Experiment: KB with Carbenicillin 3.1 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1367 and Psyr_1368 are separated by 96 nucleotides Psyr_1368 and Psyr_1369 are separated by 53 nucleotides Psyr_1369 and Psyr_1370 overlap by 4 nucleotides
Psyr_1367: Psyr_1367 - Zinc-containing alcohol dehydrogenase superfamily, at 1,550,472 to 1,551,584
_1367
Psyr_1368: Psyr_1368 - Carboxylesterase, at 1,551,681 to 1,552,526
_1368
Psyr_1369: Psyr_1369 - 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase, at 1,552,580 to 1,553,053
_1369
Psyr_1370: Psyr_1370 - tRNA pseudouridine synthase D, TruD, at 1,553,050 to 1,554,108
_1370
Position (kb)
1552
1553
1554 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1551.634 kb on - strand at 1551.634 kb on - strand at 1551.643 kb on + strand at 1551.643 kb on + strand at 1551.643 kb on + strand at 1551.644 kb on - strand at 1551.644 kb on - strand at 1551.644 kb on - strand at 1551.644 kb on - strand at 1551.644 kb on - strand at 1551.655 kb on + strand at 1551.656 kb on - strand at 1551.657 kb on + strand at 1551.657 kb on + strand at 1551.657 kb on + strand at 1551.657 kb on + strand at 1551.657 kb on + strand at 1551.657 kb on + strand at 1551.657 kb on + strand at 1551.658 kb on - strand at 1551.658 kb on - strand at 1551.658 kb on - strand at 1551.658 kb on - strand at 1551.658 kb on - strand at 1551.696 kb on - strand at 1551.783 kb on + strand, within Psyr_1368 at 1551.783 kb on + strand, within Psyr_1368 at 1551.783 kb on + strand, within Psyr_1368 at 1551.783 kb on + strand, within Psyr_1368 at 1551.783 kb on + strand, within Psyr_1368 at 1551.784 kb on - strand, within Psyr_1368 at 1551.784 kb on - strand, within Psyr_1368 at 1551.828 kb on + strand, within Psyr_1368 at 1551.828 kb on + strand, within Psyr_1368 at 1551.828 kb on + strand, within Psyr_1368 at 1551.828 kb on + strand, within Psyr_1368 at 1551.829 kb on - strand, within Psyr_1368 at 1551.929 kb on + strand, within Psyr_1368 at 1552.003 kb on - strand, within Psyr_1368 at 1552.026 kb on + strand, within Psyr_1368 at 1552.027 kb on - strand, within Psyr_1368 at 1552.053 kb on + strand, within Psyr_1368 at 1552.053 kb on + strand, within Psyr_1368 at 1552.115 kb on + strand, within Psyr_1368 at 1552.255 kb on - strand, within Psyr_1368 at 1552.461 kb on + strand at 1552.461 kb on + strand at 1552.461 kb on + strand at 1552.462 kb on - strand at 1552.462 kb on - strand at 1552.462 kb on - strand at 1552.543 kb on + strand at 1553.084 kb on - strand at 1553.275 kb on + strand, within Psyr_1370 at 1553.530 kb on + strand, within Psyr_1370 at 1553.531 kb on - strand, within Psyr_1370 at 1553.587 kb on + strand, within Psyr_1370 at 1553.656 kb on + strand, within Psyr_1370 at 1553.657 kb on - strand, within Psyr_1370 at 1553.657 kb on - strand, within Psyr_1370 at 1553.878 kb on - strand, within Psyr_1370 at 1553.983 kb on + strand, within Psyr_1370
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Carbenicillin 3.1 ug/ml remove 1,551,634 - -0.1 1,551,634 - +0.7 1,551,643 + -2.3 1,551,643 + -2.5 1,551,643 + -0.1 1,551,644 - +0.7 1,551,644 - +0.1 1,551,644 - -1.1 1,551,644 - +0.4 1,551,644 - +1.2 1,551,655 + +0.6 1,551,656 - +0.4 1,551,657 + +0.9 1,551,657 + +0.2 1,551,657 + +0.1 1,551,657 + -0.1 1,551,657 + -0.1 1,551,657 + +1.1 1,551,657 + +1.3 1,551,658 - -0.1 1,551,658 - -1.1 1,551,658 - +0.6 1,551,658 - +0.1 1,551,658 - -0.6 1,551,696 - -0.1 1,551,783 + Psyr_1368 0.12 +0.4 1,551,783 + Psyr_1368 0.12 +1.4 1,551,783 + Psyr_1368 0.12 -1.6 1,551,783 + Psyr_1368 0.12 +0.4 1,551,783 + Psyr_1368 0.12 -0.9 1,551,784 - Psyr_1368 0.12 +0.3 1,551,784 - Psyr_1368 0.12 +1.2 1,551,828 + Psyr_1368 0.17 +0.7 1,551,828 + Psyr_1368 0.17 +1.2 1,551,828 + Psyr_1368 0.17 +1.6 1,551,828 + Psyr_1368 0.17 +0.1 1,551,829 - Psyr_1368 0.17 +0.2 1,551,929 + Psyr_1368 0.29 +0.7 1,552,003 - Psyr_1368 0.38 -1.3 1,552,026 + Psyr_1368 0.41 +1.4 1,552,027 - Psyr_1368 0.41 +0.3 1,552,053 + Psyr_1368 0.44 +2.1 1,552,053 + Psyr_1368 0.44 +0.7 1,552,115 + Psyr_1368 0.51 +0.9 1,552,255 - Psyr_1368 0.68 -0.7 1,552,461 + +1.8 1,552,461 + -0.9 1,552,461 + -0.1 1,552,462 - -1.1 1,552,462 - +0.8 1,552,462 - -1.6 1,552,543 + +1.1 1,553,084 - -1.1 1,553,275 + Psyr_1370 0.21 -0.2 1,553,530 + Psyr_1370 0.45 +0.5 1,553,531 - Psyr_1370 0.45 +0.6 1,553,587 + Psyr_1370 0.51 +1.1 1,553,656 + Psyr_1370 0.57 +0.8 1,553,657 - Psyr_1370 0.57 +0.1 1,553,657 - Psyr_1370 0.57 +0.6 1,553,878 - Psyr_1370 0.78 +0.5 1,553,983 + Psyr_1370 0.88 -1.5
Or see this region's nucleotide sequence