Experiment: KB with Carbenicillin 3.1 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1823 and Psyr_1824 are separated by 93 nucleotides Psyr_1824 and Psyr_1825 are separated by 100 nucleotides Psyr_1825 and Psyr_1826 are separated by 61 nucleotides Psyr_1826 and Psyr_1827 are separated by 137 nucleotides Psyr_1827 and Psyr_1828 are separated by 89 nucleotides
Psyr_1823: Psyr_1823 - Twin-arginine translocation pathway signal, at 2,103,171 to 2,104,856
_1823
Psyr_1824: Psyr_1824 - amino acid ABC transporter substrate-binding protein, PAAT family, at 2,104,950 to 2,105,744
_1824
Psyr_1825: Psyr_1825 - DNA polymerase III, tau subunit, at 2,105,845 to 2,108,055
_1825
Psyr_1826: Psyr_1826 - Conserved hypothetical protein 103, at 2,108,117 to 2,108,443
_1826
Psyr_1827: Psyr_1827 - SirA-like protein, at 2,108,581 to 2,108,835
_1827
Psyr_1828: Psyr_1828 - 4-phosphoerythronate dehydrogenase, at 2,108,925 to 2,110,067
_1828
Position (kb)
2105
2106
2107
2108
2109 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2104.907 kb on + strand at 2104.919 kb on - strand at 2104.919 kb on - strand at 2104.972 kb on + strand at 2104.973 kb on - strand at 2105.173 kb on + strand, within Psyr_1824 at 2105.173 kb on + strand, within Psyr_1824 at 2105.173 kb on + strand, within Psyr_1824 at 2105.174 kb on - strand, within Psyr_1824 at 2105.174 kb on - strand, within Psyr_1824 at 2105.174 kb on - strand, within Psyr_1824 at 2105.374 kb on + strand, within Psyr_1824 at 2105.375 kb on - strand, within Psyr_1824 at 2105.437 kb on + strand, within Psyr_1824 at 2105.437 kb on + strand, within Psyr_1824 at 2105.437 kb on + strand, within Psyr_1824 at 2105.438 kb on - strand, within Psyr_1824 at 2105.671 kb on - strand at 2105.671 kb on - strand at 2105.671 kb on - strand at 2105.671 kb on - strand at 2105.765 kb on - strand at 2108.111 kb on + strand at 2108.112 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Carbenicillin 3.1 ug/ml remove 2,104,907 + +0.2 2,104,919 - +0.8 2,104,919 - -1.4 2,104,972 + +1.8 2,104,973 - +2.4 2,105,173 + Psyr_1824 0.28 +1.4 2,105,173 + Psyr_1824 0.28 +0.7 2,105,173 + Psyr_1824 0.28 -2.4 2,105,174 - Psyr_1824 0.28 +1.4 2,105,174 - Psyr_1824 0.28 -0.6 2,105,174 - Psyr_1824 0.28 -2.3 2,105,374 + Psyr_1824 0.53 -2.4 2,105,375 - Psyr_1824 0.53 +0.1 2,105,437 + Psyr_1824 0.61 -2.0 2,105,437 + Psyr_1824 0.61 +1.4 2,105,437 + Psyr_1824 0.61 +0.5 2,105,438 - Psyr_1824 0.61 -3.1 2,105,671 - -0.5 2,105,671 - -0.8 2,105,671 - -1.0 2,105,671 - -1.6 2,105,765 - -1.4 2,108,111 + -0.5 2,108,112 - -0.9
Or see this region's nucleotide sequence