Experiment: KB with Carbenicillin 3.1 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0821 and Psyr_0822 overlap by 1 nucleotides Psyr_0822 and Psyr_0823 are separated by 12 nucleotides Psyr_0823 and Psyr_0824 are separated by 39 nucleotides
Psyr_0821: Psyr_0821 - PTS system D-fructose-specific IIA component (F1P-forming), Frc family / Phosphocarrier protein HPr / phosphoenolpyruvate--protein phosphotransferase, at 926,782 to 929,652
_0821
Psyr_0822: Psyr_0822 - 1-phosphofructokinase, at 929,652 to 930,593
_0822
Psyr_0823: Psyr_0823 - PTS system D-fructose-specific IIB component (F1P-forming), Frc family / PTS system D-fructose-specific IIC component (F1P-forming), Frc family, at 930,606 to 932,348
_0823
Psyr_0824: Psyr_0824 - acetyl-CoA acetyltransferase, at 932,388 to 933,566
_0824
Position (kb) 930
931
932
933 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 929.650 kb on + strand at 929.782 kb on + strand, within Psyr_0822 at 929.782 kb on + strand, within Psyr_0822 at 929.782 kb on + strand, within Psyr_0822 at 929.782 kb on + strand, within Psyr_0822 at 929.782 kb on + strand, within Psyr_0822 at 929.782 kb on + strand, within Psyr_0822 at 929.783 kb on - strand, within Psyr_0822 at 929.928 kb on - strand, within Psyr_0822 at 930.571 kb on + strand at 930.572 kb on - strand at 930.591 kb on + strand at 930.591 kb on + strand at 930.592 kb on - strand at 930.592 kb on - strand at 930.619 kb on + strand at 930.619 kb on + strand at 930.619 kb on + strand at 930.620 kb on - strand at 930.620 kb on - strand at 930.620 kb on - strand at 930.623 kb on + strand at 930.623 kb on + strand at 930.624 kb on - strand at 930.656 kb on - strand at 931.003 kb on + strand, within Psyr_0823 at 931.197 kb on + strand, within Psyr_0823 at 931.197 kb on + strand, within Psyr_0823 at 931.197 kb on + strand, within Psyr_0823 at 931.198 kb on - strand, within Psyr_0823 at 931.743 kb on + strand, within Psyr_0823 at 931.743 kb on + strand, within Psyr_0823 at 931.744 kb on - strand, within Psyr_0823 at 931.744 kb on - strand, within Psyr_0823 at 931.744 kb on - strand, within Psyr_0823 at 931.744 kb on - strand, within Psyr_0823 at 931.744 kb on - strand, within Psyr_0823 at 931.744 kb on - strand, within Psyr_0823 at 931.744 kb on - strand, within Psyr_0823 at 931.744 kb on - strand, within Psyr_0823 at 931.744 kb on - strand, within Psyr_0823 at 931.744 kb on - strand, within Psyr_0823 at 931.744 kb on - strand, within Psyr_0823 at 931.744 kb on - strand, within Psyr_0823 at 931.744 kb on - strand, within Psyr_0823 at 931.927 kb on - strand, within Psyr_0823 at 931.927 kb on - strand, within Psyr_0823 at 931.958 kb on + strand, within Psyr_0823 at 932.163 kb on + strand, within Psyr_0823 at 932.163 kb on + strand, within Psyr_0823 at 932.164 kb on - strand, within Psyr_0823 at 932.245 kb on - strand at 932.289 kb on + strand at 932.289 kb on + strand at 932.290 kb on - strand at 932.290 kb on - strand at 932.389 kb on + strand at 932.778 kb on - strand, within Psyr_0824 at 932.784 kb on - strand, within Psyr_0824 at 933.065 kb on - strand, within Psyr_0824 at 933.104 kb on - strand, within Psyr_0824 at 933.104 kb on - strand, within Psyr_0824 at 933.199 kb on + strand, within Psyr_0824 at 933.199 kb on + strand, within Psyr_0824 at 933.199 kb on + strand, within Psyr_0824 at 933.200 kb on - strand, within Psyr_0824 at 933.200 kb on - strand, within Psyr_0824 at 933.200 kb on - strand, within Psyr_0824
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Carbenicillin 3.1 ug/ml remove 929,650 + -1.7 929,782 + Psyr_0822 0.14 -0.5 929,782 + Psyr_0822 0.14 +1.5 929,782 + Psyr_0822 0.14 +0.1 929,782 + Psyr_0822 0.14 +0.4 929,782 + Psyr_0822 0.14 +0.4 929,782 + Psyr_0822 0.14 -1.0 929,783 - Psyr_0822 0.14 -0.6 929,928 - Psyr_0822 0.29 -0.7 930,571 + +1.4 930,572 - +0.5 930,591 + -1.7 930,591 + -0.1 930,592 - -0.7 930,592 - -0.4 930,619 + +0.7 930,619 + -0.4 930,619 + -1.2 930,620 - -0.2 930,620 - +0.3 930,620 - +0.3 930,623 + +0.4 930,623 + +4.0 930,624 - +0.7 930,656 - +0.5 931,003 + Psyr_0823 0.23 -2.4 931,197 + Psyr_0823 0.34 +1.4 931,197 + Psyr_0823 0.34 +0.9 931,197 + Psyr_0823 0.34 +3.3 931,198 - Psyr_0823 0.34 -0.5 931,743 + Psyr_0823 0.65 -2.3 931,743 + Psyr_0823 0.65 +2.2 931,744 - Psyr_0823 0.65 -0.2 931,744 - Psyr_0823 0.65 +0.8 931,744 - Psyr_0823 0.65 -0.9 931,744 - Psyr_0823 0.65 -0.5 931,744 - Psyr_0823 0.65 +0.6 931,744 - Psyr_0823 0.65 +0.3 931,744 - Psyr_0823 0.65 -1.2 931,744 - Psyr_0823 0.65 +0.4 931,744 - Psyr_0823 0.65 -0.4 931,744 - Psyr_0823 0.65 -0.0 931,744 - Psyr_0823 0.65 -2.0 931,744 - Psyr_0823 0.65 +0.3 931,744 - Psyr_0823 0.65 -1.9 931,927 - Psyr_0823 0.76 -2.2 931,927 - Psyr_0823 0.76 -0.7 931,958 + Psyr_0823 0.78 +1.8 932,163 + Psyr_0823 0.89 +0.7 932,163 + Psyr_0823 0.89 +0.1 932,164 - Psyr_0823 0.89 +0.1 932,245 - +0.6 932,289 + -0.8 932,289 + +1.6 932,290 - -0.9 932,290 - -0.1 932,389 + -0.7 932,778 - Psyr_0824 0.33 +0.8 932,784 - Psyr_0824 0.34 -0.3 933,065 - Psyr_0824 0.57 +0.9 933,104 - Psyr_0824 0.61 +1.3 933,104 - Psyr_0824 0.61 -1.9 933,199 + Psyr_0824 0.69 +1.7 933,199 + Psyr_0824 0.69 +0.5 933,199 + Psyr_0824 0.69 +1.3 933,200 - Psyr_0824 0.69 -1.7 933,200 - Psyr_0824 0.69 -1.9 933,200 - Psyr_0824 0.69 +2.2
Or see this region's nucleotide sequence