Experiment: KB with Carbenicillin 3.1 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2453 and Psyr_2454 overlap by 33 nucleotides Psyr_2454 and Psyr_2455 are separated by 29 nucleotides Psyr_2455 and Psyr_2456 are separated by 100 nucleotides Psyr_2456 and Psyr_2457 are separated by 27 nucleotides
Psyr_2453: Psyr_2453 - NUDIX hydrolase, at 2,841,626 to 2,842,462
_2453
Psyr_2454: Psyr_2454 - Protein of unknown function DUF81, at 2,842,430 to 2,843,272
_2454
Psyr_2455: Psyr_2455 - Oxidoreductase, N-terminal:Oxidoreductase, C-terminal, at 2,843,302 to 2,844,450
_2455
Psyr_2456: Psyr_2456 - Short-chain dehydrogenase/reductase SDR, at 2,844,551 to 2,845,318
_2456
Psyr_2457: Psyr_2457 - Aminoglycoside phosphotransferase, at 2,845,346 to 2,846,413
_2457
Position (kb)
2843
2844
2845 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2842.466 kb on + strand at 2842.548 kb on + strand, within Psyr_2454 at 2842.548 kb on + strand, within Psyr_2454 at 2842.548 kb on + strand, within Psyr_2454 at 2842.549 kb on - strand, within Psyr_2454 at 2842.593 kb on + strand, within Psyr_2454 at 2842.593 kb on + strand, within Psyr_2454 at 2842.593 kb on + strand, within Psyr_2454 at 2842.593 kb on + strand, within Psyr_2454 at 2842.593 kb on + strand, within Psyr_2454 at 2842.593 kb on + strand, within Psyr_2454 at 2842.594 kb on - strand, within Psyr_2454 at 2842.851 kb on + strand, within Psyr_2454 at 2842.851 kb on + strand, within Psyr_2454 at 2842.851 kb on + strand, within Psyr_2454 at 2842.851 kb on + strand, within Psyr_2454 at 2842.852 kb on - strand, within Psyr_2454 at 2842.852 kb on - strand, within Psyr_2454 at 2842.852 kb on - strand, within Psyr_2454 at 2843.002 kb on - strand, within Psyr_2454 at 2843.135 kb on + strand, within Psyr_2454 at 2843.275 kb on + strand at 2843.284 kb on + strand at 2843.284 kb on + strand at 2843.285 kb on - strand at 2843.418 kb on - strand, within Psyr_2455 at 2843.418 kb on - strand, within Psyr_2455 at 2843.440 kb on + strand, within Psyr_2455 at 2843.440 kb on + strand, within Psyr_2455 at 2843.441 kb on - strand, within Psyr_2455 at 2843.504 kb on + strand, within Psyr_2455 at 2843.652 kb on + strand, within Psyr_2455 at 2843.653 kb on - strand, within Psyr_2455 at 2843.788 kb on + strand, within Psyr_2455 at 2843.788 kb on + strand, within Psyr_2455 at 2843.788 kb on + strand, within Psyr_2455 at 2844.032 kb on + strand, within Psyr_2455 at 2844.032 kb on + strand, within Psyr_2455 at 2844.038 kb on + strand, within Psyr_2455 at 2844.039 kb on - strand, within Psyr_2455 at 2844.128 kb on + strand, within Psyr_2455 at 2844.128 kb on + strand, within Psyr_2455 at 2844.128 kb on + strand, within Psyr_2455 at 2844.128 kb on + strand, within Psyr_2455 at 2844.128 kb on + strand, within Psyr_2455 at 2844.128 kb on + strand, within Psyr_2455 at 2844.129 kb on - strand, within Psyr_2455 at 2844.208 kb on + strand, within Psyr_2455 at 2844.208 kb on + strand, within Psyr_2455 at 2844.595 kb on + strand at 2844.595 kb on + strand at 2844.596 kb on - strand at 2844.597 kb on + strand at 2844.597 kb on + strand at 2844.598 kb on - strand at 2844.598 kb on - strand at 2844.598 kb on - strand at 2844.598 kb on - strand at 2845.094 kb on + strand, within Psyr_2456 at 2845.095 kb on - strand, within Psyr_2456 at 2845.301 kb on + strand at 2845.301 kb on + strand at 2845.301 kb on + strand at 2845.301 kb on + strand at 2845.302 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Carbenicillin 3.1 ug/ml remove 2,842,466 + +0.3 2,842,548 + Psyr_2454 0.14 -0.9 2,842,548 + Psyr_2454 0.14 -2.9 2,842,548 + Psyr_2454 0.14 +3.6 2,842,549 - Psyr_2454 0.14 +0.1 2,842,593 + Psyr_2454 0.19 +1.1 2,842,593 + Psyr_2454 0.19 -0.4 2,842,593 + Psyr_2454 0.19 -1.2 2,842,593 + Psyr_2454 0.19 -2.2 2,842,593 + Psyr_2454 0.19 -1.6 2,842,593 + Psyr_2454 0.19 -1.8 2,842,594 - Psyr_2454 0.19 +0.6 2,842,851 + Psyr_2454 0.50 -0.4 2,842,851 + Psyr_2454 0.50 +2.0 2,842,851 + Psyr_2454 0.50 -2.1 2,842,851 + Psyr_2454 0.50 -0.8 2,842,852 - Psyr_2454 0.50 +0.5 2,842,852 - Psyr_2454 0.50 +2.0 2,842,852 - Psyr_2454 0.50 +1.3 2,843,002 - Psyr_2454 0.68 -1.0 2,843,135 + Psyr_2454 0.84 -0.5 2,843,275 + -0.1 2,843,284 + +0.6 2,843,284 + -1.4 2,843,285 - +0.6 2,843,418 - Psyr_2455 0.10 +0.6 2,843,418 - Psyr_2455 0.10 -1.2 2,843,440 + Psyr_2455 0.12 +1.6 2,843,440 + Psyr_2455 0.12 +0.4 2,843,441 - Psyr_2455 0.12 +0.3 2,843,504 + Psyr_2455 0.18 -0.7 2,843,652 + Psyr_2455 0.30 -1.4 2,843,653 - Psyr_2455 0.31 +0.6 2,843,788 + Psyr_2455 0.42 +0.7 2,843,788 + Psyr_2455 0.42 -0.7 2,843,788 + Psyr_2455 0.42 +1.6 2,844,032 + Psyr_2455 0.64 +1.9 2,844,032 + Psyr_2455 0.64 -0.6 2,844,038 + Psyr_2455 0.64 -0.1 2,844,039 - Psyr_2455 0.64 +1.3 2,844,128 + Psyr_2455 0.72 +1.4 2,844,128 + Psyr_2455 0.72 +0.8 2,844,128 + Psyr_2455 0.72 +0.1 2,844,128 + Psyr_2455 0.72 +2.1 2,844,128 + Psyr_2455 0.72 +0.5 2,844,128 + Psyr_2455 0.72 +0.3 2,844,129 - Psyr_2455 0.72 -1.9 2,844,208 + Psyr_2455 0.79 -0.6 2,844,208 + Psyr_2455 0.79 +0.9 2,844,595 + +0.6 2,844,595 + -0.6 2,844,596 - +0.8 2,844,597 + -1.4 2,844,597 + -1.0 2,844,598 - +0.6 2,844,598 - -1.0 2,844,598 - +0.2 2,844,598 - +1.9 2,845,094 + Psyr_2456 0.71 +0.4 2,845,095 - Psyr_2456 0.71 +0.2 2,845,301 + -0.2 2,845,301 + -0.9 2,845,301 + +0.8 2,845,301 + +2.6 2,845,302 - -0.0
Or see this region's nucleotide sequence