Experiment: a-Ketoglutaric (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt uvrY and yecF are separated by 458 nucleotides yecF and sdiA are separated by 67 nucleotides sdiA and yecC are separated by 229 nucleotides yecC and yecS overlap by 4 nucleotides
b1914: uvrY - response regulator (NCBI), at 1,992,727 to 1,993,383
uvrY
b1915: yecF - hypothetical protein (NCBI), at 1,993,842 to 1,994,066
yecF
b1916: sdiA - DNA-binding transcriptional activator (NCBI), at 1,994,134 to 1,994,856
sdiA
b1917: yecC - predicted transporter subunit: ATP-binding component of ABC superfamily (NCBI), at 1,995,086 to 1,995,838
yecC
b1918: yecS - predicted transporter subunit: membrane component of ABC superfamily (NCBI), at 1,995,835 to 1,996,503
yecS
Position (kb)
1994
1995 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5
6 at 1993.136 kb on - strand, within uvrY at 1993.136 kb on - strand, within uvrY at 1993.220 kb on - strand, within uvrY at 1993.236 kb on + strand, within uvrY at 1993.236 kb on + strand, within uvrY at 1993.244 kb on - strand, within uvrY at 1993.244 kb on - strand, within uvrY at 1993.268 kb on - strand, within uvrY at 1993.309 kb on + strand, within uvrY at 1993.317 kb on + strand, within uvrY at 1993.328 kb on + strand at 1993.345 kb on + strand at 1993.350 kb on + strand at 1993.350 kb on + strand at 1993.358 kb on - strand at 1993.415 kb on - strand at 1993.419 kb on + strand at 1993.419 kb on - strand at 1993.522 kb on + strand at 1993.522 kb on + strand at 1993.522 kb on + strand at 1993.522 kb on + strand at 1993.534 kb on + strand at 1993.537 kb on + strand at 1993.540 kb on - strand at 1993.542 kb on - strand at 1993.548 kb on - strand at 1993.548 kb on - strand at 1993.549 kb on + strand at 1993.559 kb on + strand at 1993.559 kb on + strand at 1993.559 kb on + strand at 1993.559 kb on + strand at 1993.559 kb on + strand at 1993.559 kb on + strand at 1993.560 kb on + strand at 1993.560 kb on + strand at 1993.565 kb on + strand at 1993.571 kb on + strand at 1993.571 kb on + strand at 1993.571 kb on + strand at 1993.717 kb on + strand at 1993.717 kb on + strand at 1993.771 kb on + strand at 1993.771 kb on + strand at 1993.771 kb on + strand at 1993.772 kb on + strand at 1993.775 kb on + strand at 1993.775 kb on + strand at 1993.775 kb on + strand at 1993.777 kb on + strand at 1993.777 kb on + strand at 1993.777 kb on + strand at 1993.777 kb on + strand at 1993.786 kb on - strand at 1993.864 kb on - strand at 1993.864 kb on - strand at 1993.894 kb on - strand, within yecF at 1993.903 kb on + strand, within yecF at 1993.921 kb on + strand, within yecF at 1993.921 kb on + strand, within yecF at 1993.921 kb on + strand, within yecF at 1993.921 kb on + strand, within yecF at 1993.922 kb on - strand, within yecF at 1993.922 kb on - strand, within yecF at 1993.928 kb on + strand, within yecF at 1993.929 kb on - strand, within yecF at 1993.934 kb on + strand, within yecF at 1993.934 kb on + strand, within yecF at 1993.937 kb on + strand, within yecF at 1993.961 kb on + strand, within yecF at 1993.961 kb on + strand, within yecF at 1994.001 kb on + strand, within yecF at 1994.015 kb on - strand, within yecF at 1994.100 kb on + strand at 1994.110 kb on - strand at 1994.110 kb on - strand at 1994.120 kb on - strand at 1994.154 kb on - strand at 1994.154 kb on - strand at 1994.155 kb on - strand at 1994.249 kb on + strand, within sdiA at 1994.262 kb on + strand, within sdiA at 1994.268 kb on - strand, within sdiA at 1994.283 kb on - strand, within sdiA at 1994.332 kb on + strand, within sdiA at 1994.344 kb on - strand, within sdiA at 1994.344 kb on - strand, within sdiA at 1994.345 kb on - strand, within sdiA at 1994.357 kb on - strand, within sdiA at 1994.357 kb on - strand, within sdiA at 1994.364 kb on + strand, within sdiA at 1994.367 kb on - strand, within sdiA at 1994.367 kb on - strand, within sdiA at 1994.367 kb on - strand, within sdiA at 1994.378 kb on - strand, within sdiA at 1994.378 kb on - strand, within sdiA at 1994.381 kb on - strand, within sdiA at 1994.385 kb on + strand, within sdiA at 1994.434 kb on - strand, within sdiA at 1994.439 kb on + strand, within sdiA at 1994.492 kb on - strand, within sdiA at 1994.504 kb on - strand, within sdiA at 1994.509 kb on + strand, within sdiA at 1994.514 kb on + strand, within sdiA at 1994.517 kb on - strand, within sdiA at 1994.539 kb on - strand, within sdiA at 1994.539 kb on - strand, within sdiA at 1994.542 kb on + strand, within sdiA at 1994.549 kb on + strand, within sdiA at 1994.549 kb on + strand, within sdiA at 1994.562 kb on + strand, within sdiA at 1994.562 kb on + strand, within sdiA at 1994.582 kb on - strand, within sdiA at 1994.597 kb on - strand, within sdiA at 1994.605 kb on + strand, within sdiA at 1994.671 kb on - strand, within sdiA at 1994.674 kb on - strand, within sdiA at 1994.705 kb on - strand, within sdiA at 1994.705 kb on - strand, within sdiA at 1994.714 kb on - strand, within sdiA at 1994.749 kb on - strand, within sdiA at 1994.749 kb on - strand, within sdiA at 1994.801 kb on - strand at 1994.859 kb on - strand at 1994.956 kb on + strand at 1994.990 kb on - strand at 1994.990 kb on - strand at 1995.030 kb on + strand at 1995.312 kb on - strand, within yecC at 1995.312 kb on - strand, within yecC at 1995.592 kb on + strand, within yecC at 1995.598 kb on - strand, within yecC at 1995.598 kb on - strand, within yecC at 1995.688 kb on + strand, within yecC at 1995.771 kb on - strand at 1995.777 kb on - strand at 1995.777 kb on - strand at 1995.779 kb on + strand at 1995.779 kb on + strand at 1995.779 kb on + strand at 1995.779 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction a-Ketoglutaric (C) remove 1,993,136 - uvrY b1914 0.62 +1.2 1,993,136 - uvrY b1914 0.62 +1.1 1,993,220 - uvrY b1914 0.75 +0.1 1,993,236 + uvrY b1914 0.77 -0.6 1,993,236 + uvrY b1914 0.77 -0.5 1,993,244 - uvrY b1914 0.79 +1.6 1,993,244 - uvrY b1914 0.79 +1.5 1,993,268 - uvrY b1914 0.82 +0.2 1,993,309 + uvrY b1914 0.89 +0.2 1,993,317 + uvrY b1914 0.90 +2.0 1,993,328 + -0.8 1,993,345 + +0.3 1,993,350 + -1.7 1,993,350 + -1.5 1,993,358 - -0.2 1,993,415 - +0.9 1,993,419 + +1.2 1,993,419 - +0.1 1,993,522 + -0.3 1,993,522 + +0.0 1,993,522 + +0.8 1,993,522 + -1.4 1,993,534 + +0.2 1,993,537 + -0.3 1,993,540 - -0.3 1,993,542 - -1.7 1,993,548 - -1.7 1,993,548 - -0.9 1,993,549 + -1.1 1,993,559 + +1.0 1,993,559 + -0.3 1,993,559 + -0.1 1,993,559 + +0.1 1,993,559 + +0.1 1,993,559 + -0.1 1,993,560 + +0.8 1,993,560 + +0.0 1,993,565 + -0.4 1,993,571 + -1.8 1,993,571 + +0.1 1,993,571 + -0.7 1,993,717 + -0.5 1,993,717 + +0.5 1,993,771 + +0.6 1,993,771 + -1.2 1,993,771 + -0.5 1,993,772 + +0.1 1,993,775 + +0.2 1,993,775 + +1.5 1,993,775 + +0.4 1,993,777 + -0.4 1,993,777 + +0.7 1,993,777 + +0.5 1,993,777 + -0.8 1,993,786 - -0.1 1,993,864 - -1.7 1,993,864 - +1.2 1,993,894 - yecF b1915 0.23 +0.2 1,993,903 + yecF b1915 0.27 +0.8 1,993,921 + yecF b1915 0.35 -0.1 1,993,921 + yecF b1915 0.35 +0.5 1,993,921 + yecF b1915 0.35 +0.1 1,993,921 + yecF b1915 0.35 +0.4 1,993,922 - yecF b1915 0.36 -0.8 1,993,922 - yecF b1915 0.36 +0.3 1,993,928 + yecF b1915 0.38 +0.6 1,993,929 - yecF b1915 0.39 -0.2 1,993,934 + yecF b1915 0.41 -0.2 1,993,934 + yecF b1915 0.41 +0.8 1,993,937 + yecF b1915 0.42 -1.4 1,993,961 + yecF b1915 0.53 +0.6 1,993,961 + yecF b1915 0.53 +1.1 1,994,001 + yecF b1915 0.71 +1.3 1,994,015 - yecF b1915 0.77 +0.1 1,994,100 + +0.3 1,994,110 - +0.1 1,994,110 - +0.8 1,994,120 - +1.1 1,994,154 - +0.7 1,994,154 - +0.1 1,994,155 - +0.1 1,994,249 + sdiA b1916 0.16 -0.2 1,994,262 + sdiA b1916 0.18 +0.7 1,994,268 - sdiA b1916 0.19 +0.4 1,994,283 - sdiA b1916 0.21 -0.0 1,994,332 + sdiA b1916 0.27 -1.6 1,994,344 - sdiA b1916 0.29 +1.1 1,994,344 - sdiA b1916 0.29 +1.2 1,994,345 - sdiA b1916 0.29 +1.3 1,994,357 - sdiA b1916 0.31 +0.9 1,994,357 - sdiA b1916 0.31 +0.3 1,994,364 + sdiA b1916 0.32 +0.4 1,994,367 - sdiA b1916 0.32 +6.6 1,994,367 - sdiA b1916 0.32 +0.3 1,994,367 - sdiA b1916 0.32 +0.7 1,994,378 - sdiA b1916 0.34 +0.3 1,994,378 - sdiA b1916 0.34 +1.5 1,994,381 - sdiA b1916 0.34 +1.2 1,994,385 + sdiA b1916 0.35 +0.7 1,994,434 - sdiA b1916 0.41 +0.7 1,994,439 + sdiA b1916 0.42 +1.1 1,994,492 - sdiA b1916 0.50 +3.1 1,994,504 - sdiA b1916 0.51 +0.7 1,994,509 + sdiA b1916 0.52 -1.2 1,994,514 + sdiA b1916 0.53 +0.1 1,994,517 - sdiA b1916 0.53 +0.7 1,994,539 - sdiA b1916 0.56 +0.1 1,994,539 - sdiA b1916 0.56 -0.2 1,994,542 + sdiA b1916 0.56 +0.3 1,994,549 + sdiA b1916 0.57 +0.8 1,994,549 + sdiA b1916 0.57 +0.9 1,994,562 + sdiA b1916 0.59 +0.5 1,994,562 + sdiA b1916 0.59 +0.2 1,994,582 - sdiA b1916 0.62 +1.7 1,994,597 - sdiA b1916 0.64 +0.6 1,994,605 + sdiA b1916 0.65 +1.4 1,994,671 - sdiA b1916 0.74 +1.1 1,994,674 - sdiA b1916 0.75 -0.3 1,994,705 - sdiA b1916 0.79 +0.6 1,994,705 - sdiA b1916 0.79 -1.2 1,994,714 - sdiA b1916 0.80 -0.6 1,994,749 - sdiA b1916 0.85 -0.8 1,994,749 - sdiA b1916 0.85 +0.1 1,994,801 - +0.6 1,994,859 - +0.4 1,994,956 + +1.1 1,994,990 - +0.1 1,994,990 - -0.2 1,995,030 + +0.5 1,995,312 - yecC b1917 0.30 -1.6 1,995,312 - yecC b1917 0.30 -0.9 1,995,592 + yecC b1917 0.67 +0.2 1,995,598 - yecC b1917 0.68 -0.8 1,995,598 - yecC b1917 0.68 +0.1 1,995,688 + yecC b1917 0.80 -0.3 1,995,771 - -0.3 1,995,777 - -2.1 1,995,777 - -1.0 1,995,779 + +0.8 1,995,779 + -0.8 1,995,779 + -0.9 1,995,779 + -0.1
Or see this region's nucleotide sequence