0.41 +0.5 130,180 + yacH b0117 0.42 +1.6 130,201 - yacH b0117 0.43 +0.7 130,272 - yacH b0117 0.47 -0.2
Or see this region's nucleotide sequence