Experiment: a-Ketoglutaric (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt amtB and tesB are separated by 48 nucleotides tesB and ybaY are separated by 217 nucleotides ybaY and ybaZ are separated by 30 nucleotides
b0451: amtB - ammonium transporter (NCBI), at 472,190 to 473,476
amtB
b0452: tesB - acyl-CoA thioesterase II (NCBI), at 473,525 to 474,385
tesB
b0453: ybaY - glycoprotein/polysaccharide metabolism (VIMSS), at 474,603 to 475,175
ybaY
b0454: ybaZ - predicted methyltransferase (NCBI), at 475,206 to 475,595
ybaZ
Position (kb) 473
474
475 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 472.546 kb on + strand, within amtB at 472.669 kb on + strand, within amtB at 472.727 kb on + strand, within amtB at 472.751 kb on + strand, within amtB at 472.828 kb on + strand, within amtB at 472.876 kb on + strand, within amtB at 472.876 kb on + strand, within amtB at 473.140 kb on + strand, within amtB at 473.280 kb on + strand, within amtB at 473.295 kb on - strand, within amtB at 473.420 kb on - strand at 473.420 kb on - strand at 473.549 kb on + strand at 473.549 kb on + strand at 473.549 kb on + strand at 473.575 kb on - strand at 473.575 kb on - strand at 473.586 kb on + strand at 473.644 kb on - strand, within tesB at 473.651 kb on - strand, within tesB at 473.651 kb on - strand, within tesB at 473.651 kb on - strand, within tesB at 473.651 kb on - strand, within tesB at 473.855 kb on + strand, within tesB at 473.855 kb on + strand, within tesB at 473.901 kb on - strand, within tesB at 474.002 kb on + strand, within tesB at 474.055 kb on - strand, within tesB at 474.065 kb on - strand, within tesB at 474.105 kb on + strand, within tesB at 474.135 kb on - strand, within tesB at 474.135 kb on - strand, within tesB at 474.143 kb on - strand, within tesB at 474.145 kb on - strand, within tesB at 474.169 kb on + strand, within tesB at 474.195 kb on + strand, within tesB at 474.303 kb on - strand at 474.390 kb on + strand at 474.403 kb on - strand at 474.464 kb on + strand at 474.464 kb on + strand at 474.521 kb on - strand at 474.609 kb on + strand at 474.648 kb on - strand at 474.730 kb on + strand, within ybaY at 474.752 kb on + strand, within ybaY at 474.752 kb on + strand, within ybaY at 474.796 kb on + strand, within ybaY at 474.796 kb on + strand, within ybaY at 474.835 kb on + strand, within ybaY at 474.857 kb on + strand, within ybaY at 474.912 kb on + strand, within ybaY at 474.956 kb on - strand, within ybaY at 474.956 kb on - strand, within ybaY at 474.966 kb on - strand, within ybaY at 474.989 kb on + strand, within ybaY at 475.003 kb on - strand, within ybaY at 475.003 kb on - strand, within ybaY at 475.074 kb on + strand, within ybaY at 475.133 kb on + strand at 475.153 kb on + strand at 475.188 kb on - strand at 475.189 kb on + strand at 475.210 kb on - strand at 475.293 kb on + strand, within ybaZ at 475.293 kb on - strand, within ybaZ at 475.365 kb on - strand, within ybaZ
Per-strain Table
Position Strand Gene LocusTag Fraction a-Ketoglutaric (C) remove 472,546 + amtB b0451 0.28 -0.1 472,669 + amtB b0451 0.37 -0.7 472,727 + amtB b0451 0.42 +0.2 472,751 + amtB b0451 0.44 -0.5 472,828 + amtB b0451 0.50 +0.5 472,876 + amtB b0451 0.53 -0.9 472,876 + amtB b0451 0.53 +0.7 473,140 + amtB b0451 0.74 +4.4 473,280 + amtB b0451 0.85 +0.3 473,295 - amtB b0451 0.86 +0.4 473,420 - -0.3 473,420 - +0.8 473,549 + +0.8 473,549 + -0.2 473,549 + -0.8 473,575 - +1.5 473,575 - +2.0 473,586 + -0.6 473,644 - tesB b0452 0.14 +0.2 473,651 - tesB b0452 0.15 +0.4 473,651 - tesB b0452 0.15 +0.5 473,651 - tesB b0452 0.15 -1.8 473,651 - tesB b0452 0.15 +0.6 473,855 + tesB b0452 0.38 +0.4 473,855 + tesB b0452 0.38 -0.1 473,901 - tesB b0452 0.44 -0.1 474,002 + tesB b0452 0.55 -1.0 474,055 - tesB b0452 0.62 -0.5 474,065 - tesB b0452 0.63 -0.2 474,105 + tesB b0452 0.67 -1.7 474,135 - tesB b0452 0.71 +0.5 474,135 - tesB b0452 0.71 +1.1 474,143 - tesB b0452 0.72 +0.6 474,145 - tesB b0452 0.72 -0.0 474,169 + tesB b0452 0.75 -0.5 474,195 + tesB b0452 0.78 +0.1 474,303 - +1.3 474,390 + -1.0 474,403 - -1.0 474,464 + -0.1 474,464 + +0.6 474,521 - -0.1 474,609 + +0.9 474,648 - -0.5 474,730 + ybaY b0453 0.22 -0.1 474,752 + ybaY b0453 0.26 +0.5 474,752 + ybaY b0453 0.26 +1.7 474,796 + ybaY b0453 0.34 +1.1 474,796 + ybaY b0453 0.34 -0.1 474,835 + ybaY b0453 0.40 +1.0 474,857 + ybaY b0453 0.44 -0.1 474,912 + ybaY b0453 0.54 +0.6 474,956 - ybaY b0453 0.62 +0.4 474,956 - ybaY b0453 0.62 -0.0 474,966 - ybaY b0453 0.63 +0.2 474,989 + ybaY b0453 0.67 +0.9 475,003 - ybaY b0453 0.70 +1.1 475,003 - ybaY b0453 0.70 +0.2 475,074 + ybaY b0453 0.82 +1.1 475,133 + +0.8 475,153 + +1.1 475,188 - -0.2 475,189 + -0.5 475,210 - +0.3 475,293 + ybaZ b0454 0.22 +0.5 475,293 - ybaZ b0454 0.22 +0.2 475,365 - ybaZ b0454 0.41 +0.3
Or see this region's nucleotide sequence