Experiment: KB with Carbenicillin 3.1 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0146 and Psyr_0147 are separated by 284 nucleotides Psyr_0147 and Psyr_0148 are separated by 49 nucleotides Psyr_0148 and Psyr_0149 are separated by 77 nucleotides
Psyr_0146: Psyr_0146 - 4-aminobutyrate aminotransferase apoenzyme, at 158,304 to 159,608
_0146
Psyr_0147: Psyr_0147 - D-isomer specific 2-hydroxyacid dehydrogenase, NAD-binding protein, at 159,893 to 160,822
_0147
Psyr_0148: Psyr_0148 - hypothetical protein, at 160,872 to 161,111
_0148
Psyr_0149: Psyr_0149 - succinate semialdehyde dehydrogenase, at 161,189 to 162,646
_0149
Position (kb) 159
160
161 Strain fitness (log2 ratio) -2
-1
0
1
2 at 159.068 kb on - strand, within Psyr_0146 at 159.128 kb on - strand, within Psyr_0146 at 159.128 kb on - strand, within Psyr_0146 at 159.167 kb on + strand, within Psyr_0146 at 159.613 kb on - strand at 159.613 kb on - strand at 159.613 kb on - strand at 159.614 kb on + strand at 159.614 kb on + strand at 159.614 kb on + strand at 159.614 kb on + strand at 159.614 kb on + strand at 159.614 kb on + strand at 159.614 kb on + strand at 159.614 kb on + strand at 159.614 kb on + strand at 159.614 kb on + strand at 159.615 kb on - strand at 159.615 kb on - strand at 159.615 kb on - strand at 159.615 kb on - strand at 159.615 kb on - strand at 159.615 kb on - strand at 159.686 kb on + strand at 159.686 kb on + strand at 159.687 kb on - strand at 159.908 kb on + strand at 159.909 kb on - strand at 159.909 kb on - strand at 159.909 kb on - strand at 160.020 kb on - strand, within Psyr_0147 at 160.124 kb on - strand, within Psyr_0147 at 160.187 kb on + strand, within Psyr_0147 at 160.333 kb on + strand, within Psyr_0147 at 160.333 kb on + strand, within Psyr_0147 at 160.333 kb on + strand, within Psyr_0147 at 160.334 kb on - strand, within Psyr_0147 at 160.415 kb on + strand, within Psyr_0147 at 160.415 kb on + strand, within Psyr_0147 at 160.820 kb on + strand at 160.820 kb on + strand at 160.820 kb on + strand at 160.820 kb on + strand at 160.821 kb on - strand at 160.857 kb on + strand at 160.857 kb on + strand at 160.858 kb on - strand at 161.014 kb on - strand, within Psyr_0148 at 161.014 kb on - strand, within Psyr_0148 at 161.014 kb on - strand, within Psyr_0148 at 161.014 kb on - strand, within Psyr_0148 at 161.068 kb on - strand, within Psyr_0148 at 161.096 kb on + strand at 161.190 kb on + strand at 161.190 kb on + strand at 161.190 kb on + strand at 161.191 kb on - strand at 161.191 kb on - strand at 161.226 kb on + strand at 161.227 kb on - strand at 161.227 kb on - strand at 161.227 kb on - strand at 161.241 kb on + strand at 161.388 kb on + strand, within Psyr_0149 at 161.388 kb on + strand, within Psyr_0149 at 161.388 kb on + strand, within Psyr_0149 at 161.389 kb on - strand, within Psyr_0149 at 161.389 kb on - strand, within Psyr_0149 at 161.389 kb on - strand, within Psyr_0149 at 161.525 kb on + strand, within Psyr_0149 at 161.525 kb on + strand, within Psyr_0149 at 161.526 kb on - strand, within Psyr_0149 at 161.734 kb on - strand, within Psyr_0149 at 161.734 kb on - strand, within Psyr_0149
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Carbenicillin 3.1 ug/ml remove 159,068 - Psyr_0146 0.59 -0.7 159,128 - Psyr_0146 0.63 -0.3 159,128 - Psyr_0146 0.63 +0.7 159,167 + Psyr_0146 0.66 -0.5 159,613 - -0.4 159,613 - +0.1 159,613 - +0.1 159,614 + -1.2 159,614 + +0.2 159,614 + -0.4 159,614 + -0.4 159,614 + -0.5 159,614 + -0.5 159,614 + -2.0 159,614 + +1.3 159,614 + +0.7 159,614 + -0.0 159,615 - +1.7 159,615 - -1.2 159,615 - -1.7 159,615 - -0.1 159,615 - -1.9 159,615 - -0.2 159,686 + -0.6 159,686 + +2.4 159,687 - -0.5 159,908 + -1.2 159,909 - +1.2 159,909 - -1.3 159,909 - -1.4 160,020 - Psyr_0147 0.14 -2.2 160,124 - Psyr_0147 0.25 +0.4 160,187 + Psyr_0147 0.32 +1.0 160,333 + Psyr_0147 0.47 -0.1 160,333 + Psyr_0147 0.47 -0.4 160,333 + Psyr_0147 0.47 +0.7 160,334 - Psyr_0147 0.47 +1.4 160,415 + Psyr_0147 0.56 -0.4 160,415 + Psyr_0147 0.56 +1.1 160,820 + +0.5 160,820 + +0.9 160,820 + -0.3 160,820 + +0.0 160,821 - -1.9 160,857 + +0.4 160,857 + +0.1 160,858 - -0.6 161,014 - Psyr_0148 0.59 -0.3 161,014 - Psyr_0148 0.59 +0.4 161,014 - Psyr_0148 0.59 -1.4 161,014 - Psyr_0148 0.59 -0.4 161,068 - Psyr_0148 0.82 -1.3 161,096 + +0.6 161,190 + +1.2 161,190 + -1.2 161,190 + -1.7 161,191 - +0.1 161,191 - +0.3 161,226 + -0.4 161,227 - -2.6 161,227 - +0.7 161,227 - +0.5 161,241 + +1.9 161,388 + Psyr_0149 0.14 -2.7 161,388 + Psyr_0149 0.14 -1.4 161,388 + Psyr_0149 0.14 -2.0 161,389 - Psyr_0149 0.14 -0.4 161,389 - Psyr_0149 0.14 -0.6 161,389 - Psyr_0149 0.14 +1.1 161,525 + Psyr_0149 0.23 +1.2 161,525 + Psyr_0149 0.23 +0.4 161,526 - Psyr_0149 0.23 -0.2 161,734 - Psyr_0149 0.37 +0.3 161,734 - Psyr_0149 0.37 -1.3
Or see this region's nucleotide sequence