Experiment: a-Ketoglutaric (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yhbP and yhbQ are separated by 50 nucleotides yhbQ and yhbS overlap by 14 nucleotides yhbS and yhbT overlap by 7 nucleotides yhbT and yhbU are separated by 208 nucleotides
b3154: yhbP - hypothetical protein (NCBI), at 3,297,494 to 3,297,937
yhbP
b3155: yhbQ - hypothetical protein (NCBI), at 3,297,988 to 3,298,290
yhbQ
b3156: yhbS - predicted acyltransferase with acyl-CoA N-acyltransferase domain (NCBI), at 3,298,277 to 3,298,780
yhbS
b3157: yhbT - predicted lipid carrier protein (NCBI), at 3,298,774 to 3,299,298
yhbT
b3158: yhbU - predicted peptidase (collagenase-like) (NCBI), at 3,299,507 to 3,300,502
yhbU
Position (kb)
3298
3299
3300 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3297.842 kb on + strand, within yhbP at 3297.852 kb on + strand, within yhbP at 3297.857 kb on + strand, within yhbP at 3297.879 kb on + strand, within yhbP at 3297.889 kb on - strand, within yhbP at 3297.889 kb on - strand, within yhbP at 3297.960 kb on + strand at 3297.967 kb on + strand at 3297.967 kb on + strand at 3297.974 kb on - strand at 3298.077 kb on + strand, within yhbQ at 3298.077 kb on + strand, within yhbQ at 3298.121 kb on + strand, within yhbQ at 3298.129 kb on + strand, within yhbQ at 3298.129 kb on + strand, within yhbQ at 3298.238 kb on + strand, within yhbQ at 3298.238 kb on + strand, within yhbQ at 3298.249 kb on + strand, within yhbQ at 3298.298 kb on + strand at 3298.298 kb on + strand at 3298.304 kb on - strand at 3298.339 kb on + strand, within yhbS at 3298.411 kb on + strand, within yhbS at 3298.411 kb on + strand, within yhbS at 3298.427 kb on + strand, within yhbS at 3298.435 kb on - strand, within yhbS at 3298.459 kb on - strand, within yhbS at 3298.483 kb on - strand, within yhbS at 3298.485 kb on - strand, within yhbS at 3298.495 kb on - strand, within yhbS at 3298.497 kb on + strand, within yhbS at 3298.504 kb on - strand, within yhbS at 3298.578 kb on + strand, within yhbS at 3298.578 kb on + strand, within yhbS at 3298.591 kb on - strand, within yhbS at 3298.635 kb on - strand, within yhbS at 3298.635 kb on - strand, within yhbS at 3298.649 kb on - strand, within yhbS at 3298.724 kb on + strand, within yhbS at 3298.737 kb on + strand at 3298.737 kb on + strand at 3298.858 kb on + strand, within yhbT at 3298.864 kb on + strand, within yhbT at 3298.951 kb on - strand, within yhbT at 3299.033 kb on - strand, within yhbT at 3299.033 kb on - strand, within yhbT at 3299.115 kb on + strand, within yhbT at 3299.123 kb on - strand, within yhbT at 3299.123 kb on - strand, within yhbT at 3299.220 kb on - strand, within yhbT at 3299.399 kb on - strand at 3299.399 kb on - strand at 3299.515 kb on + strand at 3299.515 kb on + strand at 3299.550 kb on - strand at 3299.626 kb on + strand, within yhbU at 3299.785 kb on - strand, within yhbU at 3299.785 kb on - strand, within yhbU at 3299.809 kb on - strand, within yhbU at 3299.857 kb on + strand, within yhbU at 3299.860 kb on - strand, within yhbU at 3299.968 kb on + strand, within yhbU at 3299.968 kb on + strand, within yhbU at 3299.968 kb on + strand, within yhbU at 3299.981 kb on - strand, within yhbU at 3300.162 kb on + strand, within yhbU at 3300.216 kb on - strand, within yhbU at 3300.216 kb on - strand, within yhbU at 3300.225 kb on - strand, within yhbU at 3300.240 kb on - strand, within yhbU
Per-strain Table
Position Strand Gene LocusTag Fraction a-Ketoglutaric (C) remove 3,297,842 + yhbP b3154 0.78 -0.3 3,297,852 + yhbP b3154 0.81 -0.1 3,297,857 + yhbP b3154 0.82 +0.9 3,297,879 + yhbP b3154 0.87 +0.2 3,297,889 - yhbP b3154 0.89 +1.7 3,297,889 - yhbP b3154 0.89 +0.7 3,297,960 + +0.1 3,297,967 + -1.1 3,297,967 + +0.6 3,297,974 - +0.3 3,298,077 + yhbQ b3155 0.29 +0.6 3,298,077 + yhbQ b3155 0.29 +0.2 3,298,121 + yhbQ b3155 0.44 -0.1 3,298,129 + yhbQ b3155 0.47 -0.2 3,298,129 + yhbQ b3155 0.47 -0.3 3,298,238 + yhbQ b3155 0.83 -3.0 3,298,238 + yhbQ b3155 0.83 -2.5 3,298,249 + yhbQ b3155 0.86 +0.5 3,298,298 + -0.0 3,298,298 + +1.5 3,298,304 - -0.5 3,298,339 + yhbS b3156 0.12 +0.9 3,298,411 + yhbS b3156 0.27 +0.4 3,298,411 + yhbS b3156 0.27 -3.1 3,298,427 + yhbS b3156 0.30 +0.2 3,298,435 - yhbS b3156 0.31 +0.4 3,298,459 - yhbS b3156 0.36 -1.0 3,298,483 - yhbS b3156 0.41 +0.3 3,298,485 - yhbS b3156 0.41 +2.4 3,298,495 - yhbS b3156 0.43 +0.2 3,298,497 + yhbS b3156 0.44 +0.2 3,298,504 - yhbS b3156 0.45 +0.3 3,298,578 + yhbS b3156 0.60 +2.9 3,298,578 + yhbS b3156 0.60 +0.8 3,298,591 - yhbS b3156 0.62 -0.1 3,298,635 - yhbS b3156 0.71 +1.0 3,298,635 - yhbS b3156 0.71 +0.3 3,298,649 - yhbS b3156 0.74 +1.0 3,298,724 + yhbS b3156 0.89 -0.6 3,298,737 + +0.8 3,298,737 + -0.1 3,298,858 + yhbT b3157 0.16 +1.0 3,298,864 + yhbT b3157 0.17 -1.0 3,298,951 - yhbT b3157 0.34 +3.4 3,299,033 - yhbT b3157 0.49 +2.4 3,299,033 - yhbT b3157 0.49 +2.9 3,299,115 + yhbT b3157 0.65 -0.8 3,299,123 - yhbT b3157 0.66 +1.6 3,299,123 - yhbT b3157 0.66 +0.8 3,299,220 - yhbT b3157 0.85 +0.7 3,299,399 - +2.8 3,299,399 - +2.3 3,299,515 + -1.2 3,299,515 + -0.8 3,299,550 - +1.8 3,299,626 + yhbU b3158 0.12 -0.2 3,299,785 - yhbU b3158 0.28 +0.0 3,299,785 - yhbU b3158 0.28 +0.9 3,299,809 - yhbU b3158 0.30 -1.8 3,299,857 + yhbU b3158 0.35 -0.2 3,299,860 - yhbU b3158 0.35 +1.3 3,299,968 + yhbU b3158 0.46 +0.9 3,299,968 + yhbU b3158 0.46 +1.6 3,299,968 + yhbU b3158 0.46 +0.6 3,299,981 - yhbU b3158 0.48 -0.5 3,300,162 + yhbU b3158 0.66 -1.4 3,300,216 - yhbU b3158 0.71 +0.9 3,300,216 - yhbU b3158 0.71 +0.3 3,300,225 - yhbU b3158 0.72 -2.1 3,300,240 - yhbU b3158 0.74 -0.2
Or see this region's nucleotide sequence