Experiment: KB with Carbenicillin 3.1 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1166 and Psyr_1167 are separated by 27 nucleotides Psyr_1167 and Psyr_1168 overlap by 8 nucleotides Psyr_1168 and Psyr_1169 are separated by 9 nucleotides
Psyr_1166: Psyr_1166 - Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase, at 1,319,643 to 1,320,659
_1166
Psyr_1167: Psyr_1167 - nucleoside ABC transporter membrane protein, at 1,320,687 to 1,321,607
_1167
Psyr_1168: Psyr_1168 - nucleoside ABC transporter membrane protein, at 1,321,600 to 1,322,709
_1168
Psyr_1169: Psyr_1169 - nucleoside-binding protein, at 1,322,719 to 1,323,828
_1169
Position (kb)
1321
1322
1323 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1320.751 kb on + strand at 1320.751 kb on + strand at 1320.751 kb on + strand at 1320.751 kb on + strand at 1320.752 kb on - strand at 1320.752 kb on - strand at 1320.752 kb on - strand at 1320.752 kb on - strand at 1320.775 kb on + strand at 1320.776 kb on - strand at 1320.776 kb on - strand at 1320.983 kb on - strand, within Psyr_1167 at 1320.983 kb on - strand, within Psyr_1167 at 1320.983 kb on - strand, within Psyr_1167 at 1321.671 kb on + strand at 1321.672 kb on - strand at 1322.072 kb on + strand, within Psyr_1168 at 1322.072 kb on + strand, within Psyr_1168 at 1322.072 kb on + strand, within Psyr_1168 at 1322.072 kb on + strand, within Psyr_1168 at 1322.072 kb on + strand, within Psyr_1168 at 1322.072 kb on + strand, within Psyr_1168 at 1322.072 kb on + strand, within Psyr_1168 at 1322.073 kb on - strand, within Psyr_1168 at 1322.073 kb on - strand, within Psyr_1168 at 1322.073 kb on - strand, within Psyr_1168 at 1322.073 kb on - strand, within Psyr_1168 at 1322.073 kb on - strand, within Psyr_1168 at 1322.073 kb on - strand, within Psyr_1168 at 1322.148 kb on + strand, within Psyr_1168 at 1322.148 kb on + strand, within Psyr_1168 at 1322.149 kb on - strand, within Psyr_1168 at 1322.149 kb on - strand, within Psyr_1168 at 1322.149 kb on - strand, within Psyr_1168 at 1322.149 kb on - strand, within Psyr_1168 at 1322.149 kb on - strand, within Psyr_1168 at 1322.149 kb on - strand, within Psyr_1168 at 1322.149 kb on - strand, within Psyr_1168 at 1322.149 kb on - strand, within Psyr_1168 at 1322.150 kb on + strand, within Psyr_1168 at 1322.150 kb on + strand, within Psyr_1168 at 1322.150 kb on + strand, within Psyr_1168 at 1322.150 kb on + strand, within Psyr_1168 at 1322.150 kb on + strand, within Psyr_1168 at 1322.150 kb on + strand, within Psyr_1168 at 1322.150 kb on + strand, within Psyr_1168 at 1322.150 kb on + strand, within Psyr_1168 at 1322.150 kb on + strand, within Psyr_1168 at 1322.150 kb on + strand, within Psyr_1168 at 1322.150 kb on + strand, within Psyr_1168 at 1322.150 kb on + strand, within Psyr_1168 at 1322.150 kb on + strand, within Psyr_1168 at 1322.150 kb on + strand, within Psyr_1168 at 1322.151 kb on - strand, within Psyr_1168 at 1322.151 kb on - strand, within Psyr_1168 at 1322.151 kb on - strand, within Psyr_1168 at 1322.151 kb on - strand, within Psyr_1168 at 1322.151 kb on - strand, within Psyr_1168 at 1322.151 kb on - strand, within Psyr_1168 at 1322.151 kb on - strand, within Psyr_1168 at 1322.151 kb on - strand, within Psyr_1168 at 1322.246 kb on + strand, within Psyr_1168 at 1322.247 kb on - strand, within Psyr_1168 at 1322.247 kb on - strand, within Psyr_1168 at 1322.695 kb on + strand at 1322.696 kb on - strand at 1323.054 kb on - strand, within Psyr_1169 at 1323.163 kb on + strand, within Psyr_1169 at 1323.164 kb on - strand, within Psyr_1169 at 1323.164 kb on - strand, within Psyr_1169 at 1323.387 kb on - strand, within Psyr_1169 at 1323.684 kb on - strand, within Psyr_1169
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Carbenicillin 3.1 ug/ml remove 1,320,751 + -0.6 1,320,751 + +1.2 1,320,751 + -1.2 1,320,751 + -1.2 1,320,752 - +0.4 1,320,752 - +1.0 1,320,752 - -1.4 1,320,752 - -1.4 1,320,775 + +0.1 1,320,776 - -1.9 1,320,776 - +0.4 1,320,983 - Psyr_1167 0.32 -2.5 1,320,983 - Psyr_1167 0.32 +2.7 1,320,983 - Psyr_1167 0.32 -1.8 1,321,671 + -0.2 1,321,672 - +0.5 1,322,072 + Psyr_1168 0.43 -0.8 1,322,072 + Psyr_1168 0.43 -0.7 1,322,072 + Psyr_1168 0.43 -0.5 1,322,072 + Psyr_1168 0.43 -1.6 1,322,072 + Psyr_1168 0.43 -0.1 1,322,072 + Psyr_1168 0.43 +0.6 1,322,072 + Psyr_1168 0.43 -0.9 1,322,073 - Psyr_1168 0.43 -0.5 1,322,073 - Psyr_1168 0.43 -0.9 1,322,073 - Psyr_1168 0.43 -0.9 1,322,073 - Psyr_1168 0.43 +1.4 1,322,073 - Psyr_1168 0.43 -1.2 1,322,073 - Psyr_1168 0.43 +0.9 1,322,148 + Psyr_1168 0.49 -1.8 1,322,148 + Psyr_1168 0.49 -0.7 1,322,149 - Psyr_1168 0.49 +1.7 1,322,149 - Psyr_1168 0.49 -0.9 1,322,149 - Psyr_1168 0.49 +0.2 1,322,149 - Psyr_1168 0.49 +0.3 1,322,149 - Psyr_1168 0.49 +1.9 1,322,149 - Psyr_1168 0.49 -1.0 1,322,149 - Psyr_1168 0.49 -1.3 1,322,149 - Psyr_1168 0.49 -0.3 1,322,150 + Psyr_1168 0.50 -2.5 1,322,150 + Psyr_1168 0.50 +0.8 1,322,150 + Psyr_1168 0.50 +0.2 1,322,150 + Psyr_1168 0.50 +0.2 1,322,150 + Psyr_1168 0.50 +0.5 1,322,150 + Psyr_1168 0.50 +0.2 1,322,150 + Psyr_1168 0.50 -0.5 1,322,150 + Psyr_1168 0.50 -1.9 1,322,150 + Psyr_1168 0.50 -0.4 1,322,150 + Psyr_1168 0.50 +1.2 1,322,150 + Psyr_1168 0.50 -0.3 1,322,150 + Psyr_1168 0.50 +0.8 1,322,150 + Psyr_1168 0.50 -0.3 1,322,150 + Psyr_1168 0.50 -0.2 1,322,151 - Psyr_1168 0.50 -2.4 1,322,151 - Psyr_1168 0.50 -1.9 1,322,151 - Psyr_1168 0.50 +0.5 1,322,151 - Psyr_1168 0.50 +0.7 1,322,151 - Psyr_1168 0.50 +0.1 1,322,151 - Psyr_1168 0.50 +3.0 1,322,151 - Psyr_1168 0.50 -1.4 1,322,151 - Psyr_1168 0.50 +1.4 1,322,246 + Psyr_1168 0.58 +0.7 1,322,247 - Psyr_1168 0.58 +0.9 1,322,247 - Psyr_1168 0.58 -1.6 1,322,695 + -0.2 1,322,696 - +0.7 1,323,054 - Psyr_1169 0.30 -1.2 1,323,163 + Psyr_1169 0.40 -1.2 1,323,164 - Psyr_1169 0.40 +0.5 1,323,164 - Psyr_1169 0.40 -0.0 1,323,387 - Psyr_1169 0.60 +0.8 1,323,684 - Psyr_1169 0.87 -0.0
Or see this region's nucleotide sequence