Experiment: KB with Carbenicillin 3.1 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3323 and Psyr_3324 are separated by 195 nucleotides Psyr_3324 and Psyr_3325 are separated by 11 nucleotides
Psyr_3323: Psyr_3323 - conserved hypothetical protein, at 3,974,587 to 3,975,678
_3323
Psyr_3324: Psyr_3324 - transcriptional regulator, TetR family, at 3,975,874 to 3,976,488
_3324
Psyr_3325: Psyr_3325 - fumarylacetoacetate hydrolase, at 3,976,500 to 3,977,795
_3325
Position (kb)
3975
3976
3977 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3974.913 kb on - strand, within Psyr_3323 at 3975.189 kb on + strand, within Psyr_3323 at 3975.269 kb on + strand, within Psyr_3323 at 3975.270 kb on - strand, within Psyr_3323 at 3975.653 kb on + strand at 3975.654 kb on - strand at 3975.729 kb on + strand at 3975.729 kb on + strand at 3975.729 kb on + strand at 3975.729 kb on + strand at 3975.763 kb on + strand at 3975.764 kb on - strand at 3975.764 kb on - strand at 3975.764 kb on - strand at 3975.767 kb on + strand at 3975.767 kb on + strand at 3975.767 kb on + strand at 3975.767 kb on + strand at 3975.768 kb on - strand at 3975.768 kb on - strand at 3975.872 kb on + strand at 3975.872 kb on + strand at 3975.873 kb on - strand at 3975.873 kb on - strand at 3975.873 kb on - strand at 3975.873 kb on - strand at 3975.873 kb on - strand at 3975.873 kb on - strand at 3976.015 kb on + strand, within Psyr_3324 at 3976.015 kb on + strand, within Psyr_3324 at 3976.015 kb on + strand, within Psyr_3324 at 3976.016 kb on - strand, within Psyr_3324 at 3976.016 kb on - strand, within Psyr_3324 at 3976.016 kb on - strand, within Psyr_3324 at 3976.016 kb on - strand, within Psyr_3324 at 3976.016 kb on - strand, within Psyr_3324 at 3976.471 kb on + strand at 3976.673 kb on - strand, within Psyr_3325 at 3976.701 kb on - strand, within Psyr_3325 at 3976.780 kb on + strand, within Psyr_3325 at 3976.780 kb on + strand, within Psyr_3325 at 3976.780 kb on + strand, within Psyr_3325 at 3976.781 kb on - strand, within Psyr_3325 at 3976.855 kb on - strand, within Psyr_3325 at 3976.913 kb on - strand, within Psyr_3325 at 3977.061 kb on + strand, within Psyr_3325 at 3977.061 kb on + strand, within Psyr_3325 at 3977.061 kb on + strand, within Psyr_3325 at 3977.061 kb on + strand, within Psyr_3325 at 3977.061 kb on + strand, within Psyr_3325 at 3977.061 kb on + strand, within Psyr_3325 at 3977.062 kb on - strand, within Psyr_3325 at 3977.062 kb on - strand, within Psyr_3325 at 3977.062 kb on - strand, within Psyr_3325 at 3977.062 kb on - strand, within Psyr_3325 at 3977.062 kb on - strand, within Psyr_3325 at 3977.062 kb on - strand, within Psyr_3325 at 3977.062 kb on - strand, within Psyr_3325 at 3977.105 kb on + strand, within Psyr_3325 at 3977.106 kb on - strand, within Psyr_3325 at 3977.106 kb on - strand, within Psyr_3325 at 3977.185 kb on + strand, within Psyr_3325 at 3977.186 kb on - strand, within Psyr_3325 at 3977.317 kb on + strand, within Psyr_3325 at 3977.317 kb on + strand, within Psyr_3325 at 3977.318 kb on - strand, within Psyr_3325 at 3977.318 kb on - strand, within Psyr_3325 at 3977.318 kb on - strand, within Psyr_3325 at 3977.318 kb on - strand, within Psyr_3325 at 3977.318 kb on - strand, within Psyr_3325 at 3977.318 kb on - strand, within Psyr_3325 at 3977.323 kb on + strand, within Psyr_3325 at 3977.324 kb on - strand, within Psyr_3325 at 3977.385 kb on - strand, within Psyr_3325 at 3977.393 kb on - strand, within Psyr_3325 at 3977.393 kb on - strand, within Psyr_3325
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Carbenicillin 3.1 ug/ml remove 3,974,913 - Psyr_3323 0.30 -0.0 3,975,189 + Psyr_3323 0.55 -0.8 3,975,269 + Psyr_3323 0.62 +2.9 3,975,270 - Psyr_3323 0.63 +1.6 3,975,653 + +0.8 3,975,654 - -0.6 3,975,729 + -0.2 3,975,729 + -2.0 3,975,729 + +0.6 3,975,729 + -0.7 3,975,763 + -2.2 3,975,764 - +0.1 3,975,764 - -0.0 3,975,764 - -1.0 3,975,767 + -0.2 3,975,767 + -1.2 3,975,767 + -1.2 3,975,767 + -1.0 3,975,768 - -1.2 3,975,768 - -1.0 3,975,872 + -0.5 3,975,872 + +0.1 3,975,873 - -1.5 3,975,873 - +0.3 3,975,873 - -0.1 3,975,873 - +0.6 3,975,873 - -1.5 3,975,873 - +1.3 3,976,015 + Psyr_3324 0.23 +0.2 3,976,015 + Psyr_3324 0.23 -1.0 3,976,015 + Psyr_3324 0.23 +0.4 3,976,016 - Psyr_3324 0.23 +2.3 3,976,016 - Psyr_3324 0.23 -1.5 3,976,016 - Psyr_3324 0.23 -0.7 3,976,016 - Psyr_3324 0.23 +0.1 3,976,016 - Psyr_3324 0.23 -0.2 3,976,471 + -2.6 3,976,673 - Psyr_3325 0.13 +2.9 3,976,701 - Psyr_3325 0.16 +1.7 3,976,780 + Psyr_3325 0.22 -1.5 3,976,780 + Psyr_3325 0.22 +0.5 3,976,780 + Psyr_3325 0.22 +0.8 3,976,781 - Psyr_3325 0.22 -2.5 3,976,855 - Psyr_3325 0.27 +1.1 3,976,913 - Psyr_3325 0.32 -0.4 3,977,061 + Psyr_3325 0.43 -0.9 3,977,061 + Psyr_3325 0.43 +1.3 3,977,061 + Psyr_3325 0.43 +0.6 3,977,061 + Psyr_3325 0.43 -1.0 3,977,061 + Psyr_3325 0.43 +0.6 3,977,061 + Psyr_3325 0.43 -0.4 3,977,062 - Psyr_3325 0.43 -0.9 3,977,062 - Psyr_3325 0.43 +1.2 3,977,062 - Psyr_3325 0.43 +0.8 3,977,062 - Psyr_3325 0.43 +1.3 3,977,062 - Psyr_3325 0.43 -0.8 3,977,062 - Psyr_3325 0.43 -0.2 3,977,062 - Psyr_3325 0.43 +0.5 3,977,105 + Psyr_3325 0.47 -1.0 3,977,106 - Psyr_3325 0.47 -0.7 3,977,106 - Psyr_3325 0.47 -0.5 3,977,185 + Psyr_3325 0.53 -0.4 3,977,186 - Psyr_3325 0.53 -0.1 3,977,317 + Psyr_3325 0.63 -0.7 3,977,317 + Psyr_3325 0.63 +1.3 3,977,318 - Psyr_3325 0.63 +0.8 3,977,318 - Psyr_3325 0.63 -1.8 3,977,318 - Psyr_3325 0.63 +0.3 3,977,318 - Psyr_3325 0.63 +1.1 3,977,318 - Psyr_3325 0.63 +0.2 3,977,318 - Psyr_3325 0.63 +0.1 3,977,323 + Psyr_3325 0.64 +1.2 3,977,324 - Psyr_3325 0.64 +0.3 3,977,385 - Psyr_3325 0.68 -0.7 3,977,393 - Psyr_3325 0.69 +1.3 3,977,393 - Psyr_3325 0.69 +0.5
Or see this region's nucleotide sequence